Tg(DO11.10)10Dlo
Transgene Detail

Summary

• Symbol: Tg(DO11.10)10Dlo
• Name: transgene insertion 10, Dennis Y Loh
• MGI ID: MGI:2664398
• Synonyms: CO11.10 TCR-alphabeta, D10, DO.10 Tg, DO11, DO.11, DO-11.10, DO11.10, Do11.10 alphabetaTCR, Do11.10 alphabeta TCR, DO11.10 alphabeta-TCR, DO.11.10 TCR, DO11.10 TCR, DO11.10TCRalphabeta, DO11.10 TCR Tg, DO11 TCR, DO-Tg, OVA-TCR^tg, Tg(DO11.10)Tcra/Tcrb, TgN(DO11.10)10Dlo, TgN(DO11.10)10Loh, Tg(TcraDO11.10,TcrbDO11.10)10Dlo
• Transgene: Tg(DO11.10)10Dlo Location: unknown Genetic Position: Chr18, Syntenic
• Alliance: Tg(DO11.10)10Dlo page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1 x BALB/c

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(DO11.10)10Dlo expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: This double transgene contains rearranged Tcra and Tcrb genes and is expressed in the majority of T cells. These rearranged transgenes from the DO11.10 T cell hybridoma encode a chicken ovalbumin-specific MHC class II (I-A^d)-restricted T cell receptor. The allele was created by injection of a mix of the two separate transgenes Tg(TcraDO11.10)#Dlo and Tg(TcrbDO11.10)#Dlo into oocytes. (J:25536, J:61321)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available

Notes

Mice carrying this pair of MHC class II restricted rearranged Tcra and Tcrb T cell receptor transgenes on a H2^d background react to ovalbumin (OVA) peptide antigen. Intraperitoneal administration of OVA to transgenic mice results in a rapid deletion of the immature CD4⁺ CD8⁺ TCR^lo thymocytes with progression to mature thymocytes. Apoptosis of cortical thymocytes in these mice occurs within 20 hours of treatment.

References

• Original: J:70572 Murphy KM, et al., Induction by antigen of intrathymic apoptosis of CD4+CD8+TCRlo thymocytes in vivo. Science. 1990 Dec 21;250(4988):1720-3
• All: 758 reference(s)