|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• median survival of 10 weeks
|
|
• large tumors with metastasis to lymph nodes, livers and/or kidney
|
|
• large tumors with metastasis to lymph nodes, livers and/or kidney
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• in DN4 and CD4SP cells following the TCR beta selection checkpoint
|
|
• 75% reduction, cell-autonomous
|
|
• thymocyte chemotaxis is impaired
|
|
• slight
|
|
• reduction is specific to TCR alpha-beta cells
• in the periphery
• however, the number of gamma-delta cells is normal
|
|
• in the thymus
|
|
• in the thymus
|
|
• in DN4 and CD4SP cells following the TCR beta selection checkpoint
|
|
• 75% reduction, cell-autonomous
|
|
• thymocyte chemotaxis is impaired
|
|
• slight
|
|
• reduction is specific to TCR alpha-beta cells
• in the periphery
• however, the number of gamma-delta cells is normal
|
|
• in the thymus
|
|
• in the thymus
|
|
• 75% reduction, cell-autonomous
|
|
• in DN4 and CD4SP cells following the TCR beta selection checkpoint
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells
|
|
• in the thymus
|
|
• modestly in the periphery
• in the thymus
|
|
• modestly in the periphery
• in the thymus
|
|
• of mature single positive cells in the thymus
|
|
• polyclonal T cells exhibit attenuated response to an antigen compared with wild-type cells
|
|
• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells
|
|
• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells
|
|
• in the thymus
|
|
• modestly in the periphery
• in the thymus
|
|
• modestly in the periphery
• in the thymus
|
|
• of mature single positive cells in the thymus
|
|
• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells
|
|
• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells
|
|
• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells
|
|
• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• in the thymus
|
|
• in the thymus
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• in the thymus
|
|
• in the thymus
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal thymic cellularity and development
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• CD4+ T cells have less impairment of in vitro proliferation when a RhoA activator is used
|
|
• CD4+ T cells have less impairment of in vitro proliferation when a RhoA activator is used
|
|
• CD4+ T cells have less impairment of in vitro proliferation when a RhoA activator is used
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased 5 fold
|
|
• reduced about 2 fold in peripheral blood
|
|
• total number of thymocytes significantly reduced
|
|
• reduced in numbers but normal numbers of CD8+ cells
|
|
• appear as early as 16 weeks of age
|
|
• increased 5 fold
|
|
• reduced about 2 fold in peripheral blood
|
|
• total number of thymocytes significantly reduced
|
|
• reduced in numbers but normal numbers of CD8+ cells
|
|
• total number of thymocytes significantly reduced
|
|
• appear as early as 16 weeks of age
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency
|
|
• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency
|
|
• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• proliferating T cells fail to exhibit the same increase in spontaneous apoptosis observed in Brca2tm1Mak/Brca2tm2Mak Tg(Lck-cre)548Jxm mice
|
|
• likely due to decreased apoptosis
|
|
• tumorigenesis is accelerated compared to in wild-type and Trp53tm1Brd/Trp53tm1Brd Tg(Lck-cre)548Jxm mice but not compared to in Trp53tm1Brd homozygotes
|
|
• 90% of mice succumb to T-cell lymphomas compared to 70% of Trp53tm1Brd/Trp53tm1Brd Tg(Lck-cre)548Jxm mice
|
|
• mice develop high grade malignancies
|
|
• T cells exhibit increased frequencies of chromatid or chromosomal breaks, tri-radial structures, and chromosomal fragments compared to in Brca2tm1Mak/Brca2tm2Mak Tg(Lck-cre)548Jxm mice or wild-type cells
|
|
• thymocytes are resistant to ionizing radiation-induced cell death unlike wild-type cells
• however, response to UV- or methyl methane sulfonate-induced cell death is normal
|
|
• likely due to decreased apoptosis
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• die at an average age of 51.7 weeks, however lethality is sometimes seen as early as 14 weeks of age
|
|
• peripheral T cell lymphopenia is seen early in life but is no longer apparent in older mutants
|
|
• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased B-to-T cell ratio
|
|
• lymphoproliferation in older mutants is confirmed by increased total lymphocyte counts
|
|
• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery
|
|
• the CD4+ to CD8+ T-cell ratio is increased in spleen, lymph node, and peripheral blood
(J:82759)
• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased proportion of CD8+ T cells relative to CD4+ T cells
(J:107455)
|
|
• relative proportion of splenic and lymph node T cells is significantly lower in young mutants
|
|
• lower numbers of CD4+ T cells in young mutants
|
|
• lower numbers of CD8+ T cells in young mutants
|
|
• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery
|
|
• older mutants accumulate nonclonal T cell infiltrates in the lungs, liver, and kidneys accompanied by tissue damage
• T cell infiltration is seen as early as 20 weeks of age and progresses as mice age
|
|
• thymocytes and activated T cells are resistant to CD95L-induced apoptosis, however mitochondria-mediated apoptosis is normal
|
|
• do not exhibit an expansion of CD8+ T cells in the peripheral blood 6 days after lymphocytic choriomeningitis virus (LCMV) infection and show decreased representation of CD62LhighCD44low (memory) T cells
• LCMV-infected mutants are unable to generate CD8+ cytotoxic T cells specific for LCMV
|
|
• isolated T cells from old mutants are in a perpetual state of activation
|
|
• T cells derived from older mutants exhibit defective in vitro proliferative responses to various stimuli and activated T cells are resistant to CD95L-induced apoptosis
|
|
• exhibit defective activation-induced expansion of peripheral T cells
|
|
• in older mutants
|
|
• lymphoid hyperplasia is apparent in older mutants
|
|
• peripheral T cell lymphopenia is seen early in life but is no longer apparent in older mutants
|
|
• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased B-to-T cell ratio
|
|
• lymphoproliferation in older mutants is confirmed by increased total lymphocyte counts
|
|
• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery
|
|
• the CD4+ to CD8+ T-cell ratio is increased in spleen, lymph node, and peripheral blood
(J:82759)
• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased proportion of CD8+ T cells relative to CD4+ T cells
(J:107455)
|
|
• relative proportion of splenic and lymph node T cells is significantly lower in young mutants
|
|
• lower numbers of CD4+ T cells in young mutants
|
|
• lower numbers of CD8+ T cells in young mutants
|
|
• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery
|
|
• older mutants accumulate nonclonal T cell infiltrates in the lungs, liver, and kidneys accompanied by tissue damage
• T cell infiltration is seen as early as 20 weeks of age and progresses as mice age
|
|
• thymocytes and activated T cells are resistant to CD95L-induced apoptosis, however mitochondria-mediated apoptosis is normal
|
|
• do not exhibit an expansion of CD8+ T cells in the peripheral blood 6 days after lymphocytic choriomeningitis virus (LCMV) infection and show decreased representation of CD62LhighCD44low (memory) T cells
• LCMV-infected mutants are unable to generate CD8+ cytotoxic T cells specific for LCMV
|
|
• isolated T cells from old mutants are in a perpetual state of activation
|
|
• T cells derived from older mutants exhibit defective in vitro proliferative responses to various stimuli and activated T cells are resistant to CD95L-induced apoptosis
|
|
• exhibit defective activation-induced expansion of peripheral T cells
|
|
• livers from 30-week old mutants display T cell accumulation as focal perivascular infiltrates
|
|
• kidneys from 30-week old mutants show focal interstitial and periglomerular T cell infiltration
|
|
• lungs from 30-week old mutants contain obvious interstitial peribronchiovascular T cell infiltration
• at about 1 year of age, disrupted lung tissue organization is associated with much more widespread and abundant T cell infiltration
|
|
• thymocytes and activated T cells are resistant to CD95L-induced apoptosis, however mitochondria-mediated apoptosis is normal
|
|
• T cells derived from older mutants exhibit defective in vitro proliferative responses to various stimuli and activated T cells are resistant to CD95L-induced apoptosis
|
|
• exhibit defective activation-induced expansion of peripheral T cells
|
Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| autoimmune lymphoproliferative syndrome type 2B | DOID:0110116 |
OMIM:607271 |
J:107455 | |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• total number of thymocytes significantly reduced
|
|
• increased 3 fold
|
|
• half the normal numbers of single positive thymocyte
• proportion of CD4+ to CD8+ is normal in spleen and peripheral blood
|
|
• total number of thymocytes significantly reduced
|
|
• increased 3 fold
|
|
• half the normal numbers of single positive thymocyte
• proportion of CD4+ to CD8+ is normal in spleen and peripheral blood
|
|
• total number of thymocytes significantly reduced
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• drop in number of single positive thymocytes comparable to the increase in double negative cells
• no increase in CD8+ single positive thymocytes
• near absence of single positive thymocytes in the periphery
|
|
• 10 fold increase in the percentage of double negative thymocytes
|
|
• near absence in the periphery
|
|
• near absence of single positive thymocytes in the periphery
• drop in number of single positive thymocytes comparable to the increase in double negative cells
• no increase in CD8+ single positive thymocytes
|
|
• 10 fold increase in the percentage of double negative thymocytes
|
|
• near absence in the periphery
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• thymic lymphomas develop with a mean age of onset of 60 days, similar to that seen in T cell-specific Pten homozygotes
|
|
• thymic lymphomas develop with a mean age of onset of 60 days, similar to that seen in T cell-specific Pten homozygotes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• significant reduction in size of the thymus with cellularity decreased almost 3-fold
• the percentage of apoptotic cells in the thymus is doubled
|
|
• unproductive transcripts with premature stop codons is detectable in 29% of single positive T cells
|
|
• there is a 3-fold reduction in the number of double positive T cells found in the thymus
|
|
• there is a 4- to 5-fold reduction in CD4 T cells found in the thymus
• the number of CD4 T cells found in the periphery is 30% that of wild-type
|
|
• there is a 2-fold to 3-fold reduction in T cell numbers
• the number of CD8 T cells found in the periphery is 30% that of wild-type
|
|
• significant reduction in size of the thymus with cellularity decreased almost 3-fold
• the percentage of apoptotic cells in the thymus is doubled
|
|
• unproductive transcripts with premature stop codons is detectable in 29% of single positive T cells
|
|
• there is a 3-fold reduction in the number of double positive T cells found in the thymus
|
|
• there is a 4- to 5-fold reduction in CD4 T cells found in the thymus
• the number of CD4 T cells found in the periphery is 30% that of wild-type
|
|
• there is a 2-fold to 3-fold reduction in T cell numbers
• the number of CD8 T cells found in the periphery is 30% that of wild-type
|
|
• significant reduction in size of the thymus with cellularity decreased almost 3-fold
• the percentage of apoptotic cells in the thymus is doubled
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• T cell development and proliferation are normal
|
|
• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells
• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal
|
| N |
• response to DNA damaging agents is normal
|
|
• T cells exhibit increased frequencies of chromatid or chromosomal breaks, tri-radial structures, and chromosomal fragments compared to in wild-type cells
|
|
• 14.5% of T cells exhibit aneuploidy unlike wild-type cells
|
|
• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells
• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal
|
|
• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells
• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• reduced proliferation when stimulated with monoclonal antibody to CD3e
|
|
• increased proportion of double negative thymocytes
• absolute number of double negative thymocytes is normal
|
|
• less than 10% of number in controls
|
|
• less than 10% of number in controls
|
|
• 90% reduction in thymocyte number
|
|
• fewer T cells entering S phase
|
|
• reduced viability of thymocytes in culture
• reduced survival of T cells when stimulated with monoclonal antibody to CD3e
|
|
• increased thymocyte sensitivity of gamma-irradiation
|
|
• reduced proliferation when stimulated with monoclonal antibody to CD3e
|
|
• only about 12% with tumors
• most tumors are peripheral rather than thymic
|
|
• reduced proliferation when stimulated with monoclonal antibody to CD3e
|
|
• 90% reduction in thymocyte number
|
|
• increased proportion of double negative thymocytes
• absolute number of double negative thymocytes is normal
|
|
• less than 10% of number in controls
|
|
• less than 10% of number in controls
|
|
• 90% reduction in thymocyte number
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased incidence
• reduced latency
|
|
• most mice with thymic lymphomas are moribund or die by 3 months of age
|
|
• increased frequency of aberrations such as polyploidy, quadriradial chromosomes, and telomeric translocations
|
| N |
• thymus cellularity restored
• T cell numbers in lymph nodes are improved
|
|
• increased incidence
• reduced latency
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased frequency of aberrations such as polyploidy, quadriradial chromosomes, and telomeric translocations
|
| N |
• thymus cellularity restored
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• T cell numbers in lymph nodes are more nearly normal
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• 32% with lymphomas by 180 days of age
• tumors are restricted to the thymus
|
| N |
• complete thymocyte restoration
• T cell numbers in the periphery are restored
|
| N |
• resistant to gamma irradiation
• restoration of normal cell cycle arrest of T cells when stimulated with antibody to CD3e or CD3 and Il2
|
|
• increased frequency
|
|
• 32% with lymphomas by 180 days of age
• tumors are restricted to the thymus
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• thymus cellularity is reduced about 75%
|
|
• the absolute number of double positive cells is decreased about 80%
|
|
• the absolute number of CD4 single positive cells is decreased about 80%
|
|
• thymus cellularity is reduced about 75%
|
|
• the absolute number of double positive cells is decreased about 80%
|
|
• the absolute number of CD4 single positive cells is decreased about 80%
|
|
• thymus cellularity is reduced about 75%
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• thymus cellularity is reduced
|
|
• the absolute number of double positive cells is decreased
|
|
• the absolute number of CD4 single positive cells is decreased
|
|
• thymus cellularity is reduced
|
|
• the absolute number of double positive cells is decreased
|
|
• the absolute number of CD4 single positive cells is decreased
|
|
• thymus cellularity is reduced
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• T cells either do not leave thymus or fail to migrate into the blood and to the peripheral lymphoid organs
|
|
• T cells either do not leave thymus or fail to migrate into the blood and to the peripheral lymphoid organs
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• cellularity of the thymus is reduced to 11% of controls
|
|
• thymocytes accumulate at the DN3 stage
• reduced numbers of cells at the DN4 stage
|
|
• double positive cell population reduced to 62% of normal
|
|
• cellularity of the thymus is reduced to 11% of controls
|
|
• thymocytes accumulate at the DN3 stage
• reduced numbers of cells at the DN4 stage
|
|
• double positive cell population reduced to 62% of normal
|
|
• cellularity of the thymus is reduced to 11% of controls
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• increase in annexin-V expression in single positive T cells
|
|
|
• about a 75% reduction in the number of T cells in the lymph nodes and spleen
• no T cells carrying the recombined allele are found in the lymph nodes or spleen
|
|
|
• about a 50% reduction in thymocyte cell number
|
|
|
• cortical double positive T cells are reduced by 50%
|
|
|
• reduced by 50%
|
|
|
• reduced by 75%
|
|
|
• increase in annexin-V expression in single positive T cells
|
|
|
• about a 75% reduction in the number of T cells in the lymph nodes and spleen
• no T cells carrying the recombined allele are found in the lymph nodes or spleen
|
|
|
• about a 50% reduction in thymocyte cell number
|
|
|
• cortical double positive T cells are reduced by 50%
|
|
|
• reduced by 50%
|
|
|
• reduced by 75%
|
|
|
• increase in annexin-V expression in single positive T cells
|
|
|
• about a 50% reduction in thymocyte cell number
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• at 15 months as in Bcl2l11tm1.1Ast homozygotes
|
|
• at 15 months as in Bcl2l11tm1.1Ast homozygotes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• in DN and DP thymocytes
|
|
• a severely hypoplastic medulla
|
|
• an 80-90% reduction
|
|
• a marked decrease in the proportion of DP and SP thymocytes
• DN thymocytes percentage and absolute numbers were not significantly altered
|
|
• a marked decrease in the proportion of DP and SP thymocytes
|
|
• in DN and DP thymocytes
|
|
• a severely hypoplastic medulla
|
|
• an 80-90% reduction
|
|
• a marked decrease in the proportion of DP and SP thymocytes
• DN thymocytes percentage and absolute numbers were not significantly altered
|
|
• a marked decrease in the proportion of DP and SP thymocytes
|
|
• cell cycle arrest at S/G2/M phase in DN thymocyte
|
|
• in DN and DP thymocytes
|
|
• a severely hypoplastic medulla
|
|
• an 80-90% reduction
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• an 80-90% reduction compared to control
|
|
• an 80-90% reduction compared to control
|
|
• an 80-90% reduction compared to control
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• homeostatic CD4+ T cell proliferation is increased within the lymph nodes
• allogenic stimulation of CD4+ T cells is also increased
|
|
• thymus weight and cellularity is increased by about a third
|
|
• CD4+ T cell numbers in the lymph nodes and blood are increased by about a third
|
|
• CD8+ T cell numbers in the lymph nodes are slightly increased
|
|
• CD4+ T cells have longer interactions between themselves and with allogenic dendritic cells
• CD4+ T cells have a higher affinity for ICAM-coated surfaces
• CD4+ T cells have decreased motility through transwell filters
• CD4+ T cells transferred into wild-type mice have enhanced enhanced transendothelial migration into lymph nodes
|
|
• axillary, cervical, inguinal and mesenteric lymph nodes are increased in weight and cell number by about a third
|
|
• an increase in ear thickness is observed after sensitization and challenge with DNFB
• CD4+ T cells in cervical lymph nodes have a higher proliferation rate than those of control mice after DNFB challenge
|
|
• IL-2 secretion is enhanced by CD4+ T cells in response to allogenic stimulation
|
|
• diabetes development is accelerated and aggravated after streptozotocin induction with high glucose levels detected
• peripancreatic lymph nodes are enlarged 5-6 days after disease induction
• increased numbers of CD4+ T cells are noted in the pancreas 14 days after disease induction compared to controls
|
|
• homeostatic CD4+ T cell proliferation is increased within the lymph nodes
• allogenic stimulation of CD4+ T cells is also increased
|
|
• thymus weight and cellularity is increased by about a third
|
|
• CD4+ T cell numbers in the lymph nodes and blood are increased by about a third
|
|
• CD8+ T cell numbers in the lymph nodes are slightly increased
|
|
• CD4+ T cells have longer interactions between themselves and with allogenic dendritic cells
• CD4+ T cells have a higher affinity for ICAM-coated surfaces
• CD4+ T cells have decreased motility through transwell filters
• CD4+ T cells transferred into wild-type mice have enhanced enhanced transendothelial migration into lymph nodes
|
|
• thymus weight and cellularity is increased by about a third
|
|
• homeostatic CD4+ T cell proliferation is increased within the lymph nodes
• allogenic stimulation of CD4+ T cells is also increased
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Lung and salivary gland inflammation in Tgfb1tm2.1Doe/Tgfb1tm2.1Doe Tg(Lck-cre)548Jxm/? (b,e) and Tgfb1tm1Doe/Tgfb1tm2.1Doe Tg(Lck-cre)548Jxm/? (c,f) mice
|
• salivary gland inflammation is observed in mice at 5 months of age
|
|
• inflammation is observed in the lungs at 5 months of age
|
|
• inflammation is observed in the lungs at 5 months of age
|
|
• salivary gland inflammation is observed in mice at 5 months of age
|
|
• salivary gland inflammation is observed in mice at 5 months of age
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice
|
|
• thymus contain 5% of the cells found in wild-type mice
|
|
• T cell development is blocked between DN2/3 and DN4 stages
• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice
|
|
• 5% to 10% of wild-type
|
|
• 40% of wild-type
|
|
• T cell numbers in the spleen are 40% to 50% of wild-type
|
|
• 5% to 10% of wild-type
|
|
• 5% to 10% of wild-type
|
|
• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice
|
|
• thymus contain 5% of the cells found in wild-type mice
|
|
• T cell development is blocked between DN2/3 and DN4 stages
• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice
|
|
• T cell numbers in the spleen are 40% to 50% of wild-type
|
|
• 5% to 10% of wild-type
|
|
• 40% of wild-type
|
|
• 5% to 10% of wild-type
|
|
• 5% to 10% of wild-type
|
|
• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice
|
|
• thymus contain 5% of the cells found in wild-type mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• 85% penetrance of thymic abnormalities
• CD3+ T cells are found distributed throughout the thymus
|
|
• increase in the percentage and number of double negative T cells
• increase in the percentage of cells in the DN3 stage
|
|
• the boundary between the cortical and medullary regions is not well defined
|
|
• progressive loss of thymic medullary cells
|
|
• substantial reduction in the number of thymocytes
|
|
• partial block in the transition from double negative to double positive T cells
• block appears to be at the DN3 to DN4 transition as a significant proportion of cells fail to down regulate CD25
|
|
• slight decrease in the percentage and number of T cells in the spleen and lymph nodes but not in the intestine
|
|
• thymocytes lack Mg2+ inhibitable current
• however, Mg2+ influx into freshly isolated T cells is unaffected
|
|
• 85% penetrance of thymic abnormalities
• CD3+ T cells are found distributed throughout the thymus
|
|
• increase in the percentage and number of double negative T cells
• increase in the percentage of cells in the DN3 stage
|
|
• the boundary between the cortical and medullary regions is not well defined
|
|
• progressive loss of thymic medullary cells
|
|
• substantial reduction in the number of thymocytes
|
|
• partial block in the transition from double negative to double positive T cells
• block appears to be at the DN3 to DN4 transition as a significant proportion of cells fail to down regulate CD25
|
|
• slight decrease in the percentage and number of T cells in the spleen and lymph nodes but not in the intestine
|
|
• thymocytes lack Mg2+ inhibitable current
• however, Mg2+ influx into freshly isolated T cells is unaffected
|
|
• 85% penetrance of thymic abnormalities
• CD3+ T cells are found distributed throughout the thymus
|
|
• increase in the percentage and number of double negative T cells
• increase in the percentage of cells in the DN3 stage
|
|
• the boundary between the cortical and medullary regions is not well defined
|
|
• progressive loss of thymic medullary cells
|
|
• substantial reduction in the number of thymocytes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation
• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)
|
|
• total thymocyte numbers were not significantly different from wild-type
• in 3-5 weeks old mice the percentages of CD4+ and CD8+ single-positive thymocytes were reduced by 50-70%
• the numbers of CD4+ and CD8+ T lymphocytes in peripheral blood, spleen and lymph nodes were significantly reduced
|
|
• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation
• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)
|
|
• total thymocyte numbers were not significantly different from wild-type
• in 3-5 weeks old mice the percentages of CD4+ and CD8+ single-positive thymocytes were reduced by 50-70%
• the numbers of CD4+ and CD8+ T lymphocytes in peripheral blood, spleen and lymph nodes were significantly reduced
|
|
• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation
• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)
|
|
• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation
• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• homozygous mice that are also hemizygous or homozygous mice for Tg(Lck-cre)548Jxm, in which cre recombinase is active in T cell lineage, display: total thymic cellularity was reduced by 40-50%
• the number of mature CD4 positive and CD8 positive T lymphocytes in the spleen was reduced by 40-50%
• however, mutant has a normal CD4- positive and CD8-positive effector and memory T cell development
|
|
• after 1-2 days of culture, the number of cells in the double-positive compartment were dramatically reduced
|
|
• homozygous mice that are also hemizygous or homozygous mice for Tg(Lck-cre)548Jxm, in which cre recombinase is active in T cell lineage, display: total thymic cellularity was reduced by 40-50%
• the number of mature CD4 positive and CD8 positive T lymphocytes in the spleen was reduced by 40-50%
• however, mutant has a normal CD4- positive and CD8-positive effector and memory T cell development
|
|
• after 1-2 days of culture, the number of cells in the double-positive compartment were dramatically reduced
|
|
• homozygous mice that are also hemizygous or homozygous mice for Tg(Lck-cre)548Jxm, in which cre recombinase is active in T cell lineage, display: total thymic cellularity was reduced by 40-50%
• the number of mature CD4 positive and CD8 positive T lymphocytes in the spleen was reduced by 40-50%
• however, mutant has a normal CD4- positive and CD8-positive effector and memory T cell development
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mice are viable; strong variation in percentage of RFP-positive T lymphocytes is observed between individual mice, as well as activation in non-T lineage cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Lung and salivary gland inflammation in Tgfb1tm2.1Doe/Tgfb1tm2.1Doe Tg(Lck-cre)548Jxm/? (b,e) and Tgfb1tm1Doe/Tgfb1tm2.1Doe Tg(Lck-cre)548Jxm/? (c,f) mice
|
• there is more than a 10% reduction in mean weight of mice compared to controls at 5 months of age
|
|
• modest salivary gland inflammation is observed in mice at 5 months of age
|
|
• there are slightly more CD8+ T cells than CD4+ T cells among splenocytes
|
|
• there is a higher percentage of T cells expressing activation markers
|
|
• thymus weight at 5 months of age is reduced by more than a third
|
|
• thymus cellularity is reduced by more than two-thirds at 1 month of age
|
|
• spleen weight is reduced by about a third at 2 months of age
|
|
• spleen cellularity is about half that of controls at 5 months of age
|
|
• interferon-gamma secretion is enhanced in mutant T cells when activated with various stimuli
|
|
• interleukin-2 secretion is enhanced in mutant T cells when activated with various stimuli
|
|
• modest salivary gland inflammation is observed in mice at 5 months of age
|
|
• modest inflammation is observed in the lungs at 5 months of age
|
|
• modest inflammation is observed in the lungs at 5 months of age
|
|
• modest salivary gland inflammation is observed in mice at 5 months of age
|
|
• thymus weight at 5 months of age is reduced by more than a third
|
|
• thymus cellularity is reduced by more than two-thirds at 1 month of age
|
|
• thymus weight at 5 months of age is reduced by more than a third
|
|
• thymus cellularity is reduced by more than two-thirds at 1 month of age
|
|
• there are slightly more CD8+ T cells than CD4+ T cells among splenocytes
|
|
• spleen weight is reduced by about a third at 2 months of age
|
|
• spleen cellularity is about half that of controls at 5 months of age
|
|
• there is a higher percentage of T cells expressing activation markers
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mice have increased mortality following treatment with a CD3epsilon antibody
• mortality increases following treatment with staphylococcal enterotoxin A (SEA)
• however, treatment with CD3epsilon antibody or staphylococcal enterotoxin A (SEA) and a COX-2 inhibitor reverses the increase in mortality
|
|
• treatment with a CD3epsilon antibody leads to inflammation of the cecum
|
|
• 2 hours after CD3epsilon antibody treatment, interferon-gamma levels are increased
|
|
• 8 hours after CD3epsilon antibody treatment, IL-6 levels are increased
|
|
• 2 hours after CD3epsilon antibody treatment, TNF-alpha levels are increased
|
|
• mortality increases following treatment with staphylococcal enterotoxin A (SEA)
|
|
• treatment with a CD3epsilon antibody leads to increased gastrointestinal damage in the cecum including mucosal and submucosal edema and inflammation along with necrotic regions that are denuded of mucosa
|
|
• treatment with a CD3epsilon antibody leads to inflammation of the cecum
|
|
• 2 hours after CD3epsilon antibody treatment, interferon-gamma levels are increased
|
|
• 8 hours after CD3epsilon antibody treatment, IL-6 levels are increased
|
|
• 2 hours after CD3epsilon antibody treatment, TNF-alpha levels are increased
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• in the peripheral blood
|
|
• mice exhibit T cell lymphopenia
• however, chemical depletion of macrophages ameliorates T cell lymphopenia
|
|
• CD4+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice
• however, thymic CD4+ counts are normal
|
|
• CD8+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice
• however, thymic CD8+ counts are normal
|
|
• in the peripheral blood
|
|
• mice exhibit T cell lymphopenia
• however, chemical depletion of macrophages ameliorates T cell lymphopenia
|
|
• CD4+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice
• however, thymic CD4+ counts are normal
|
|
• CD8+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice
• however, thymic CD8+ counts are normal
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mutant single positive thymocytes are more sensitive to apoptosis than wild-type
|
|
• percentage of T cells in peripheral lymphoid organs is significantly lower than in controls
|
|
• mature CD4+ T cells are reduced in number compared to controls
|
|
• mature CD8+ T cells are reduced in number compared to controls
|
|
• mutant single positive thymocytes are more sensitive to apoptosis than wild-type
|
|
• percentage of T cells in peripheral lymphoid organs is significantly lower than in controls
|
|
• mature CD4+ T cells are reduced in number compared to controls
|
|
• mature CD8+ T cells are reduced in number compared to controls
|
|
• mutant single positive thymocytes are more sensitive to apoptosis than wild-type
|
|
• mutant single positive thymocytes are more sensitive to apoptosis than wild-type
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• T cells stimulated with anti-CD3 antibodies exhibit impaired autophagy compared with wild-type cells
|
|
• in the spleen
|
|
• of T cells stimulated with anti-CD3 antibodies
|
|
• T lymphocytes exhibit increased mitochondria and endoplasmic reticulum content compared with wild-type cells
|
|
• in the spleen
|
|
• in the spleen
|
|
• T cells stimulated with anti-CD3 antibodies exhibit impaired autophagy compared with wild-type cells
|
|
• in the spleen
|
|
• of T cells stimulated with anti-CD3 antibodies
|
|
• T cells exhibit overproduction of reactive oxygen species compared with wild-type cells
|
|
• in the spleen
|
|
• of T cells stimulated with anti-CD3 antibodies
|
|
• T lymphocytes exhibit increased mitochondria and endoplasmic reticulum content compared with wild-type cells
|
|
• in the spleen
|
|
• in the spleen
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• T cell populations in thymus, spleen and blood are similar to controls as assessed by FACS analysis
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mice are viable; strong variation in percentage of RFP-positive T lymphocytes is observed between individual mice, as well as activation in non-T lineage cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice
• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection
|
|
• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology
|
|
• significant decrease in SP cell numbers
|
|
• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice
• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection
|
|
• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology
|
|
• significant decrease in SP cell numbers
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• at 6 months of age
|
|
• increased number of activated CD4 cells at 6 months of age
|
|
• increased production of IL-2, IL-10, and IFNG
|
|
• high titers of dsDNA antibodies are present at 6 months of age
|
|
• at 6 months of age
|
|
• increased number of activated CD4 cells at 6 months of age
|
|
• increased production of IL-2, IL-10, and IFNG
|
|
• at 6 months of age
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• developmental block specifically occurs at the double negative (DN) stage
• decreased percentage of TCR betahiCD3hi mature thymocytes as well as decreased cell numbers are observed
|
|
• percentage of cells undergoing positive selection is decreased by ~60% compared to controls
• significant decrease in mature SP cell numbers
|
|
• numbers of double negative (DN) cells are comparable to controls; however higher percentage of DN cells result
|
|
• a significant decrease in unfractionated double-positive thymocyte numbers compared to Tg-negative littermates; 70% decrease is observed accompanying the developmental block
|
|
• significant decrease in numbers
|
|
• significant decrease in numbers
|
|
• numbers of CD3+, Cd4+, and CD8+ T cells are decreased by ~50-60% in spleen and lymph nodes
|
|
• antigen-specific T cell activation is decreased, with decreased proliferation in response to antigen observed
|
|
• developmental block specifically occurs at the double negative (DN) stage
• decreased percentage of TCR betahiCD3hi mature thymocytes as well as decreased cell numbers are observed
|
|
• percentage of cells undergoing positive selection is decreased by ~60% compared to controls
• significant decrease in mature SP cell numbers
|
|
• numbers of double negative (DN) cells are comparable to controls; however higher percentage of DN cells result
|
|
• a significant decrease in unfractionated double-positive thymocyte numbers compared to Tg-negative littermates; 70% decrease is observed accompanying the developmental block
|
|
• significant decrease in numbers
|
|
• significant decrease in numbers
|
|
• numbers of CD3+, Cd4+, and CD8+ T cells are decreased by ~50-60% in spleen and lymph nodes
|
|
• antigen-specific T cell activation is decreased, with decreased proliferation in response to antigen observed
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• CD3+B220+ double positive cells accumulate
|
|
• CD3+B220+ double positive cells accumulate
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• in CD8+ T cells upon stimulation with anti-CD3 and anti-CD28 antibodies
|
|
• partial at the DN3 to DN4 stage
|
|
• in the thymus and lymph node
|
|
• absolute numbers
|
|
• general reduction in T cells
• more prominent reduction in CD8+ T cells compared with CD4+ T cells
|
|
• reduced regulatory CD4+ T cells in the spleen
|
|
• absolute numbers
|
|
• reduced cytotoxic CD8+ T cells in the spleen
|
|
• absolute numbers
|
|
• reduced regulatory CD4+ T cells in the spleen
• reduced CD3+ T cells
|
|
• most prominent in young mice
|
|
• in CD8+ T cells upon stimulation with anti-CD3 and anti-CD28 antibodies
|
|
• partial at the DN3 to DN4 stage
|
|
• in the thymus and lymph node
|
|
• absolute numbers
|
|
• general reduction in T cells
• more prominent reduction in CD8+ T cells compared with CD4+ T cells
|
|
• reduced regulatory CD4+ T cells in the spleen
|
|
• absolute numbers
|
|
• reduced cytotoxic CD8+ T cells in the spleen
|
|
• absolute numbers
|
|
• reduced regulatory CD4+ T cells in the spleen
• reduced CD3+ T cells
|
|
• most prominent in young mice
|
|
• in CD8+ T cells upon stimulation with anti-CD3 and anti-CD28 antibodies
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• CD4+ T cell numbers are reduced by about half in the spleen and lymph nodes
|
|
• CD8+ T cell numbers in the spleen and lymph node are only 66% of that found in wild-type
|
|
• spleen size is significantly reduced mostly due to a reduction in the number of T cells
|
|
• when transferred into a immunocompetent syngeneic host, mutant T cells fail to survive where as wild-type T cells do
|
|
• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts
• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells
• antigen driven proliferation is normal in these mice
|
|
• CD4+ T cell numbers are reduced by about half in the spleen and lymph nodes
|
|
• CD8+ T cell numbers in the spleen and lymph node are only 66% of that found in wild-type
|
|
• spleen size is significantly reduced mostly due to a reduction in the number of T cells
|
|
• when transferred into a immunocompetent syngeneic host, mutant T cells fail to survive where as wild-type T cells do
|
|
• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts
• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells
• antigen driven proliferation is normal in these mice
|
|
• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts
• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells
• antigen driven proliferation is normal in these mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment
|
|
|
• decreased thymus size in young adult (3-, 5- or 8-week-old) and older mice (6-month-old)
|
|
|
• decreased thymus weight in young adult and older mice
|
|
|
• reduced total thymocyte number in young adult and older mice
• however, development of double-negative (DN) thymocytes and CD4+ and CD8+ single-positive (SP) thymocytes is normal
• no significant change in DN1-4 thymic subsets
|
|
|
• decreased % of naive (CD44lowCD62Lhi) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)
|
|
|
• increased % of effector/memory T cells (CD44hiCD62Llo) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)
|
|
|
• decreased percentage of CD4+ and CD8+ single positive (SP) mature T lymphocytes in the spleen and lymph nodes of young adult (3-, 5- or 8-week-old) and older mice (6-month-old)
• decreased percentage of CD4+ and CD8+ T cells in peripheral blood
• however, expression of class I MHC molecules on CD4+ and CD8+ SP splenocytes is normal
|
|
|
• marked reduction in IL-2 and IFN-gamma production following stimulation of total splenocytes with anti-CD3 plus anti-CD28
|
|
|
• reduced T cell proliferation following stimulation of total splenocytes with anti-CD3 plus anti-CD28
|
|
|
• marked reduction in IFN-gamma production following stimulation of total splenocytes with anti-CD3 plus anti-CD28; also detected at the mRNA level
|
|
• marked reduction in IL-2 production following stimulation of total splenocytes with anti-CD3 plus anti-CD28; also detected at the mRNA level
|
|
|
• accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment
|
|
|
• decreased thymus size in young adult (3-, 5- or 8-week-old) and older mice (6-month-old)
|
|
|
• decreased thymus weight in young adult and older mice
|
|
|
• reduced total thymocyte number in young adult and older mice
• however, development of double-negative (DN) thymocytes and CD4+ and CD8+ single-positive (SP) thymocytes is normal
• no significant change in DN1-4 thymic subsets
|
|
|
• decreased % of naive (CD44lowCD62Lhi) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)
|
|
|
• increased % of effector/memory T cells (CD44hiCD62Llo) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)
|
|
|
• decreased percentage of CD4+ and CD8+ single positive (SP) mature T lymphocytes in the spleen and lymph nodes of young adult (3-, 5- or 8-week-old) and older mice (6-month-old)
• decreased percentage of CD4+ and CD8+ T cells in peripheral blood
• however, expression of class I MHC molecules on CD4+ and CD8+ SP splenocytes is normal
|
|
|
• marked reduction in IL-2 and IFN-gamma production following stimulation of total splenocytes with anti-CD3 plus anti-CD28
|
|
|
• reduced T cell proliferation following stimulation of total splenocytes with anti-CD3 plus anti-CD28
|
|
|
• accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment
|
|
|
• reduced T cell proliferation following stimulation of total splenocytes with anti-CD3 plus anti-CD28
|
|
|
• accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment
|
|
|
• decreased thymus size in young adult (3-, 5- or 8-week-old) and older mice (6-month-old)
|
|
|
• decreased thymus weight in young adult and older mice
|
|
|
• reduced total thymocyte number in young adult and older mice
• however, development of double-negative (DN) thymocytes and CD4+ and CD8+ single-positive (SP) thymocytes is normal
• no significant change in DN1-4 thymic subsets
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mice are viable; strong variation in percentage of RFP-positive T lymphocytes is observed between individual mice, as well as activation in non-T lineage cells
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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