Gene Expression Data
Assay Details
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Assay
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Reference:
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J:290119
Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8270621
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Gene symbol:
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Ppp1r1b
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Gene name:
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protein phosphatase 1, regulatory inhibitor subunit 1B
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Antibody:
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Anti-DARPP-32 (H-62: sc-11365)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
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Results
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Specimen
2a:
embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503 +
(more  )
Note: Double labeled: green - Ppp1r1b; red - Zfp503 reporter (assay MGI:8270491).
Specimen
2a:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Zfp503tm1.1Bpb/Zfp503+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ppp1r1b; red - Zfp503 reporter (assay MGI:8270491).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: corpus striatum |
striatum neuron |
Present |
Regionally restricted |
2a'
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2a
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Expression is detected in striatal projection neurons and overlaps with beta-galactosidase reporter staining.
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Specimen
2b:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Zfp503tm1.1Bpb/Zfp503tm1.1Bpb
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ppp1r1b; red - Zfp503 reporter (assay MGI:8270491).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: corpus striatum |
Present |
Clusters |
2b'
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2b
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In Nolz1<bgal//bgal> mutant embryos striatal projection neurons were labelled beta galactosidase, but lacked Ppp1r1b. Expression appears in clusters in
mutant striatum that are abnormally fasciculated axonal tracts.
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Analysis Tools