Analysis Tools
muscle
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• 1 of 42 tumors is a rhabdomyosarcoma
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mortality/aging
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• by day 7 after myocardial infarction, homozygotes have significantly better survival rate than wild-type
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• 50% of mice are dead by 21-22 weeks
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• associated with tumor formation
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neoplasm
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• develop fatal spontaneous tumors by 10 months of age
(J:108183)
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• about 80% of mice succumb to malignant lymphomas, chiefly thymic in origin
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• 3 of 42 tumors are carcinomas
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• 9% of all tumors seen in mice are sarcomas
• 1 of 42 tumors is an undifferentiated sarcoma
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• 1 of 42 tumors is a rhabdomyosarcoma
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• 2 of 42 tumors are osteosarcomas
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skeleton
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• 2 of 42 tumors are osteosarcomas
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cellular
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• after 14 weeks of selection with L-aspartic acid, N-(phosphonoacetyl) (PALA, an assay for gene amplification) homozygous MEFs develop resistance to up to 500uM PALA unlike wild-type cells which do not develop resistance
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• retroviral transfection of homozygous MEFs with an activated ras allele produces obvious transformed cells that are able to form colonies in soft agar, unlike wild-type MEFs where the introduction of 2 activated oncogenes is needed for transformation
(J:57882)
• the number of colonies produced in the soft agar assay is greater than in transfected homozygous Gadd45a null MEFs
(J:57882)
• confluent 2nd passage renal medullary cells show reduced viability 16 hours after osmolality is increased above 640 mosmol/kg water by addition of urea of NaCl
(J:110650)
• survival is less than wild-type when NaCl is added at 1040 or 1240 mosmol/kg water
(J:110650)
• survival is less than wild-type when urea is added at 840 mosmol/kg water or above
(J:110650)
• enhanced glycolysis in mouse embryonic fibroblasts
(J:186159)
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• MEFs fail to arrest after exposure to 6 Gy or 12 Gy irradiation
(J:118244)
• more mouse embryonic fibroblasts are in the S-phase compared with wild-type cells
(J:175018)
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• mouse embryonic cells (MEFs) fail to arrest at G1 upon exposure to gamma radiation and the G1/S-phase checkpoint is defective in gamma-irradiated thymic cells
(J:78491)
• mouse embryonic fibroblast cells fail to arrest in G1 following exposure to ionizing radiation as do wild-type cells
(J:126186)
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• following ionizing radiation no apoptosis is seen in homozygous MEFs unlike wild-type cells
(J:57882)
• adriamycin-induced apoptosis is significantly reduced or absent at both low and high concentrations of adriamycin compared to wild-type cells
(J:118244)
• mouse embryonic fibroblast cells fail to undergo apoptosis following exposure to ionizing radiation as do wild-type cells
(J:126186)
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• after exposure to 10-Gy ionizing radiation, 78.5% of CD4/CD8 double positive T cells survive compared to 45.5% in wild-type
(J:118602)
• irradiated mouse embryonic fibroblasts exhibit fail to exhibit a reduction in S-phase populations compared with similarly treated wild-type cells
(J:175018)
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• unlike wild-type epidermal cells that show a reduction in proliferation 24 hours post ionizing radiation, mutant epidermal cells exhibit no reduction in proliferation
(J:78491)
• mouse embryonic fibroblast cells fail to arrest in G1 or undergo apoptosis following exposure to ionizing radiation as do wild-type cells
(J:126186)
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• MEFs transfected with retrovirus encoding a puromycin resistance gene are resistant to adriamycin-induced apoptosis compared to transfected wild-type cells
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• gamma-irradiation-induced apoptosis is virtually abrogated
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• proliferation in the outer medullas of triple knockouts
(J:110650)
• primary fibroblasts proliferated faster than wild-type or Ing1-null cells in culture
(J:118602)
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• low passage MEFs have a plating efficiency of about 1.6% compared to 0.005% in wild-type cells, 0.025% in Gadd45a null cells, 1.2% in Cdkn1a null cells, and up to 1.4% in Gadd45a Cdkn1a double null cells
(J:57882)
• in mouse embryonic fibroblasts
(J:175018)
• in mouse embryonic fibroblasts
(J:186159)
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• in mouse embryonic fibroblasts
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• Ras-transfected mouse embryonic fibroblasts fail to exhibit senescence unlike similarly treated wild-type cells
(J:175018)
• in mouse embryonic fibroblasts
(J:186159)
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• increased reactive oxygen species in mouse embryonic fibroblasts
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cardiovascular system
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• homozygotes exhibit a better survival rate than wild-type and have a lower incidence of left ventricular rupture despite comparable infarct size, heart rate, and mean arterial blood pressure
• exhibit a thicker infarct wall and show fewer numbers of apoptotic cells in the infarct area than in wild-type after myocardial infarction
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homeostasis/metabolism
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• homozygotes exhibit a better survival rate than wild-type and have a lower incidence of left ventricular rupture despite comparable infarct size, heart rate, and mean arterial blood pressure
• exhibit a thicker infarct wall and show fewer numbers of apoptotic cells in the infarct area than in wild-type after myocardial infarction
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hematopoietic system
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• CD4+/CD8+ thymocytes are resistant to Trp53-mediated death unlike wild-type cells
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• gamma-irradiation-induced apoptosis is virtually abrogated
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immune system
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• CD4+/CD8+ thymocytes are resistant to Trp53-mediated death unlike wild-type cells
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• gamma-irradiation-induced apoptosis is virtually abrogated
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endocrine/exocrine glands
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• CD4+/CD8+ thymocytes are resistant to Trp53-mediated death unlike wild-type cells
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• gamma-irradiation-induced apoptosis is virtually abrogated
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