Analysis Tools|
Allele Symbol Allele Name Allele ID |
Tg(Pou3f4-cre)32Cren transgene insertion 32, Bryan Crenshaw III MGI:2158470 |
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Summary |
13 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased cell death without a decrease in cell proliferation
|
|
• increased cell death without a decrease in cell proliferation
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• proliferation of cells declined by E10
• 45% at E11 to 50% at E11.5
• E11 proliferation rate down 35%
• 4.8X increase in progenitor cell death and apoptosis
• higher proportion of differentiated neurons to proliferative cells
• number of neurons unchanged
|
|
• changes similar to those seen in the spinal cord
• tissue mass of the midbrain was reduced
• progenitor domains in the midbrain and other areas of the brain reduced
• increased apoptosis
|
|
• ventricular zone absent at E12
• reduced area occupied by progenitor cells
• ventral progenitor are absent by E11.5
• absent dorsally by E12
• Larger area occupied by differentiated neurons(as determined immunohistochemically and gene markers)
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• at P7, numbers of Zfp276-positive oligodendrocytes in the spinal cord only reach about 7% of those in controls, as identified by Olig2 co-staining
|
|
• at P7, numbers of Zfp276-positive oligodendrocytes in the spinal cord only reach about 7% of those in controls, as identified by Olig2 co-staining
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• despite the absence of visceral or branchial motoneurons, the main trunk of the facial nerve is present
|
|
• visceromotor neurons are not produced
• absence of visceral or branchial motoneurons
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• survived up to 17 days
|
|
• shafts usually fail to form in follicles
|
|
• wavy
|
|
• misshapen and expanded dermal papillae
|
|
• misangled
• not as deep into the dermis
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• ectopic distal phalanges are sometimes found
|
|
• although reduced digits is typical, polydactyly sometimes occurs
|
|
• polysyndactyly is common
|
|
• if hindlimbs are present then they are highly malformed
• loss of ventral structures of hindlimbs
|
|
• in many but not all animals
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• whole brain is smaller than wild-type brain at E18.5
|
|
|
• normal distribution of interneurons in cerebral cortex is lost in mutants, with Calb1+ interneurons restricted to subcortical and subventricular regions
|
|
|
• olfactory bulbs are nearly absent at E18.5
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased proliferation of cells by E10
• 110% at E11 to 300% at E11.5
• E11 proliferation rate increased 1.4X
• 6.7% increase in progenitor cell death and apoptosis
• lower proportion of differentiated neurons to proliferative cells
|
|
• changes similar to those seen in the spinal cord
• tissue mass of the midbrain was increased
• increased proliferation in all areas of the brain
• progenitor domains of the midbrain and other areas of the brain increased
• increased apoptosis
|
|
• enlarged ventricular zone at E10.5
• increased area occupied by neural progenitor cells
• smaller area occupied by differentiated neurons (as determined immunohistochemically and by activity of genes expressed in progenitor cells)
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• expression of late differentiation markers in dorsal spinal neurons is reduced indicating a defect in terminal differentiation
|
|
• almost complete loss of small diameter nociceptive fibers in the dorsal horn
• reduction in the innervation of TrkA+ and aquaporin 1+ sensory neurons in the dorsal horn
|
|
• in dorsal spinal neurons neurites are severely reduced and remaining structures often appear misshapen
• cultured neurons from the superficial dorsal horn show a decrease in the overall number, length and branching frequency of neurites
|
|
• almost complete loss of small diameter nociceptive fibers in the dorsal horn at E16.5
|
|
• the superficial zone is invariably compressed and the nuclei appear compacted
• expression of late differentiation markers in dorsal spinal neurons is reduced indicating a defect in terminal differentiation
• in dorsal spinal neurons neurites are severely reduced and remaining structures often appear misshapen
• the density of DiI positive fibers is greatly reduced and the remaining fibers appear disorganized at E16.5
• almost complete loss of small diameter nociceptive fibers in the dorsal horn
|
|
• the success rate for evoked currents is reduced in dorsal horn neurons
|
|
• expression of late differentiation markers in dorsal spinal neurons is reduced indicating a defect in terminal differentiation
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mice die at late embryonic stages
|
| N |
• mice exhibit normal basolateral and radial fibers, and glia- and motor neuron generation
|
|
• increased apoptosis in the spinal cord at E11.5 and E12.5
|
|
• at E13.5, cultured neurospheres exhibit reduced self-renewal compared with wild-type cells
|
|
• at E10.5 and E11.5, spinal cord cells exhibit early cell-cycle exit compared with wild-type cells
|
|
• at E11.5, spinal cords lack a well-organized ventricular zone and exhibit dysplasia, likely due to neuroepithelial cells invading the lumen of the neural tube, compared with wild-type mice
• rossette-like structures are present in the ventricular zone of the spinal cord unlike in wild-type mice
• at E14.5, the ventricular zone and neural tube lumen are missing unlike in wild-type mice
|
|
• at E11.5, spinal cords lack a well-organized ventricular zone and exhibit dysplasia, likely due to neuroepithelial cells invading the lumen of the neural tube, compared with wild-type mice
• rossette-like structures are present in the ventricular zone of the spinal cord unlike in wild-type mice
• at E14.5, the ventricular zone and neural tube lumen are missing unlike in wild-type mice
|
|
• increased apoptosis in the spinal cord at E11.5 and E12.5
|
|
• at E13.5, cultured neurospheres exhibit reduced self-renewal compared with wild-type cells
|
|
• at E10.5 and E11.5, spinal cord cells exhibit early cell-cycle exit compared with wild-type cells
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 09/30/2025 MGI 6.24 |
|
|
|
||
