Areg^{tm2c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Areg^{tm2c(EUCOMM)Hmgu}
• Name: amphiregulin; targeted mutation 2c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5806473
• Synonyms: Areg^Fl
• Gene: Areg Location: Chr5:91287458-91296291 bp, + strand Genetic Position: Chr5, 44.78 cM
• Alliance: Areg^{tm2c(EUCOMM)Hmgu} page
• IMPC: Areg gene page

Mutation origin

• Mutant Cell Line: HEPD0772_4_A08
• Germline Transmission: Earliest citation of germline transmission: J:224904
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 91290869 of Chromosome 5 upstream of the critical exon 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 91292610. The critical exon 3 and 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:224904)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Areg Mutation: 29 strains or lines available

References

• Original: J:224904 Arpaia N, et al., A Distinct Function of Regulatory T Cells in Tissue Protection. Cell. 2015 Aug 27;162(5):1078-89
• All: 4 reference(s)