|
Allele Symbol Allele Name Allele ID |
Tg(Lck-cre)1Jtak transgene insertion 1, Junji Takeda MGI:3655254 |
| Summary |
22 genotypes |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• despite normal thymus development and a complete rescue of embryonic lethality, mice display a reduction in the number of Runx1+ T cells
|
|
• despite normal thymus development and a complete rescue of embryonic lethality, mice display a reduction in the number of Runx1+ T cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• positive selection of CD4+ cells is severely impaired compared to in DO11.10 TCR control mice
|
|
• positive selection of CD4+ cells is severely impaired compared to in DO11.10 TCR control mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• CD4+ or CD8+ single positive thymocytes
• reduced CD4+CD8int intermediate cells
|
|
• CD4+ or CD8+ single positive thymocytes
• reduced CD4+CD8int intermediate cells
|
|
• CD4+ or CD8+ single positive thymocytes
• reduced CD4+CD8int intermediate cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal Cd4+ single positive thymocytes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• small and fragmented
|
|
• reduced CD4+ single positive thymocytes
• reduced CD4+CD8int intermediate cells
|
|
• small and fragmented
|
|
• reduced CD4+ single positive thymocytes
• reduced CD4+CD8int intermediate cells
|
|
• small and fragmented
|
|
• reduced CD4+ single positive thymocytes
• reduced CD4+CD8int intermediate cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• reduced CD4+CD8int intermediate cells
|
|
• reduced CD4+CD8int intermediate cells
|
|
• reduced CD4+CD8int intermediate cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• slightly in the thymus and lymph nodes
|
|
• maturation of double positive thymocytes to single positive thymocytes is impaired compared to in wild-type mice
|
|
• mice exhibit a marked decrease in CD4+ T cells in the thymus compared with wild-type mice
• peripheral CD4+ T cells in the spleen and lymph node are decreased compared to in wild-type mice
|
|
• mice exhibit a moderate decrease in CD8+ T cells in the thymus compared with wild-type mice
• peripheral CD8+ T cells in the spleen and lymph node are decreased compared to in wild-type mice
|
|
• slightly in the thymus and lymph nodes
|
|
• maturation of double positive thymocytes to single positive thymocytes is impaired compared to in wild-type mice
|
|
• mice exhibit a marked decrease in CD4+ T cells in the thymus compared with wild-type mice
• peripheral CD4+ T cells in the spleen and lymph node are decreased compared to in wild-type mice
|
|
• mice exhibit a moderate decrease in CD8+ T cells in the thymus compared with wild-type mice
• peripheral CD8+ T cells in the spleen and lymph node are decreased compared to in wild-type mice
|
|
• slightly in the thymus and lymph nodes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• positive selection of CD8+ cells in female mice is impaired compared to in H-Y TCR control mice
• male mice fail to exhibit negative selection of double positive and CD8+ T cells unlike H-Y TCR control mice
• Staphylococcal enterotoxin B (SEB)-treated mice fail to exhibit a decrease in TCR Vbeta8+ thymocytes unlike similarly treated H-Y TCR control mice
|
|
• in male mice
|
|
• in male mice
|
|
• in female mice
|
|
• in male mice
|
|
• positive selection of CD8+ cells in female mice is impaired compared to in H-Y TCR control mice
• male mice fail to exhibit negative selection of double positive and CD8+ T cells unlike H-Y TCR control mice
• Staphylococcal enterotoxin B (SEB)-treated mice fail to exhibit a decrease in TCR Vbeta8+ thymocytes unlike similarly treated H-Y TCR control mice
|
|
• in male mice
|
|
• in male mice
|
|
• in female mice
|
|
• in male mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• CD8+ single positive thymocytes
|
|
• CD8+ single positive thymocytes
|
|
• CD8+ single positive thymocytes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• CD4+ single positive thymocytes
|
|
• CD4+ single positive thymocytes
|
|
• CD4+ single positive thymocytes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• impaired T cell chemotaxis to CXCL12 as measured using chemotaxis chambers
|
|
• thymocytes numbers are significantly reduced
|
|
• impaired T cell chemotaxis to CXCL12 as measured using chemotaxis chambers
|
|
• thymocytes numbers are significantly reduced
|
|
• the incidence, but not the severity, of collagen induced arthritis is significantly reduced
|
|
• the incidence, but not the severity, of collagen induced arthritis is significantly reduced
|
|
• impaired T cell chemotaxis to CXCL12 as measured using chemotaxis chambers
|
|
• thymocytes numbers are significantly reduced
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• T cells fail to exhibit an anti-apoptotic effect in response to IL-6 unlike in wild-type mice
|
|
• proliferate in response to IL-6 plus anti-CD3, IL-2 or IL-6 plus ConA stimulation in decreased or absent unlike wild-type mice
• however, proliferation in response to IL-7 is normal
|
|
• T cells fail to exhibit an anti-apoptotic effect in response to IL-6 unlike in wild-type mice
|
|
• proliferate in response to IL-6 plus anti-CD3, IL-2 or IL-6 plus ConA stimulation in decreased or absent unlike wild-type mice
• however, proliferation in response to IL-7 is normal
|
|
• T cells fail to exhibit an anti-apoptotic effect in response to IL-6 unlike in wild-type mice
|
|
• proliferate in response to IL-6 plus anti-CD3, IL-2 or IL-6 plus ConA stimulation in decreased or absent unlike wild-type mice
• however, proliferation in response to IL-7 is normal
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• in older mice numbers of activated/memory T cells increases; however, these cells contain only unrecombined alleles
|
|
• markedly reduced numbers of peripheral T cells
• at 4 - 6 months of age almost no naive T cells are found in the spleen and lymph nodes
|
|
• severe decrease in the number of CD24low mature cells while the number of CD24high immature cells is similar to controls
|
|
• severe decrease in the number of CD24low mature cells while the number of CD24high immature cells is similar to controls
|
|
• no detectable regulatory T cells in the thymus or spleen at 6 weeks of age
|
|
• decreased numbers of single positive T cells, especially CD8+ cells, while the numbers of double negative and double positive T cells is similar to controls
|
|
• seen at 4 - 6 months of age
|
|
• increase in the numbers of myeloid lineage cells (macrophages, neutrophils) but almost a complete absence of naive T cells is detected at 4 - 6 months of age
|
|
• dose-dependent induction of cell death in response to TNF stimulation unlike controls cells which are viable after TNF stimulation
|
|
• impaired proliferation in response to anti-CD3 stimulation with or without co-stimulation by anti-CD28
|
|
• seen at 4 - 6 months of age
|
|
• at 4 - 6 months of age, loss of goblet cells is seen in the large intestine but not in the small intestine
|
|
• prominent ulceration is seen at 4 - 6 months of age in the large, but not the small, intestine
|
|
• stiff colon present at 4 - 6 months of age
|
|
• seen at 4 - 6 months of age
|
|
• severe diarrhea beginning at 4 - 6 months of age
|
|
• begins at 4 - 6 months of age
|
|
• seen at 4 - 6 months of age
|
|
• increase in the numbers of myeloid lineage cells (macrophages, neutrophils) but almost a complete absence of naive T cells is detected at 4 - 6 months of age
|
|
• in older mice numbers of activated/memory T cells increases; however, these cells contain only unrecombined alleles
|
|
• markedly reduced numbers of peripheral T cells
• at 4 - 6 months of age almost no naive T cells are found in the spleen and lymph nodes
|
|
• severe decrease in the number of CD24low mature cells while the number of CD24high immature cells is similar to controls
|
|
• severe decrease in the number of CD24low mature cells while the number of CD24high immature cells is similar to controls
|
|
• no detectable regulatory T cells in the thymus or spleen at 6 weeks of age
|
|
• decreased numbers of single positive T cells, especially CD8+ cells, while the numbers of double negative and double positive T cells is similar to controls
|
|
• seen at 4 - 6 months of age
|
|
• increase in the numbers of myeloid lineage cells (macrophages, neutrophils) but almost a complete absence of naive T cells is detected at 4 - 6 months of age
|
|
• dose-dependent induction of cell death in response to TNF stimulation unlike controls cells which are viable after TNF stimulation
|
|
• impaired proliferation in response to anti-CD3 stimulation with or without co-stimulation by anti-CD28
|
|
• at 4 - 6 months of age, loss of goblet cells is seen in the large intestine but not in the small intestine
|
|
• impaired proliferation in response to anti-CD3 stimulation with or without co-stimulation by anti-CD28
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increase in intestinal epithelial permeability indicating defective barrier function
|
|
• mice develop spontaneous colitis
• mice in which gut commensal bacteria is removed by treatment with four antibiotics do not exhibit colitis
• -6-8 week old mutants transferred with TCRalphabeta+CD8alpha+ IELS show significant amelioration of colitis, however transfer with CD8+ T or natural killer cells has no effect on colitis
|
|
• drastic reduction in the frequency and absolute number of TCR beta+ T cells in the mesenteric lymph nodes
• however, the colonic lamina propria contains TCR beta+ T cells
• some mice have up to 10 times more T cells in colonic lamina propria than wild-type mice
|
|
• the majority of TCR alpha beta+ T cells in the mesenteric lymph nodes and colonic lamina propria are CD4+ T cells compared to wild-type mice
• accumulation of CD4+ T cells in the colonic lamina propria
|
|
• the TCR beta+NK1.1+ natural killer T cell population in the thymus and liver and alpha-GalCer/CD1d-reacted natural killer T cell population in the liver are hardly detectable in mice
|
|
• reduction in the frequencies and absolute numbers of TCR beta+ intestinal intraepithelial lymphocytes (IEL) in both the small and large intestine
• both the CD8 alpha-alpha+ and CD8 alpha-beta+ cell populations of TCR beta+ IELs are almost completely absent
• the CD4+CD8 alpha-alpha+ cell population in TCR beta+CD8 beta- cells is decreased in the small and large intestine
• the colon exclusive TCR beta+CD4-CD8 alpha-CD8 beta- cell population is absent
• the remaining TCR beta+ IEL fraction is CD4+ T cells
• thymic IEL precursors (TCR alpha-beta+CD4-CD8-NK1.1-) are decreased in both frequency and absolute number; this reduction is due to almost complete loss of CD103+ cell population coexpressing both CD5 and CD122
|
|
• increase in the frequency of TCR gamma-delta+ IELs in the small and large intestines and the absolute numbers of these IELs are increased in the colon but not the small intestine
|
|
• frequency of regulatory T cells is higher in mesenteric lymph nodes and lower in colonic lamina propria in mice older than 10 weeks of age, however, absolute numbers of regulatory T cells are unchanged
|
|
• reduction in frequency of CD25+Foxp3+ cells in CD4 single positive thymocytes of 10 week old mice that do not have colitis
|
|
• thymic IEL precursors (TCRalphabeta+CD4-CD8-NK1.1-) stimulated with an agonistic anti-CD3 antibody in vitro do not show enlargement of cell size and induced expression of CD25, IL-2Ralpha chain as seen in wild-type cells
• thymic IEL precursors treated with the agonistic anti-CD3 antibody show inefficient induction of CD8alpha induction
|
|
• CD4+ T cells from colonic lamina propria and mesenteric lymph nodes stimulated with PMA plus ionomycin exhibit a more robust production of IFN-gamma and IL-17A than in wild-type cells
|
|
• in an in vitro suppression assay, regulatory T cells show less suppressive activity than wild-type T cells, indicating failure of suppressive function of the leaky regulatory T cells
|
|
• colons show increased mRNA levels of proinflammatory cytokines and RegIII-beta/gamma proteins (antimicrobial peptides)
• elevation of expression of IFN-gamma and IL-17
|
|
• mice develop spontaneous colitis
• mice in which gut commensal bacteria is removed by treatment with four antibiotics do not exhibit colitis
• -6-8 week old mutants transferred with TCRalphabeta+CD8alpha+ IELS show significant amelioration of colitis, however transfer with CD8+ T or natural killer cells has no effect on colitis
|
|
• drastic reduction in the frequency and absolute number of TCR beta+ T cells in the mesenteric lymph nodes
• however, the colonic lamina propria contains TCR beta+ T cells
• some mice have up to 10 times more T cells in colonic lamina propria than wild-type mice
|
|
• the majority of TCR alpha beta+ T cells in the mesenteric lymph nodes and colonic lamina propria are CD4+ T cells compared to wild-type mice
• accumulation of CD4+ T cells in the colonic lamina propria
|
|
• the TCR beta+NK1.1+ natural killer T cell population in the thymus and liver and alpha-GalCer/CD1d-reacted natural killer T cell population in the liver are hardly detectable in mice
|
|
• reduction in the frequencies and absolute numbers of TCR beta+ intestinal intraepithelial lymphocytes (IEL) in both the small and large intestine
• both the CD8 alpha-alpha+ and CD8 alpha-beta+ cell populations of TCR beta+ IELs are almost completely absent
• the CD4+CD8 alpha-alpha+ cell population in TCR beta+CD8 beta- cells is decreased in the small and large intestine
• the colon exclusive TCR beta+CD4-CD8 alpha-CD8 beta- cell population is absent
• the remaining TCR beta+ IEL fraction is CD4+ T cells
• thymic IEL precursors (TCR alpha-beta+CD4-CD8-NK1.1-) are decreased in both frequency and absolute number; this reduction is due to almost complete loss of CD103+ cell population coexpressing both CD5 and CD122
|
|
• increase in the frequency of TCR gamma-delta+ IELs in the small and large intestines and the absolute numbers of these IELs are increased in the colon but not the small intestine
|
|
• frequency of regulatory T cells is higher in mesenteric lymph nodes and lower in colonic lamina propria in mice older than 10 weeks of age, however, absolute numbers of regulatory T cells are unchanged
|
|
• reduction in frequency of CD25+Foxp3+ cells in CD4 single positive thymocytes of 10 week old mice that do not have colitis
|
|
• thymic IEL precursors (TCRalphabeta+CD4-CD8-NK1.1-) stimulated with an agonistic anti-CD3 antibody in vitro do not show enlargement of cell size and induced expression of CD25, IL-2Ralpha chain as seen in wild-type cells
• thymic IEL precursors treated with the agonistic anti-CD3 antibody show inefficient induction of CD8alpha induction
|
|
• CD4+ T cells from colonic lamina propria and mesenteric lymph nodes stimulated with PMA plus ionomycin exhibit a more robust production of IFN-gamma and IL-17A than in wild-type cells
|
|
• in an in vitro suppression assay, regulatory T cells show less suppressive activity than wild-type T cells, indicating failure of suppressive function of the leaky regulatory T cells
|
|
• colons show increased mRNA levels of proinflammatory cytokines and RegIII-beta/gamma proteins (antimicrobial peptides)
• elevation of expression of IFN-gamma and IL-17
|
|
• CD4+ T cells from colonic lamina propria and mesenteric lymph nodes stimulated with PMA plus ionomycin exhibit a more robust production of IFN-gamma than in wild-type cells
|
|
• CD4+ T cells from colonic lamina propria and mesenteric lymph nodes stimulated with PMA plus ionomycin exhibit a more robust production of IL-17A than in wild-type cells
|
|
• large numbers of bacteria are detected in the livers
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mice lack follicular dendritic cells and no immune complex binding is observed
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• exhibit higher IFN-gamma secretion by activated splenocytes
• both CD4+ and CD8+ T cells produce higher levels or IFN-gamma upon activation
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• IL-17 producing CD4+ T cells differentiated in vitro and induced with IL-6 and TGF-beta with the anti-TCR antibody results in stronger induction of IFN-gamma producing cells and a marked reduction of IL-17 producing cells in contrast to similarly treated wild-type cells
• CD4+ T cells preferentially differentiate into Th1 cells and not Th17 unlike wild-type cells
|
|
• CD4+ T cells are resistant to the suppressive effects of TGF-beta on IGN-gamma production unlike wild-type cells
|
|
• mice are less susceptible to experimental autoimmune encephalomyelitis (EAE) and produce less IL-17 following EAE compared to wild-type mice
|
|
• IL-17 producing CD4+ T cells differentiated in vitro and induced with IL-6 and TGF-beta with the anti-TCR antibody results in stronger induction of IFN-gamma producing cells and a marked reduction of IL-17 producing cells in contrast to similarly treated wild-type cells
• CD4+ T cells preferentially differentiate into Th1 cells and not Th17 unlike wild-type cells
|
|
• CD4+ T cells are resistant to the suppressive effects of TGF-beta on IGN-gamma production unlike wild-type cells
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased percentage of immature single positive cells in irradiated mice
|
|
• in irradiated mice
|
|
• increased percentage in irradiated mice
|
|
• decreased percent in irradiated mice
|
|
• decreased percent in irradiated mice
|
|
• decreased percent in irradiated mice
|
|
• thymic lymphomas in 4 of 5 irradiated mice
|
|
• attenuated radiation-induced cell cycle arrest at the S phase in immature single positive cells of irradiated mice
|
|
• in irradiated mice
|
|
• increased percentage of immature single positive cells in irradiated mice
|
|
• in irradiated mice
|
|
• increased percentage in irradiated mice
|
|
• decreased percent in irradiated mice
|
|
• decreased percent in irradiated mice
|
|
• decreased percent in irradiated mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• T cells exhibit decreased proliferation in response to anti-CD3 stimulation
|
|
• the number of thymoyctes is reduced by 40-60% compared to controls
|
|
• the percentage of double-negative thymocytes is about double that of control
|
|
• the percentage of double-positive thymocytes is reduced by 40-60%
|
|
• the number of CD4+ T cells in the spleen is reduced by about 50% compared to controls
|
|
• the number of CD8+ T cells in the spleen is reduced by about 60% compared to controls
|
|
• the percentage of single-positive thymocytes is reduced by 40-60%
|
|
• T cells secrete less IFN-gamma in response to anti-CD3 stimulation
|
|
• T cells secrete less IL-2 in response to anti-CD3 stimulation
|
|
• T cells exhibit decreased proliferation in response to anti-CD3 stimulation
|
|
• the number of thymoyctes is reduced by 40-60% compared to controls
|
|
• the percentage of double-negative thymocytes is about double that of control
|
|
• the percentage of double-positive thymocytes is reduced by 40-60%
|
|
• the number of CD4+ T cells in the spleen is reduced by about 50% compared to controls
|
|
• the number of CD8+ T cells in the spleen is reduced by about 60% compared to controls
|
|
• the percentage of single-positive thymocytes is reduced by 40-60%
|
|
• the number of thymoyctes is reduced by 40-60% compared to controls
|
|
• T cells exhibit decreased proliferation in response to anti-CD3 stimulation
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• BrdU incorporation of double-negative (DN) T cells is 15.1% versus 26.6% in controls, indicating that cell proliferation accompanied with DN-to-DP transition after beta-selection is suppressed
• Cdkn1a (p21Cip1) mRNA expression is increased in DN3 (Lin-CD25+CD117-) populations
• however, expression of Myc (c-Myc) mRNA and protein in DN3 is normal
|
|
• early T-cell precursor (ETP) numbers are significantly reduced
• total thymocyte number is reduced by ~25-fold
|
|
• abnormal differentiation from DN3a to DN3b and DN4 compartments
• however, DNA rearrangement of the TCRbeta locus is normal
|
|
• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced
• however, % of icTCRb+ cells in DN3 is normal
|
|
• DN3 thymocyte number is reduced by >2.5-fold
• % of DN3b cells (defined by CD27hi and increased cell size) in DN3 population is decreased while upregulation of CD27 expression is reduced, suggesting defects in pre-TCR signaling
• however, % of intracellular TCRbeta (icTCRb)+ cells in DN3 is normal
|
|
• DN4 thymocyte number is reduced by 20-fold
• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced
|
|
• severe block in the transition from the double-negative DN3/4 stage to the double-positive (DP) stage
• most cells accumulate at the DN3 (CD25+CD117-) stage where beta-selection occurs
• however, Notch signaling is not impaired at the DN3 stage and gamma-delta T cell development is normal
|
|
• significant decrease in CD4 single-positive (SP) population
|
|
• significant decrease in CD8 immature single-positive (SP) population
|
|
• BrdU incorporation of double-negative (DN) T cells is 15.1% versus 26.6% in controls, indicating that cell proliferation accompanied with DN-to-DP transition after beta-selection is suppressed
• Cdkn1a (p21Cip1) mRNA expression is increased in DN3 (Lin-CD25+CD117-) populations
• however, expression of Myc (c-Myc) mRNA and protein in DN3 is normal
|
|
• early T-cell precursor (ETP) numbers are significantly reduced
• total thymocyte number is reduced by ~25-fold
|
|
• abnormal differentiation from DN3a to DN3b and DN4 compartments
• however, DNA rearrangement of the TCRbeta locus is normal
|
|
• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced
• however, % of icTCRb+ cells in DN3 is normal
|
|
• DN3 thymocyte number is reduced by >2.5-fold
• % of DN3b cells (defined by CD27hi and increased cell size) in DN3 population is decreased while upregulation of CD27 expression is reduced, suggesting defects in pre-TCR signaling
• however, % of intracellular TCRbeta (icTCRb)+ cells in DN3 is normal
|
|
• DN4 thymocyte number is reduced by 20-fold
• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced
|
|
• severe block in the transition from the double-negative DN3/4 stage to the double-positive (DP) stage
• most cells accumulate at the DN3 (CD25+CD117-) stage where beta-selection occurs
• however, Notch signaling is not impaired at the DN3 stage and gamma-delta T cell development is normal
|
|
• significant decrease in CD4 single-positive (SP) population
|
|
• significant decrease in CD8 immature single-positive (SP) population
|
|
• early T-cell precursor (ETP) numbers are significantly reduced
• total thymocyte number is reduced by ~25-fold
|
|
• DN3 thymocyte number is reduced by >2.5-fold
• % of DN3b cells (defined by CD27hi and increased cell size) in DN3 population is decreased while upregulation of CD27 expression is reduced, suggesting defects in pre-TCR signaling
• however, % of intracellular TCRbeta (icTCRb)+ cells in DN3 is normal
|
|
• DN4 thymocyte number is reduced by 20-fold
• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced
|
|
• BrdU incorporation of double-negative (DN) T cells is 15.1% versus 26.6% in controls, indicating that cell proliferation accompanied with DN-to-DP transition after beta-selection is suppressed
• Cdkn1a (p21Cip1) mRNA expression is increased in DN3 (Lin-CD25+CD117-) populations
• however, expression of Myc (c-Myc) mRNA and protein in DN3 is normal
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• more than half of mice
|
| N |
• surviving mice exhibit no auto-immune disease symptoms up to 4 months of age
• mice exhibit normal numbers of regulatory T cells in the thymus and spleen
|
|
• DSS-treated mice exhibit accelerated body weight loss, severe intestinal inflammation, increased Th1 cells and attenuated increase of regulatory T cells in the colon and cecum lamina propria compared with control mice
|
|
• increased under Th1-, Th17- and induced regulatory T cell-skewing conditions
|
|
• reduced under Th17-skewing conditions
|
|
• CD44highCD62Llow-activated and IFN-gamma CD4+ T cells
|
|
• in DSS-treated mice
|
|
• reduced regulatory T cell differentiation under induced regulatory T cell-skewing conditions
|
|
• in DSS-treated mice
|
|
• DSS-treated mice exhibit accelerated body weight loss, severe intestinal inflammation, increased Th1 cells and attenuated increase of regulatory T cells in the colon and cecum lamina propria compared with control mice
|
|
• accelerated in DSS-treated mice
|
|
• increased under Th1-, Th17- and induced regulatory T cell-skewing conditions
|
|
• reduced under Th17-skewing conditions
|
|
• CD44highCD62Llow-activated and IFN-gamma CD4+ T cells
|
|
• in DSS-treated mice
|
|
• reduced regulatory T cell differentiation under induced regulatory T cell-skewing conditions
|
|
• in DSS-treated mice
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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