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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8209108
Gene symbol: Mecom
Gene name: MDS1 and EVI1 complex locus
Results
Specimen 7A brachial (Foxp1): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7A Foxp1 Mecom
TS20: spinal cord brachial region lateral motor column neuron Absent 7A Foxp1 Mecom Mecom did not co-localize with Foxp1 in lateral motor column neurons.
Specimen 7A brachial (Lhx3): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7A Lhx3 Mecom Expression was detected in medial motor column neurons and colocalized with Lhx3+ neurons.
Specimen 7A brachial (Hb9): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7A Hb9 Mecom Expression was detected in medial motor column neurons and colocalized with Hb9+ neurons.
Specimen 7A brachial (Isl1/2): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7A Isl1/2 Mecom
Specimen 7B thoracic (Foxp1): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 7B Foxp1 Mecom
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 7B Foxp1 Mecom Expression was excluded from Foxp1+ preganglionic motor column neurons.
Specimen 7B thoracic (Lhx3): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted 7B Lhx3 Mecom Expression was detected in medial motor column neurons. A subset of labeled cells were Lhx3-negative.
Specimen 7B thoracic (Hb9): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 7B Hb9 Mecom Expression was detected in medial motor column neurons and colocalized with Hb9+ neurons.
Specimen 7B thoracic (Isl1/2): embryonic day 12.5 (more )
Note: Double labeled: green - Mecom; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 7B Isl1/2 Mecom
Specimen 7C Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7C Control Lhx3 Mecom
Specimen 7C PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Weak 7C PbxMNdelta Lhx3 Mecom
Specimen 7C HoxCdelta/HoxA+/-: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10+, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Double labeled: green - Mecom; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7C HoxCdelta/HoxA+/- Isl1/2 Mecom Expression was unaffected in the HoxCdelta/HoxA+/- mice.
Specimen not shown E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future spinal cord Present
Specimen S7A e10: embryonic day 10.0 (more )
Note: Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS16: future spinal cord Present Regionally restricted S7A e10 Mecom is expressed in mature Lhx3+ motor neurons, but not detected in Lhx3+ motor neuron precursors or V2 interneurons.
TS16: future spinal cord spinal cord motor neuron Present Regionally restricted S7A e10 Mecom is expressed in mature Lhx3+ motor neurons.
Specimen S7A e10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted S7A e10.5 Mecom is expressed in mature Lhx3+ motor neurons, but not detected in Lhx3+ motor neuron precursors or V2 interneurons.
TS17: future spinal cord spinal cord motor neuron Present Regionally restricted S7A e10.5 Mecom is expressed in mature Lhx3+ motor neurons.
Specimen S7A e11.5: embryonic day 11.5 (more )
Note: Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S7A e11.5 Mecom is expressed in mature Lhx3+ motor neurons, but not detected in Lhx3+ motor neuron precursors or V2 interneurons.
TS19: future spinal cord spinal cord motor neuron Present Regionally restricted S7A e11.5 Mecom is expressed in mature Lhx3+ motor neurons.
Specimen S7B left: embryonic day 12.5 (more )
Note: Rostral cervical level section. Double labeled: green - Mecom; red - Sox5 (assay MGI:8207835).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord cervical region spinal cord medial motor column neuron Present Regionally restricted S7B left Mecom expression did not overlap with the Sox5+ pool.
Specimen S7B center: embryonic day 12.5 (more )
Note: Rostral cervical level section. Double labeled: green - Mecom; red - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord cervical region spinal cord medial motor column neuron Present S7B center
Specimen S7B right: embryonic day 12.5 (more )
Note: Rostral cervical level section. Double labeled: green - Mecom; red - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord cervical region spinal cord medial motor column neuron Present Regionally restricted S7B right Mecom is excluded from non-medial motor column (non-MMC) Lhx3+ populations located at rostral cervical levels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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