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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8206395
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: Anti-Isl1/2
Results
Specimen 1D Control: embryonic day 11.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - Pbx1 (assay MGI:8206352).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present 1D Control
Specimen 1D Pbx1MNdelta: embryonic day 11.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Pbx1 (assay MGI:8206352).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present 1D Pbx1MNdelta Expression was unaltered in the mutant.
Specimen 1D PbxMNdelta: embryonic day 11.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Pbx1 (assay MGI:8206352).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present 1D PbxMNdelta Expression was unaltered in the mutant.
Specimen 1E Control: embryonic day 11.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present 1E Control
Specimen 1E Pbx3-/-: embryonic day 11.5; Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Double labeled: green - Isl1 and Isl2; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord motor neuron Present 1E Pbx3-/- Expression was unaltered in the mutant.
Specimen 1E PbxMNdelta: embryonic day 11.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Og (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord motor neuron Present 1E PbxMNdelta Expression was unaltered in the mutant.
Specimen 2E Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Isl1 and Isl2; green - Smad1 (assay MGI:8207784) and Smad5 (assay MGI:8207824) (assay MGI:8207784) and Smad9 (assay MGI:8207825).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present 2E Control
Specimen 2E PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Isl1 and Isl2; green - Smad1 (assay MGI:8207784) and Smad5 (assay MGI:8207824) (assay MGI:8207784) and Smad9 (assay MGI:8207825).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present 2E PbxMNdelta Immunopositive motor neurons fail to migrate to a dorsolateral position in the mutant.
Specimen 2F Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Isl1 and Isl2; green - Nos1 (assay MGI:8206376).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present 2F Control
Specimen 2F PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Isl1 and Isl2; green - Nos1 (assay MGI:8206376).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present 2F PbxMNdelta Immunopositive motor neurons fail to migrate to a dorsolateral position in the mutant.
Specimen 7A brachial (Mecom): embryonic day 12.5 (more )
Note: Double labeled: red - Isl1 and Isl2; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present 7A Isl1/2 Mecom
Specimen 7A thoracic (Mecom): embryonic day 12.5 (more )
Note: Double labeled: red - Isl1 and Isl2; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present 7B Isl1/2 Mecom
Specimen 7C HoxCdelta/HoxA+/-: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10+, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Double labeled: red - Isl1 and Isl2; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present 7C HoxCdelta/HoxA+/- Isl1/2 Mecom
Specimen S1A brachial: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1 and Isl2; red - Pbx1 (assay MGI:8206352).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1A Pbx1 Isl1/2 brachial Expression was colocalized with Pbx1.
Specimen S1A thoracic: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1 and Isl2; red - Pbx1 (assay MGI:8206352).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S1A Pbx1 Isl1/2 thoracic Expression was colocalized with Pbx1.
Specimen S1B brachial: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1 and Isl2; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1B Pbx3 Isl1/2 brachial Expression was colocalized with Pbx3.
Specimen S1B thoracic: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1 and Isl2; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S1B Pbx3 Isl1/2 thoracic Expression was colocalized with Pbx3.
Specimen S1H Control left: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; green - Casp3 (assay MGI:8209239); blue - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H Control left
Specimen S1H PbxMNdelta left: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; green - Casp3 (assay MGI:8209239); blue - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H PbxMNdelta left
Specimen S1H Control right: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; green - Casp3 (assay MGI:8209239); blue - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H Control right
Specimen S1H PbxMNdelta right: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; green - Casp3 (assay MGI:8209239); blue - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H PbxMNdelta right
Specimen S1K Control left: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Isl1 and Isl2; red - Hoxc6 (assay MGI:8207396); green - Hoxc9 (assay MGI:8207397).
Structure Level Pattern Image Note
TS20: dorsal root ganglion Present S1K Control Hoxc6 Isl1/2 Hoxc9
Specimen S1K PbxMNdelta left: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Isl1 and Isl2; red - Hoxc6 (assay MGI:8207396); green - Hoxc9 (assay MGI:8207397).
Structure Level Pattern Image Note
TS20: dorsal root ganglion Present S1K PbxMNdelta Hoxc6 Isl1/2 Hoxc9
Specimen S1K Control right: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Isl1 and Isl2; red - Hoxa5 (assay MGI:8207399); green - Hoxc8 (assay MGI:8207401).
Structure Level Pattern Image Note
TS20: dorsal root ganglion Present S1K Control Hoxa5 Isl1/2 Hoxc8
Specimen S1K PbxMNdelta right: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Isl1 and Isl2; red - Hoxa5 (assay MGI:8207399); green - Hoxc8 (assay MGI:8207401).
Structure Level Pattern Image Note
TS20: dorsal root ganglion Present S1K PbxMNdelta Hoxa5 Isl1/2 Hoxc8
Specimen S2B Pbx3+/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3+ (more )
Note: Double labeled: red - Isl1 and Isl2; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S2B Isl1/2 Hb9 Pbx3+/- Expression co-localized with Mnx1 (Hb9) in hypaxial motor column neurons.
Specimen S2B Pbx3-/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Double labeled: red - Isl1 and Isl2; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S2B Isl1/2 Hb9 Pbx3-/- Expression was unaffected in hypaxial motor column neurons in Pbx3-/- mice.
Specimen S2C top: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3+ (more )
Note: Conditional mutant. Double labeled: red - Isl1 and Isl2; green - Nos1 (assay MGI:8206376).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present S2C nNos Isl1/2
Specimen S2C bottom: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3+ (more )
Note: Conditional mutant. Double labeled: red - Isl1 and Isl2; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present S2C Isl1/2 Foxp1
Specimen S2F1 Control rostral: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F1 rostral Control
Specimen S2F1 PbxMNdelta rostral: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F1 rostral PbxMNdelta
Specimen S2F2 Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F2 Control
Specimen S2F2 PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F2 PbxMNdelta
Specimen S2F3 Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F3 Control
Specimen S2F3 PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F3 PbxMNdelta
Specimen S2F4 Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F4 Control
Specimen S2F4 PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S2F4 PbxMNdelta
Specimen S2F5 Control caudal: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present S2F5 caudal Control
Specimen S2F5 PbxMNdelta caudal: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:8207834).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present S2F5 caudal PbxMNdelta
Specimen S4A1: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A1 Isl1/2 Hb9 Lhx3 , S4A1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4A2: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A2 Isl1/2 Hb9 , S4A2 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4A3: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A3 Isl1/2 Hb9 Lhx3 , S4A3 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4A4: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A4 Isl1/2 Hb9 Lhx3 , S4A4 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4B1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B1 Isl1/2 Hb9 , S4B1 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4B2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B2 Isl1/2 Hb9 , S4B2 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4B3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B3 Isl1/2 Hb9 Lhx3 , S4B3 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4B4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B4 Isl1/2 Hb9 Lhx3 , S4B4 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4C1: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C1 Isl1/2 Hb9 Lhx3 , S4C1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4C2: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C2 Isl1/2 Hb9 , S4C2 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4C3: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C3 Isl1/2 Hb9 , S4C3 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4C4: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C4 Isl1/2 Hb9 Lhx3 , S4C4 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4D1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D1 Isl1/2 Hb9 , S4D1 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4D2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D2 Isl1/2 Hb9 Lhx3 , S4D2 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4D3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D3 Isl1/2 Hb9 Lhx3 , S4D3 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4D4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D4 Isl1/2 Hb9 , S4D4 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4E thoracic Foxp1+/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1+ (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4E1 Isl1/2 Hb9 , S4E1 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
Specimen S4E thoracic Foxp1-/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1tm1Eem (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4E2 Isl1/2 Hb9 Lhx3 , S4E2 Isl1/2 Hb9 In the absence of Foxp1 the remaining hypaxial motor column neurons were clustered and well segregated.
Specimen S4E brachial Foxp1+/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1+ (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4E3 Isl1/2 Hb9 , S4E3 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
Specimen S4E brachial Foxp1-/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1tm1Eem (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4E4 Isl1/2 Hb9 Lhx3 , S4E4 Isl1/2 Hb9 In the absence of Foxp1 the remaining hypaxial motor column neurons were clustered and well segregated.
Specimen S4F thoracic HoxA/HoxC+/+: embryonic day 12.5 (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4F1 Isl1/2 Hb9 Lhx3 , S4F1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4F thoracic HoxA/HoxCdelta/delta: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10tm1Mrc, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4F2 Isl1/2 Hb9 Lhx3 , S4F2 Isl1/2 Hb9 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining hypaxial motor column and Isl1/2+ motor neurons was unaffected.
Specimen S4F brachial HoxA/HoxC+/+: embryonic day 12.5 (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4F3 Isl1/2 Hb9 , S4F3 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+), as well as a smaller group that expression Isl1/2 alone.
Specimen S4F brachial HoxA/HoxCdelta/delta: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10tm1Mrc, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Triple labeled: red - Isl1 and Isl2; blue - Lhx3 (assay MGI:8206372); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4F4 Isl1/2 Hb9 , S4F4 Isl1/2 Hb9 Lhx3 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining hypaxial motor column and Isl1/2+ motor neurons was unaffected.
Specimen SG Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Isl1 and Isl2; green - En1 (assay MGI:8210459).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord interneuron Present Regionally restricted S4G Control top Expression was in dorsal interneurons (dI3).
Specimen SG PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Isl1 and Isl2; green - En1 (assay MGI:8210459).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord interneuron Present Regionally restricted S4G PbxMNdelta top Expression was in dorsal interneurons (dI3).
Specimen S4H Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Isl1 and Isl2; red - RhD-retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4H Control center Expression was detected in hypaxial motor column neurons, which were co-stained with retrograde label.
Specimen S4H PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - RhD-retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4H PbxMNdelta center Expression was detected in hypaxial motor column neurons, which were co-stained with retrograde label. In the PbxMNdelta mice, the (Ils1/2+,Lhx3-) labeled neurons were dispersed within the spinal cord.
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03/18/2025
MGI 6.24 		The Jackson Laboratory