Map3k3^tm1Kuro
Targeted Allele Detail

Summary

• Symbol: Map3k3^tm1Kuro
• Name: mitogen-activated protein kinase kinase kinase 3; targeted mutation 1, Tomohiro Kurosaki
• MGI ID: MGI:3840862
• Synonyms: MEKK3^flox
• Gene: Map3k3 Location: Chr11:105975439-106046272 bp, + strand Genetic Position: Chr11, 68.89 cM
• Alliance: Map3k3^tm1Kuro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147103
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A single loxP site was introduced into intron 10 and a floxed neomycin cassette was introduced into intron 11. Cre recombinase was transiently expressed in correctly targeted ES cells to remove the neomycin cassette, leaving exon 11 floxed. Exon 11 encodes the ATP-binding site (K391) essential for the kinase activity of the protein product. (J:147103)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map3k3 Mutation: 24 strains or lines available

References

• Original: J:147103 Shinohara H, et al., Regulation of NF-kappaB-dependent T cell activation and development by MEKK3. Int Immunol. 2009 Apr;21(4):393-401
• All: 1 reference(s)