Gys1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gys1^{tm1c(EUCOMM)Wtsi}
• Name: glycogen synthase 1, muscle; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5619172
• Gene: Gys1 Location: Chr7:45084268-45106043 bp, + strand Genetic Position: Chr7, 29.31 cM
• Alliance: Gys1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Gys1 gene page

Mutation origin

• Mutant Cell Line: EPD0069_4_A05
• Germline Transmission: Earliest citation of germline transmission: J:218886
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 45091398 of Chromosome 7 upstream of the critical exons 6-8 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 6-8 at position 45093292. The critical exons 6-8 are thus flanked by loxP sites. Flp mediated recombination created the "conditional ready" (floxed). Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:173534, J:218886)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gys1 Mutation: 56 strains or lines available

References

• Original: J:218886 Duran J, et al., Impairment in long-term memory formation and learning-dependent synaptic plasticity in mice lacking glycogen synthase in the brain. J Cereb Blood Flow Metab. 2013 Apr;33(4):550-6
• All: 10 reference(s)