Analysis Tools|
Allele Symbol Allele Name Allele ID |
Foxp3+ wild type MGI:2436058 |
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| Summary |
31 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• viable and fertile with no signs of lymphoproliferative disorders
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• females are phenotypically normal and indistinguishable from wild-type littermates
• EGFP expression in cells concordant with Foxp3 expression allows the kinetics of regulatory T cell proliferation and localization during a primary immune response
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• females develop and live normally, and are free of lymphoproliferative disease
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• mice have a 4-fold increase in numbers of late apoptotic cells among EGFP+ CD4+ single positive thymocytes relative to controls
• EGFP+ T cells show more apoptosis than EGFP- T cells from the same mouse, or EGFP+ and EGFP-T cells from controls
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• peripheral lymphoid tissues of females have fewer EGFP+ T cells than controls
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• females have a lower percentage of EGFP+ CD4+ single positive thymocytes in the periphery compared to controls
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• in response to stimulation, EGFP+ CD4+ T cells show substantial proliferation whereas cells from Foxp3tm2Tch females do not proliferate
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• mice have a 4-fold increase in numbers of late apoptotic cells among EGFP+ CD4+ single positive thymocytes relative to controls
• EGFP+ T cells show more apoptosis than EGFP- T cells from the same mouse, or EGFP+ and EGFP-T cells from controls
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• peripheral lymphoid tissues of females have fewer EGFP+ T cells than controls
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• females have a lower percentage of EGFP+ CD4+ single positive thymocytes in the periphery compared to controls
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• in response to stimulation, EGFP+ CD4+ T cells show substantial proliferation whereas cells from Foxp3tm2Tch females do not proliferate
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• mice have a 4-fold increase in numbers of late apoptotic cells among EGFP+ CD4+ single positive thymocytes relative to controls
• EGFP+ T cells show more apoptosis than EGFP- T cells from the same mouse, or EGFP+ and EGFP-T cells from controls
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• females have a lower percentage of EGFP+ CD4+ single positive thymocytes in the periphery compared to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• female mice heterozygous for this mutation appear normal in appearance and behavior and are fertile
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• marked decrease in T reg cells expressing the mutant allele in females
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• marked decrease in T reg cells expressing the mutant allele in females
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• females are fertile and phenotypically normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• heterozygotes are fertile, develop normally, and have normal lymphocyte development and function
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• by 6 weeks of age
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• by 6 weeks of age
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• by 6 weeks of age
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• by 6 weeks of age
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• by 6 weeks of age
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• by 6 weeks of age
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• the lethal inflammatory phenotype is partially rescued
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| N |
• regulatory T cells are able to suppress development of colitis in Rag1 null mice receiving naive T cells
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• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice
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• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice
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• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice
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• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1tm1Flv/Tgfb1tm2Flv Tg(Cd4-cre)1Cwi mice
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• in the peripheral and mesenteric lymph nodes, but not the spleen
• 2-fold increase in mesenteric regulatory T cell
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N |
• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1tm1Flv/Tgfb1tm2Flv Tg(Cd4-cre)1Cwi mice
• mice exhibit normal susceptibility to experimental autoimmune encephalomyelitis and Th17 cell differentiation
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• in the peripheral and mesenteric lymph nodes, but not the spleen
• 2-fold increase in mesenteric regulatory T cell
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1tm1Flv/Tgfb1tm2Flv Tg(Cd4-cre)1Cwi mice
• mice exhibit normal susceptibility to experimental autoimmune encephalomyelitis and Th17 cell differentiation
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• in the peripheral and mesenteric lymph nodes, but not the spleen
• 2-fold increase in mesenteric regulatory T cell
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N |
• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1tm1Flv/Tgfb1tm2Flv Tg(Cd4-cre)1Cwi mice
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• in the peripheral and mesenteric lymph nodes, but not the spleen
• 2-fold increase in mesenteric regulatory T cell
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• moribund within 35 days of birth
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• in the spleen and peripheral lymph node
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• in the spleen and peripheral lymph nodes
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• in the spleen and peripheral lymph nodes
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• in peripheral lymph node at day 20
• however, thymic and splenic regulatory T cell numbers are normal at day 12
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• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells
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• infiltrate of leukocytes in the salivary glands, lung interstitia, liver sinusoids, pancreas acini, stomach and colon mucosa
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• lack of mobility associated with hunched posture
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• crusting of the ears, eyelids and tail
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• scurfy tail
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• in the spleen and peripheral lymph node
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• in the spleen and peripheral lymph nodes
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• in the spleen and peripheral lymph nodes
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• in peripheral lymph node at day 20
• however, thymic and splenic regulatory T cell numbers are normal at day 12
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• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• the lethal inflammatory phenotype is completely rescued
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| N |
• the lethal inflammatory phenotype is completely rescued
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• Treg cells are not properly maintained
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• Treg cells are not properly maintained
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• regulatory T cells are not properly maintained
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• slightly attenuated suppressive activity
• in a transfer model of colitis, regulatory T cells fail to inhibit wasting disease and colitis compared with wild-type cells
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• regulatory T cells are not properly maintained
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• slightly attenuated suppressive activity
• in a transfer model of colitis, regulatory T cells fail to inhibit wasting disease and colitis compared with wild-type cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal regulatory T cell differentiation and homeostasis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice mount CD4+ T cell responses in response to immunization with OVA and LPS that are indistinguishable from wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• from 6 weeks of age
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| N |
• mice exhibit normal response to experimental autoimmune encephalomyelitis
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• from 6 weeks of age, mice exhibit a lymphoproliferative disorder unlike wild-type mice
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• increased when induced by regulatory T cells
• however, anti-IL4 antibody abolishes Th2 instruction
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• slightly decreased proportion in the spleen
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• in splenic red pulp
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• in the bronchoalveolar lavage fluid of ovalbumin-treated mice
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• absolute numbers
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• absolute numbers
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• slightly
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• modestly
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• ovalbumin-specific IgE in ovalbumin-treated mice
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• CD4+ T cells and anti-CD3-stimulated splenocytes produce more Th2 cytokines (IL4, IL5 and IL10) compared with wild-type mice
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• when injected into ovalbumin-immunized wild-type mice, regulatory T cells exacerbate Th2 immune responses in allergic reaction compared with wild-type cells
• however, regulatory T cells exhibit normal suppressive functions and stability
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• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells
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• from 6 weeks of age, mice exhibit larger size and cellularity of peripheral lymphoid organs compared with wild-type mice
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• from CD4+ T cells
• from anti-CD3-stimulated splenocytes
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• from anti-CD3-stimulated splenocytes
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• from CD4+ T cells in the lungs
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• from CD4+ T cells
• from anti-CD3-stimulated splenocytes
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• from CD4+ T cells
• from anti-CD3-stimulated splenocytes
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• massive infiltration in the lungs, stomach, skin and liver
• however, no lesions are observed in the kidney and colon
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• from 6 weeks of age
• more severe when induced by ovalbumin with increased total cells and eosinophils in the bronchoalveolar lavage fluid
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• severe at 16 weeks
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• severe at 16 weeks
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• from 6 weeks of age
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• from 6 weeks of age
• more severe when induced by ovalbumin with increased total cells and eosinophils in the bronchoalveolar lavage fluid
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• from 6 weeks of age
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• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells
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• from 6 weeks of age, mice exhibit a lymphoproliferative disorder unlike wild-type mice
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• increased when induced by regulatory T cells
• however, anti-IL4 antibody abolishes Th2 instruction
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• slightly decreased proportion in the spleen
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• in splenic red pulp
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• in the bronchoalveolar lavage fluid of ovalbumin-treated mice
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• absolute numbers
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• absolute numbers
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• slightly
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• modestly
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• ovalbumin-specific IgE in ovalbumin-treated mice
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• CD4+ T cells and anti-CD3-stimulated splenocytes produce more Th2 cytokines (IL4, IL5 and IL10) compared with wild-type mice
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• when injected into ovalbumin-immunized wild-type mice, regulatory T cells exacerbate Th2 immune responses in allergic reaction compared with wild-type cells
• however, regulatory T cells exhibit normal suppressive functions and stability
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• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• regulatory T cells exhibit normal suppressive functions and stability
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• severe
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• the ratio of YFP+/YFP- regulatory T cells is normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Skin inflammation and enlarged lymph nodes and spleen in Cd28tm1Ltu/Cd28tm1Ltu Foxp3tm4(YFP/icre)Ayr/Foxp3+ mice
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• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes
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• at 2 to 5 months with accumulation of activated T cells
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• in the lymph node
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• in the thymus but not lymph node or spleens
• in the thymus and periphery of female mice at 3 months
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• in the lymph nodes and spleen at 2 to 5 months
• with IFN-gamma expression
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• at 2 to 5 months with accumulation of activated T cells
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• by 8 to 12 weeks with crusting eyelids, facial hair loss, which progressed to hair loss on the trunk, skin lesions, an ill appearance, ruffled fur, hunching and reduced movements
• however, CD28-sufficient regulatory T cells prevent the autoimmune disease development
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• 20 days after immunization, mice treated with rMOG/CFA plus pertussis toxin exhibit increased clinical score and fail to recover compared with wild-type mice
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• at 2 to 5 months
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• at 2 to 5 months
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• facial hair loss, which progressed to hair loss on the trunk by 8 to 12 weeks
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• by 8 to 12 weeks
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• by 8 to 12 weeks
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• by 8 to 12 weeks
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• by 8 to 12 weeks
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• crusting by 8 to 12 weeks
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• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes
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• at 2 to 5 months with accumulation of activated T cells
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• in the lymph node
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• in the thymus but not lymph node or spleens
• in the thymus and periphery of female mice at 3 months
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• in the lymph nodes and spleen at 2 to 5 months
• with IFN-gamma expression
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• at 2 to 5 months with accumulation of activated T cells
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• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• injected B16F10 tumor model in tamoxifen-treated mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• shorter than normal lifespans
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• crusting ears
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• increased CD4/CD8 positive in the spleen
• increased frequencies of CD44high increased CD62Llow effector/memory T cells with reduced CD44low CD62Lhigh
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• increased Th1, Th2, and Th17 responses.
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• reduced Tregs suppressive function
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• significant Th2 (allergic) responses in the lung and spleen with increased collagen deposits
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• from CD4+ and CD8+ T cells
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• crusting eyelids
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• crusting ears
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• crusting eyelids
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• increased CD4/CD8 positive in the spleen
• increased frequencies of CD44high increased CD62Llow effector/memory T cells with reduced CD44low CD62Lhigh
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• increased Th1, Th2, and Th17 responses.
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• reduced Tregs suppressive function
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• tamoxifen-treated mice challenged with sheep red blood cell (sRBC) immunization show altered ratios of naive and effector/central memory CD4+ and CD8+ T cells in the spleen
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• tamoxifen-treated mice challenged with sheep red blood cell (sRBC) immunization show increased CD4+ T cell numbers in the spleen
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• tamoxifen-treated mice challenged with sRBC or OVA/Alum immunization show an increase in T follicular cells
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• tamoxifen-treated mice challenged with sRBC or OVA/Alum immunization show expanded germinal center (increased GL7/B220+ B cells)
• while GCs are expanded, they show normal gross morphology with no increase in follicle size but more GL7+ area per follicle
• however, mice do not show increased GC reactions after a booster immunization of sRBCs
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• tamoxifen-treated mice challenged with sheep red blood cell (sRBC) immunization show altered adaptive response after immunization, with more T follicular (Tfol) cells but no change in the ratio of T follicular helper (Tfh) to T follicular regulatory (Tfr) cells, expanded germinal centers (GCs; increased GL7/B220+ B cells) and higher titers of serum anti-sRBC IgG
• tamoxifen-treated mice challenged with sRBC immunization show increased numbers of CD4+ T cells and altered ratios of nave and effector/central memory CD4+ and CD8+ T cells in the spleen
• tamoxifen-treated mice challenged with OVA/Alum show increased Tfol cells and expanded GCs
• tamoxifen-treated mice still show increased Tfol cells and CD4+ T cells, altered ratios of CD4+/CD8+ subtypes, and increased IFN-gamma expression in CD4+/CD8+ T cells after a booster immunization of sRBCs
• however, splenic Treg level is not altered in tamoxifen-treated mice challenged with sRBC
• however, mice do not show increased GC reactions after a booster immunization of sRBCs, with titer or serum anti-sRBC IgG no different from controls and no higher than those seen after a single immunization
• administration of Anakinra (IL-1RA) reverses the increased responses
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• tamoxifen-treated mice challenged with sRBC immunization show higher titers of serum anti-sRBC IgG
• however, mice show no differences in titer of serum anti-sRBC IgG after a booster immunization of sRBCs from controls and no higher levels than those seen after a single immunization
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• tamoxifen-treated mice challenged with sheep red blood cell (sRBC) immunization show altered ratios of naive and effector/central memory CD4+ and CD8+ T cells in the spleen
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• tamoxifen-treated mice challenged with sheep red blood cell (sRBC) immunization show increased CD4+ T cell numbers in the spleen
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• tamoxifen-treated mice challenged with sRBC or OVA/Alum immunization show an increase in T follicular cells
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• tamoxifen-treated mice challenged with sRBC or OVA/Alum immunization show expanded germinal center (increased GL7/B220+ B cells)
• while GCs are expanded, they show normal gross morphology with no increase in follicle size but more GL7+ area per follicle
• however, mice do not show increased GC reactions after a booster immunization of sRBCs
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• tamoxifen-treated mice challenged with sRBC immunization show higher titers of serum anti-sRBC IgG
• however, mice show no differences in titer of serum anti-sRBC IgG after a booster immunization of sRBCs from controls and no higher levels than those seen after a single immunization
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• contrary to germ line deletion, mice are born in Mendelian ratios with no sex bias
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| N |
• mice exhibit normal frequencies and numbers of B and T cells and activated T cells
• unchallenged mice exhibit no heightened inflammatory response and normal T regulator cell suppressor function
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• flu-infected mice exhibit a greater decrease in average blood oxygen saturation, overall lung tissue damage and decreased surface temperature compared with wild-type mice
• however, flu-infected mice exhibit normal T cell activation and T regulatory cell mediated suppression of anti-vial immunity
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• flu-infected mice exhibit a greater decrease in average blood oxygen saturation compared with wild-type mice
• however, mice do not exhibit anemia
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• in flu-infected mice
• however, core body temperature is normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increased frequency at 2 months that may be due to increased thymic output
• further increase at 6 months as in Bcl2l11tm1.1Ast homozygotes
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• increased frequency at 2 months that may be due to increased thymic output
• further increase at 6 months as in Bcl2l11tm1.1Ast homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• Foxp3+ regulatory T cells are increased 2-fold relative to wild-type mice
• the numbers of regulatory T cells is increased in the peripheral and mesenteric lymph nodes, spleens and among the lamina propria mononuclear cell infiltrate of the colon
• proliferation of Foxp3+ cells and CD4+CD8+Foxp3- cells is increased in association with the development of colitis
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• Foxp3+ regulatory T cells are increased 2-fold relative to wild-type mice
• the numbers of regulatory T cells is increased in the peripheral and mesenteric lymph nodes, spleens and among the lamina propria mononuclear cell infiltrate of the colon
• proliferation of Foxp3+ cells and CD4+CD8+Foxp3- cells is increased in association with the development of colitis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• reduced suppression of wild-type naive CD4+ T cells when compared with wild-type Treg cells
• TGFbeta induced Foxp3-expressing Treg, iTreg, cells have a reduced ability to inhibit the proliferation of nave CD4+ T cells
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• reduced suppression of wild-type naive CD4+ T cells when compared with wild-type Treg cells
• TGFbeta induced Foxp3-expressing Treg, iTreg, cells have a reduced ability to inhibit the proliferation of nave CD4+ T cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in mice treated with diphtheria toxin and MOG
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 03/25/2025 MGI 6.24 |
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