Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hprt1tm1(CAG-Gys1*)Jjg mutation
(0 available);
any
Hprt1 mutation
(1274 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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homeostasis/metabolism
cellular
nervous system
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• extensive brain accumulation of glycogen
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk1tm1.2Kuta mutation
(0 available);
any
Mapk1 mutation
(40 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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behavior/neurological
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• context freezing response is significantly reduced
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• cued fear conditioning response is reduced after a 48 h retention delay
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• resident males show a shorter latency to attack and attack for a longer duration with a higher frequency
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• spend more time in the center of an open field and spend more time in the open arms of a plus maze compared to controls
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• fail to show a preference for a novel mouse
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• decrease in interaction with a novel object compared to controls
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• pups are more scattered in the home cage
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• show a significantly shorter duration of crouching to keep the pups warm and to nurse them
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• show little nest building activity and build shallower nest compared to controls
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• males display persistent interest when presented with the same female indicating a failure to develop social memory
• fail to show a preference for a novel mouse
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• in an open field test for social versus inanimate preference, mice show no significant difference in time spent interacting with the social or inanimate targets unlike controls
• in a sociability test for social versus empty preference, mice show a significant decrease in interaction with the social target compared to controls
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nervous system
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• in the cortices at 13 weeks of age
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taste/olfaction
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N |
• no defect in olfaction is detected
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Abl1tm1Ajk mutation
(1 available);
any
Abl1 mutation
(92 available)
Abl2tm1Ajk mutation
(0 available);
any
Abl2 mutation
(79 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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mortality/aging
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N |
• only 35% of the expected Mendelian numbers are born
• those that were born survived well into adulthood
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nervous system
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• smaller dendrite arbors resulting in more densely packed neurons in all layers of cortex in 6-8 weeks old mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Huwe1tm1Alas mutation
(0 available);
any
Huwe1 mutation
(63 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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mortality/aging
nervous system
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• at E13.5, E15.5, E18.5, fewer progenitors exit the cell cycle compared with wild-type mice
• at E18.5, proliferation of neuronal precursors is increased compared to in wild-type mice
• at E18.5, the length of the cycle is lengthened compared to in wild-type cells
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• brains contain ectopic cellular clusters in differentiated striatal regions unlike in wild-type mice
• however, no abnormalities are observed at E15.5
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• the cortex exhibits an increase in cellular density and decrease in intervening neuropil compared to in wild-type mice
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• laminar organization is altered with no clearly identifiable superficial neuron layers compared to in wild-type mice
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cellular
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• at E13.5, E15.5, E18.5, fewer progenitors exit the cell cycle compared with wild-type mice
• at E18.5, proliferation of neuronal precursors is increased compared to in wild-type mice
• at E18.5, the length of the cycle is lengthened compared to in wild-type cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dmrta2tm1.1Fuma mutation
(0 available);
any
Dmrta2 mutation
(15 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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nervous system
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N |
• mice exhibit normal formation of the cortical hem and the hippocampal primordium in the medial cortex
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• moderate at E15.5
• however, size is normal at E12.5
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spentm2.1Hon mutation
(3 available);
any
Spen mutation
(140 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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mortality/aging
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• increased mortality before 10 weeks of age
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growth/size/body
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• although normal at birth, mice are growth retarded at 3 weeks of age
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nervous system
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• severe reductions in the thickness of the cerebral cortex
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sqstm1tm1.1Ewa mutation
(0 available);
any
Sqstm1 mutation
(32 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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growth/size/body
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• body weight gradually increases after 20 weeks of age, becoming significantly greater than littermate controls by 27 weeks of age
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adipose tissue
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• increase in visceral fat
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behavior/neurological
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gys1tm1c(EUCOMM)Wtsi mutation
(0 available);
any
Gys1 mutation
(56 available)
Nhlrc1tm1(KOMP)Vlcg mutation
(0 available);
any
Nhlrc1 mutation
(14 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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nervous system
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• reduced induction of seizures by Kainic acid
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• modest seizure response following train stimulation of CA3-CA1 synapse
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• modest neurodegeneration in brains
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cellular
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• modest level of autophagy
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behavior/neurological
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• reduced induction of seizures by Kainic acid
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• modest seizure response following train stimulation of CA3-CA1 synapse
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gys1tm1c(EUCOMM)Wtsi mutation
(0 available);
any
Gys1 mutation
(56 available)
Nhlrc1tm1(KOMP)Vlcg mutation
(0 available);
any
Nhlrc1 mutation
(14 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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nervous system
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N |
• no neurodegeneration in brains
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• reduced induction of seizures by Kainic acid
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• modest seizure response following train stimulation of CA3-CA1 synapse
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cellular
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N |
• autophagy is not impaired
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behavior/neurological
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• reduced induction of seizures by Kainic acid
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• modest seizure response following train stimulation of CA3-CA1 synapse
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Nes-cre)1Kag mutation
(0 available)
Wasltm1.1Ttha mutation
(0 available);
any
Wasl mutation
(39 available)
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mortality/aging
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• mice are born phenotypically normal with a near-Mendelian incidence (30% vs. 25% expected); however, all mice die prior to weaning within 3 to 4 weeks after birth (P18-P24)
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growth/size/body
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• at P7, P10 and P13, mice show a significant reduction in average body weight relative to controls
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• by P5-P7, mice exhibit noticeably retarded growth rates relative to controls
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behavior/neurological
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• at P15, mice exhibit locomotor disturbance with lethargic pace
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• at P15, mice exhibit broad-base stance/gait pattern
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nervous system
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• at P15, mice show a marked decrease in brain vascularization relative to controls
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• at P15, an unusually large amount of cerebrospinal fluid (CSF) is drained out from the mutant skull during brain isolation
• CSF accumulation in the skull results in brain swelling and enlarged brain hemispheres
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• hydrocephalus is likely caused by a blocked connection between the third and fourth ventricles
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• the rostral segment of the aqueduct of Sylvius is abnormally narrow or completely collapsed
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• at P15, mutant brains are noticeably larger than control brains
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• hydrocephalic mice show major defects in the ependymal layer accompanied by a severe denudation of ependymal layers lining the ventricular wall
• N-cadherin immune-staining confirmed defects in ependymal layer integrity
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• at P14-18, immunostaining with anti-acetylated tubulin revealed a severe reduction or absence of ependymal cilia in the lateral ventricles
• SEM micrographs confirmed that the surface of ependymal cells lining the lateral ventricles is relatively smooth and lacks the numerous fine projections seen in controls
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• at 3 weeks of age, hydrocephalic mice show markedly enlarged ventricles in both anterior and posterior regions of lateral ventricles
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• in hydrocephalic mice, dilation of the fourth ventricle is observed
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• at 3 weeks of age, H&E-stained coronal brain sections revealed visibly dilated lateral ventricles along their entire rostrocaudal aspect
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• in hydrocephalic mice, the choroid plexus displays an undefined border with disrupted cell alignment between adjacent cells
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• an enlarged lumen is noted in the caudal part of the aqueduct
• in hydrocephalic mice, a unique type of columnar cells is absent in the rostral aqueduct wall due to aqueduct atresia and stenosis
• abnormal multilayered stratification of the ependymal cells lining along the caudal part of the aqueduct wall and increased cell numbers in the periaqueductal gray regions are observed
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• hydrocephalic mice show a profound reduction in the corpus callosum
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• hydrocephalic mice show a profound reduction in the caudate putamen
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• strikingly, hydrocephalic mice show absence of hippocampal formation
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• the spatial alignment and relation of cortical cell orientation are disrupted and compressed by the enlargement of the ventricle system
• however, no significant morphological deterioration is noted in anterior regions of the frontal cortex
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• hydrocephalic brains exhibit reduced thickness of the cerebral cortex along its rostral and caudal parts
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• hydrocephalic mice show a profound reduction in the cortical layers and septum
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• Ki-67 staining revealed a significant decrease in cell proliferation in the subventricular zone relative to control mice
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• GFAP staining revealed a marked increase in astrocyte density in the cerebral cortex and surrounding areas of the lateral ventricular regions, indicating astrogliosis
• mutant astrocytes tend to have an enlarged cell body and thicker processes than control astrocytes
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skeleton
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• at ~P10, mice develop a dome shaped skull morphology that becomes more prominent by P15
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• at P15, mice display abnormal curvature of the thoracic vertebrae
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• at P15, mice exhibit a hunchback body posture
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craniofacial
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• at ~P10, mice develop a dome shaped skull morphology that becomes more prominent by P15
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cardiovascular system
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• at P15, mice show a marked decrease in brain vascularization relative to controls
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cellular
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• at P14-18, immunostaining with anti-acetylated tubulin revealed a severe reduction or absence of ependymal cilia in the lateral ventricles
• SEM micrographs confirmed that the surface of ependymal cells lining the lateral ventricles is relatively smooth and lacks the numerous fine projections seen in controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Nes-cre)1Kag mutation
(0 available)
Wasltm1.1Ttha mutation
(0 available);
any
Wasl mutation
(39 available)
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normal phenotype
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• mice have a normal life span and display no obvious defects in external morphology, weight, reproductive vigor, or behavior relative to wild-type controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hif1atm1.1Stom mutation
(0 available);
any
Hif1a mutation
(48 available)
Hif1atm1Stom mutation
(0 available);
any
Hif1a mutation
(48 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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nervous system
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• density of blood vessels is reduced
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• thinner than in controls at E19
• dense nuclei found with a wavy architecture
• apoptotic neural and glial cells detected
• atrophy of the cerebral cortex in adults
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• significant reduction of neurons at 10 weeks of age although six layered structure of the cortex is maintained
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• 36% reduction of neurons at 10 weeks of age
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• 36% loss of neurons in the perirhinal cortex at 10 weeks of age
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• 12% reduction of neurons on the dorsal side
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behavior/neurological
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N |
• spontaneous activity is normal
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• significantly impaired memory consolidation after 4 days of testing using a radial maze task
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Setdb1tm1.1Yshk mutation
(1 available);
any
Setdb1 mutation
(56 available)
Tg(Nes-cre)1Kag mutation
(0 available)
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mortality/aging
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• born alive but do not survive beyond P10
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nervous system
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• increase in the number of astrocytes in the cortex at E18.5
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Analysis Tools