Smad5 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8207824
Gene symbol:	Smad5
Gene name:	SMAD family member 5

Antibody:

anti-phospho-Smad

Results

Specimen 2E Control: embryonic day 12.5 (more

)
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Smad5 and Smad1 and Smad9; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord thoracic region	spinal cord motor neuron	Present	Regionally restricted	2E Control	Expression was in preganglionic motor column neurons.

Specimen 2E PbxMNdelta: embryonic day 12.5; Olig2^tm1(cre)Tmj/Olig2⁺, Pbx1^tm1Koss/Pbx1^tm1Koss, Pbx3^tm1Mlc/Pbx3^tm1Mlc (more

)
Note: Conditional mutant. Double labeled: green - Smad5 and Smad1 and Smad9; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord thoracic region	spinal cord motor neuron	Absent		2E PbxMNdelta

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24