About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:8270491
Gene symbol: Zfp503
Gene name: zinc finger protein 503
Reporter: lacZ
Antibody: anti-B-galactosidase (4600-1409)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
Results
Specimen 1q: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: hypothalamus Present 1q' , 1q
Specimen 1r: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: hypothalamus Present 1r' , 1r
Specimen 1s: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: hypothalamus Present 1s
Specimen 1t: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: hypothalamus Present 1t
Specimen 1u: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: corpus striatum Present 1u
Specimen 1v: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: corpus striatum Present 1v
Specimen 1w: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: corpus striatum Present 1w
Specimen 1x: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: corpus striatum Present 1x
TS26: globus pallidus Absent 1x
Specimen 2a: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Zfp503 reporter; green - Ppp1r1b (assay MGI:8270621).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2a' , 2a Expression is detected in all striatal projection neurons, which overlaps with Ppp1r1b-staining.
Specimen 2b: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Ppp1r1b (assay MGI:8270621).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2b , 2b' In Nolz1<bgal//bgal> mutant embryos striatal projection neurons were labelled with beta galactosidase, but lacked Ppp1r1b.
Specimen 2g: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Rostral striatum. Double labeled: red - Zfp503 reporter; green - Bcl11b (assay MGI:8270623).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2g' , 2g Expression is detected in all striatal projection neurons, which overlaps with Bcl11b-staining.
Specimen 2h: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Caudal striatum. Double labeled: red - Zfp503 reporter; green - Bcl11b (assay MGI:8270623).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2h' , 2h Expression is detected in all striatal projection neurons, which overlaps with Bcl11b-staining.
TS26: globus pallidus Absent 2h' , 2h
Specimen 2i: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Bcl11b (assay MGI:8270623).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2i , 2i' In Nolz1<bgal//bgal> mutant embryos striatal projection neurons were labelled by Bcl11b and beta galactosidase.
Specimen 2j: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Bcl11b (assay MGI:8270623).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2j' , 2j In Nolz1<bgal//bgal> mutant embryos striatal projection neurons were labelled by Bcl11b and beta galactosidase.
TS26: globus pallidus Absent 2j' , 2j
Specimen 4a Nolz1bgal/+: embryonic day 11.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: green - Zfp503 reporter; red - Six3 (assay MGI:8270658).
Structure Cell Type Level Pattern Image Note
TS20: corpus striatum striatum neuron Present Regionally restricted 4a WT SIX3 BGAL Beta galactosidase is detected in striatal projection neurons and is co-labeled with Six3.
Specimen 4a Nolz1bgal/bgal: embryonic day 11.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - Zfp503 reporter; red - Six3 (assay MGI:8270658).
Structure Cell Type Level Pattern Image Note
TS20: corpus striatum striatum neuron Present Regionally restricted 4a Nolz1-/- SIX3 BGAL Selective upregulation of Six3 was observed in cells lacking Zfp503 (Nolz1) expression indicated by beta galactosidase in Nolz1<bgal/bgal> mutants.
Specimen S1c WT: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Zfp503 reporter; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: midbrain Present S1c WT NOLZ1/bGAL TH
Specimen S1c Nolz1bgal/bgal: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: midbrain Present S1c Nolz1bgal/bgal NOLZ1/bGAL TH The axon guidance phenotype observed in embryos lacking Zfp503 (Nolz1) expression was not caused by the mislocalization or elimination of mutant cells as beta galactosidase expression in Nolz1b<Gal/bGal> mutant embryos was similar to heterozygous embryos in the midbrain.
Specimen S2b Nolz1bgal/+: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Zfp503 reporter; green - Nkx2-1 (assay MGI:8272321).
Structure Level Pattern Image Note
TS26: globus pallidus Absent S2b Nolz1bgal/+ bottom
Specimen S2b Nolz1bgal/bgal: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Zfp503 reporter; green - Nkx2-1 (assay MGI:8272321).
Structure Level Pattern Image Note
TS26: globus pallidus Absent S2b Nolz1bgal/bgal bottom
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
10/06/2026
MGI 6.29 		The Jackson Laboratory