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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8207842
Gene symbol: Mnx1
Gene name: motor neuron and pancreas homeobox 1
Antibody: Anti-Hb9
Results
Specimen 4B1: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B1
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4B1 Expression was detected in hypaxial motor column neurons.
Specimen 4B2: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B2
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4B2 Expression was detected in hypaxial motor column neurons.
Specimen 4B3: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B3
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4B3 Expression was detected in hypaxial motor column neurons.
Specimen 4B4: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B4
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4B4 Expression was detected in hypaxial motor column neurons.
Specimen 4D1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D1 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
TS20: spinal cord thoracic region spinal cord motor neuron Present 4D1 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4D2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D2 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
TS20: spinal cord thoracic region spinal cord motor neuron Present 4D2 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4D3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D3 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
TS20: spinal cord thoracic region spinal cord motor neuron Present 4D3 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4D4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D4 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
TS20: spinal cord thoracic region spinal cord motor neuron Present 4D4 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4F1: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F1
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 4F1 Expression was detected in hypaxial motor column neurons.
Specimen 4F2: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F2
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 4F2 Expression was detected in hypaxial motor column neurons.
Specimen 4F3: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F3
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 4F3 Expression was detected in hypaxial motor column neurons.
Specimen 4F4: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F4
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 4F4 Expression was detected in hypaxial motor column neurons.
Specimen 4H1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H1 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
TS20: spinal cord brachial region spinal cord motor neuron Present 4H1 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
Specimen 4H2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H2 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
TS20: spinal cord brachial region spinal cord motor neuron Present 4H2 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
Specimen 4H3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H3 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
TS20: spinal cord brachial region spinal cord motor neuron Present 4H3 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
Specimen 4H4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: green - Mnx1; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H4 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
TS20: spinal cord brachial region spinal cord motor neuron Present 4H4 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Hb9(Mnx1)+ cells were intermixed with other motor neuron populations.
Specimen 7A brachial Hb9 (Mecom): embryonic day 12.5 (more )
Note: Double labeled: red - Mnx1; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7A Hb9 Mecom Expression was detected in medial motor column neurons and colocalized with Mecom+ neurons.
Specimen 7B thoracic Hb9 (Mecom): embryonic day 12.5 (more )
Note: Double labeled: red - Mnx1; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 7B Hb9 Mecom Expression was detected in medial motor column neurons and colocalized with Mecom+ neurons.
Specimen S1H Control left: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Mnx1; green - Casp3 (assay MGI:8209239); red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H Control left
Specimen S1H PbxMNdelta left: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Mnx1; green - Casp3 (assay MGI:8209239); red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H Control right
Specimen S1H Control right: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Mnx1; green - Casp3 (assay MGI:8209239); red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H PbxMNdelta left
Specimen S1H PbxMNdelta right: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Mnx1; green - Casp3 (assay MGI:8209239); red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present S1H PbxMNdelta right
Specimen S2B Pbx3+/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3+ (more )
Note: Double labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S2B Isl1/2 Hb9 Pbx3+/- Expression co-localized with Isl1/2 in hypaxial motor column neurons.
Specimen S2B Pbx3-/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Double labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S2B Isl1/2 Hb9 Pbx3-/- Expression was unaffected in hypaxial motor column neurons in the Pbx3-/- mice.
Specimen S4A1: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A1 Isl1/2 Hb9 Lhx3 , S4A1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4A2: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A2 Isl1/2 Hb9 , S4A2 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4A3: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A3 Isl1/2 Hb9 Lhx3 , S4A3 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4A4: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4A4 Isl1/2 Hb9 Lhx3 , S4A4 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4B1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B1 Isl1/2 Hb9 Lhx3 , S4B1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4B2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B2 Isl1/2 Hb9 , S4B2 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4B3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B3 Isl1/2 Hb9 , S4B3 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4B4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4B4 Isl1/2 Hb9 Lhx3 , S4B4 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4C1: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C1 Isl1/2 Hb9 Lhx3 , S4C1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4C2: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C2 Isl1/2 Hb9 Lhx3 , S4C2 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4C3: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C3 Isl1/2 Hb9 Lhx3 , S4C3 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4C4: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4C4 Isl1/2 Hb9 , S4C4 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4D1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D1 Isl1/2 Hb9 Lhx3 , S4D1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4D2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D2 Isl1/2 Hb9 , S4D2 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4D3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D3 Isl1/2 Hb9 Lhx3 , S4D3 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4D4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4D4 Isl1/2 Hb9 Lhx3 , S4D4 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4E thoracic Foxp1+/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1+ (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4E1 Isl1/2 Hb9 Lhx3 , S4E1 Isl1/2 Hb9 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4E1 Isl1/2 Hb9 , S4E1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4E thoracic Foxp1-/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1tm1Eem (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4E2 Isl1/2 Hb9 Lhx3 , S4E2 Isl1/2 Hb9 In the absence of Foxp1 the remaining hypaxial motor column and medial motor column neurons were clustered and well segregated.
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4E2 Isl1/2 Hb9 Lhx3 , S4E2 Isl1/2 Hb9 In the absence of Foxp1 the remaining hypaxial motor column and medial motor column neurons were clustered and well segregated.
Specimen S4E brachial Foxp1+/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1+ (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4E3 Isl1/2 Hb9 , S4E3 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4E3 Isl1/2 Hb9 , S4E3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4E brachial Foxp1-/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1tm1Eem (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4E4 Isl1/2 Hb9 , S4E4 Isl1/2 Hb9 Lhx3 In the absence of Foxp1 the remaining hypaxial motor column and medial motor column neurons were clustered and well segregated.
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4E4 Isl1/2 Hb9 , S4E4 Isl1/2 Hb9 Lhx3 In the absence of Foxp1 the remaining hypaxial motor column and medial motor column neurons were clustered and well segregated.
Specimen S4F thoracic HoxA/HoxC+/+: embryonic day 12.5 (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4F1 Isl1/2 Hb9 , S4F1 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4F1 Isl1/2 Hb9 Lhx3 , S4F1 Isl1/2 Hb9 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4F thoracic HoxA/HoxCdelta/delta: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10tm1Mrc, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4F2 Isl1/2 Hb9 Lhx3 , S4F2 Isl1/2 Hb9 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining hypaxial motor column and medial motor column neurons was unaffected.
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S4F2 Isl1/2 Hb9 Lhx3 , S4F2 Isl1/2 Hb9 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining hypaxial motor column and medial motor column neurons was unaffected.
Specimen S4F brachial HoxA/HoxC+/+: embryonic day 12.5 (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4F3 Isl1/2 Hb9 , S4F3 Isl1/2 Hb9 Lhx3 Expression was detected in hypaxial motor column neurons (Mnx1+;Isl1/2+).
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4F3 Isl1/2 Hb9 Lhx3 , S4F3 Isl1/2 Hb9 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4F brachial HoxA/HoxCdelta/delta: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10tm1Mrc, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Triple labeled: green - Mnx1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); blue - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4F4 Isl1/2 Hb9 Lhx3 , S4F4 Isl1/2 Hb9 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining hypaxial motor column and medial motor column neurons was unaffected.
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S4F4 Isl1/2 Hb9 Lhx3 , S4F4 Isl1/2 Hb9 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining hypaxial motor column and medial motor column neurons was unaffected.
Specimen S7B center: embryonic day 12.5 (more )
Note: Double labeled: red - Mnx1; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord cervical region spinal cord motor neuron Present S7B center
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03/18/2025
MGI 6.24 		The Jackson Laboratory