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Tg(TcraTcrb)1100Mjb
Transgene Detail
Summary
Symbol: Tg(TcraTcrb)1100Mjb
Name: transgene insertion 1100, Michael J Bevan
MGI ID: MGI:3054907
Synonyms: OT-1, OT1, OT1 TCR, OT-I, OTI, OT-I(OVA)TCR, OT-I TCR, OT-ITCR, OVA-tcr-I, OVA-Tg, TCR-OT-I, Tg(OT-I), Tg(TcrOva)1100Mjb, Tg(TcrOVA)1100 mjb
Transgene: Tg(TcraTcrb)1100Mjb  Location: unknown  Genetic Position: Chr3, Syntenic
Alliance: Tg(TcraTcrb)1100Mjb page
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(TcraTcrb)1100Mjb expresses 2 genes
 
Mutation detailsTransgenic mice carry two coinjected constructs containing rearranged mouse TCR alpha (Tcra-V2, Tcra-J(alpha26)) and TCR beta (Tcrb-V5, Tcrb-D(beta2), Tcrb-J(beta2.6)) chains isolated from T cell clone 149.42. This transgenic T cell receptor recognizes ovalbumin residues 257-264 (SIINFEKL) in the context of H2Kb. The rearranged Tcra (alpha chain) cDNA specific for ovalbumin was isolated from T cells (clone AD10) and is expressed under the control of a 2-kb fragment of the H-2Kb promoter, a 700-bp fragment including the Ig H chain enhancer located 5' to the promoter, and a 1.7-kb BamHI-PstI fragment containing the 3' half of the human adult 8-globin gene (including part of exon 2, the exon 2-exon 3 C.F6 TCR a-chain gene that was truncated at the 3' XbaI site intron, exon 3) and polyadenylation signal. The Tcrb (beta chain) construct consists of a genomic fragment containing the Tcrb coding and endogenous promoter regions from a T cell clone (B3) specific for ovalbumin. (J:92867)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Tumor Data
List all tumor models in MMHCdb carrying Tg(TcraTcrb)1100Mjb
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Notes
Phenotypic Similarity to Human Syndrome: Anaplastic Large Cell Lymphoma J: 235890 in double Tg(Cd4-NPM/ALK)N1Ingh and Tg(TcraTcrb)1100Mjb mice.
References
Original:  J:92867 Hogquist KA, et al., T cell receptor antagonist peptides induce positive selection. Cell. 1994 Jan 14;76(1):17-27
All:  1738 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/01/2024
MGI 6.24
The Jackson Laboratory