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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8270658
Gene symbol: Six3
Gene name: sine oculis-related homeobox 3
Antibody: Anti-Six3 (200-201-A26)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present). Two Six3 antibodies were listed MGI:8270357 and MGI:8270359; the one used in this assay was not specified.
Results
Specimen 4a Nolz1bgal/+: embryonic day 11.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Six3; green - Zfp503 reporter (assay MGI:8270491).
Structure Cell Type Level Pattern Image Note
TS20: corpus striatum striatum neuron Present Regionally restricted 4a WT SIX3 BGAL Expression is detected in striatopallidal projection neurons.
Specimen 4a Nolz1bgal/bgal: embryonic day 11.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Six3; green - Zfp503 reporter (assay MGI:8270491).
Structure Cell Type Level Pattern Image Note
TS20: corpus striatum striatum neuron Strong Regionally restricted 4a Nolz1-/- SIX3 BGAL Selective upregulation of SIX3 was observed in cells lacking NOLZ1 expression indicated by beta galactosidase in Nolz1<bgal/bgal> mutants.
Specimen S4f WT E10.5 injected: embryonic day 15.5 (more )
Note: Double labeled: red - Six3; green - BrdU injected at E10.5.
Structure Cell Type Level Pattern Image Note
TS23: corpus striatum striatum neuron Present Regionally restricted S4f WT E10.5 injected boxed area , S4f WT E10.5 injected Expression is detected in striatopallidal projection neurons.
Specimen S4f Nolz1-/- E10.5 injected: embryonic day 15.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Six3; green - BrdU injected at E10.5.
Structure Cell Type Level Pattern Image Note
TS23: corpus striatum striatum neuron Present Regionally restricted S4f Nolz1-/- E10.5 injected boxed area , S4f Nolz1-/- E10.5 injected Expression is detected in striatopallidal projection neurons.
Specimen S4f WT E11.5 injected: embryonic day 15.5 (more )
Note: Double labeled: red - Six3; green - BrdU injected at E11.5.
Structure Cell Type Level Pattern Image Note
TS23: corpus striatum striatum neuron Present Regionally restricted S4f WT E11.5 injected boxed area , S4f WT E11.5 injected Expression is detected in striatopallidal projection neurons.
Specimen S4f Nolz1-/- E11.5 injected: embryonic day 15.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Six3; green - BrdU injected at E11.5.
Structure Cell Type Level Pattern Image Note
TS23: corpus striatum striatum neuron Strong Regionally restricted S4f Nolz1-/- E11.5 injected boxed area , S4f Nolz1-/- E11.5 injected Expression is expanded in striatopallidal projection neurons in the mutant.
Specimen S4f WT E12.5 injected: embryonic day 15.5 (more )
Note: Double labeled: red - Six3; green - BrdU injected at E12.5.
Structure Cell Type Level Pattern Image Note
TS23: corpus striatum striatum neuron Present Regionally restricted S4f WT E12.5 injected , S4f WT E12.5 injected boxed area Expression is detected in striatopallidal projection neurons.
Specimen S4f Nolz1-/- E12.5 injected: embryonic day 15.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Six3; green - BrdU injected at E12.5.
Structure Cell Type Level Pattern Image Note
TS23: corpus striatum striatum neuron Strong Regionally restricted S4f Nolz1-/- E12.5 injected , S4f Nolz1-/- E12.5 injected boxed area Expression is expanded in striatopallidal projection neurons in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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