Pole4^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Pole4^{tm1(KOMP)Vlcg}
• Name: polymerase (DNA-directed), epsilon 4 (p12 subunit); targeted mutation 1, Velocigene
• MGI ID: MGI:5085645
• Synonyms: Pole4^-
• Gene: Pole4 Location: Chr6:82593513-82629985 bp, - strand Genetic Position: Chr6, 35.94 cM, cytoband D1
• Alliance: Pole4^{tm1(KOMP)Vlcg} page
• IMPC: Pole4 gene page

Mutation origin

• Mutant Cell Lines: 18012A-C12, 18012A-F2, 18012A-F3, 18012A-G4, 18012A-G8
• Germline Transmission: Earliest citation of germline transmission: J:261973
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 4875bp between positions 82624943-82629817 of Chromosome 6 (Build GRCm39). Sequencing of the first exon revealed that the deletion cassette is inserted 94 bp after the start of exon 1. Western blot analysis confirmed loss of protein expression in E13.5 homozygous mutant embryos. (J:136110, J:261973)

Expression

In Structures Affected by this Mutation:

19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Pole4 Mutation: 7 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 5 reference(s)