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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8206356
Gene symbol: Pbx3
Gene name: pre B cell leukemia homeobox 3
Antibody: Anti-Pbx3
Results
Specimen 1A: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: spinal cord central nervous system neuron Present Regionally restricted 1A Pbx3 Expression was detected in postmitotic spinal neurons.
TS20: spinal cord spinal cord motor neuron Present 1A Pbx3
Specimen 1C brachial (Foxp1): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted 1C Pbx3 Foxp1 Pbx3 is restricted to rostral brachial Foxp1+ lateral motor column neurons.
Specimen 1C brachial (Lhx3): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Absent 1C Pbx3 Lhx3 Expression was excluded from Lhx3+ medial motor column neurons.
TS20: spinal cord brachial region lateral motor column neuron Present 1C Pbx3 Lhx3
Specimen 1C thoracic (nNos): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Nos1 (assay MGI:8206376).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 1C Pbx3 nNos At thoracic levels, Pbx3 is expressed in hypaxial motor column and nNos+ preganglionic motor column neurons.
Specimen 1E Control: embryonic day 11.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Pbx3; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present 1E Control
Specimen 1E Pbx3-/-: embryonic day 11.5; Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Double labeled: red - Pbx3; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Absent 1E Pbx3-/-
Specimen 1E PbxMNdelta: embryonic day 11.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Og (more )
Note: Conditional mutant. Double labeled: red - Pbx3; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Absent 1E PbxMNdelta
Specimen S1B brachial: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1B Pbx3 Isl1/2 brachial Expression colocalized with Isl1/2 motor neurons.
Specimen S1B thoracic left: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S1B Pbx3 Isl1/2 thoracic Expression colocalized with Isl1/2 motor neurons.
Specimen S1B thoracic right: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S1B Pbx3 Lhx3 thoracic At thoracic levels Pbx3 is expressed in hypaxial motor column and preganglionic motor column neurons.
Specimen S1C (Hoxa5): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Hoxa5 (assay MGI:8207399).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted S1C Pbx3 Hoxa5 Expression in lateral motor column neurons in the rostral brachial region colocalized with Hoxa5.
Specimen S1C (Foxp1): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted S1C Pbx3 Foxp1 Expression in lateral motor column neurons in the rostral brachial region colocalized with Foxp1.
Specimen S1D (Hoxc8): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Hoxc8 (assay MGI:8207401).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted S1D Pbx3 Hoxc8 Expression in lateral motor column neurons in the caudal brachial region colocalized with a subset of Hoxc8 neurons.
Specimen S1D (Foxp1): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted S1D Pbx3 Foxp1 Expression in lateral motor column neurons in the caudal brachial region colocalized with a subset of Foxp1 neurons.
Specimen S1G Control rostral: embryonic day 12.5 (more )
Note: Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present S1G Control rostral
Specimen S1G Pbx1MNdelta rostral: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present S1G Pbx1MNdelta rostral
Specimen S1G Control middle: embryonic day 12.5 (more )
Note: Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present S1G Control
Specimen S1G Pbx1MNdelta middle: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present S1G Pbx1MNdelta
Specimen S1G Control caudal: embryonic day 12.5 (more )
Note: Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Weak S1G Control caudal Expression is normally absent or low in caudal brachial Hoxc8+ lateral motor column neurons.
TS20: spinal cord brachial region spinal cord medial motor column neuron Absent S1G Control caudal
Specimen S1G Pbx1MNdelta caudal: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: red - Pbx3; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present S1G Pbx1MNdelta caudal The Pbx3 expression domain was expanded in the Pbx1MNdelta mutants at caudal brachial levels.
TS20: spinal cord brachial region spinal cord medial motor column neuron Present S1G Pbx1MNdelta caudal The Pbx3 expression domain was expanded in the Pbx1MNdelta mutants at caudal brachial levels.
Specimen S2A Pbx3+/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3+ (more )
Structure Level Pattern Image Note
TS20: spinal cord brachial region Present S2A Pbx3 Pbx3+/-
Specimen S2A Pbx3-/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Structure Level Pattern Image Note
TS20: spinal cord brachial region Absent S2A Pbx3 Pbx3-/-
Specimen S2B Pbx3+/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3+ (more )
Note: Double labeled: red - Pbx3; green - Nos1 (assay MGI:8206376).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present S2B nNos Pbx3 Pbx3+/- , S2B Pbx3 Pbx3+/-
Specimen S2B Pbx3-/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Double labeled: red - Pbx3; green - Nos1 (assay MGI:8206376).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Absent S2B Pbx3 Pbx3-/- , S2B nNos Pbx3 Pbx3-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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