Kmt2a^tm1.1Mlc
Targeted Allele Detail

Summary

• Symbol: Kmt2a^tm1.1Mlc
• Name: lysine (K)-specific methyltransferase 2A; targeted mutation 1.1, Michael L Cleary
• MGI ID: MGI:5317820
• Synonyms: MLL^dC
• Gene: Kmt2a Location: Chr9:44714652-44792594 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Kmt2a^tm1.1Mlc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183048
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 28 was replaced with a modified exon 28 in which nucleotide substitution (GAT to TAA) resulted in the amino acid substitution of a stop codon for aspartic acid at the second processing site. This mutation prevents the expression of the C-terminus subunit. Western blot analysis confirmed the lack of expression of the C-terminal subunit and reduced expression of the N-terminus subunit. Cre-mediated recombination removed the neo cassette. (J:183048)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2a Mutation: 137 strains or lines available

References

• Original: J:183048 Yokoyama A, et al., Proteolytically cleaved MLL subunits are susceptible to distinct degradation pathways. J Cell Sci. 2011 Jul 1;124(Pt 13):2208-19
• All: 2 reference(s)