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Gene Expression Data
Assay Details
Assay
Reference: J:241009 Himmelstein DS, et al., SHH E176/E177-Zn2+ conformation is required for signaling at endogenous sites. Dev Biol. 2017 Apr 15;424(2):221-235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6761790
Gene symbol: Tubg2
Gene name: tubulin, gamma 2
Antibody: anti-gamma-tubulin
Detection system: Secondary antibody coupled to Cy3
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 1D: embryonic day 9.5 (more )
Note: Dorsal region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1D Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 1E: embryonic day 9.5 (more )
Note: Intermediate region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1E Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 1F: embryonic day 9.5 (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1F Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 1H: embryonic day 9.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761570).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1H Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 1I: embryonic day 9.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: notochord Present Punctate 1I Expressed in cilia basal bodies.
Specimen 1J: embryonic day 9.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761570).
Structure Level Pattern Image Note
TS15: notochord Present Punctate 1J Expressed in cilia basal bodies.
Specimen 1K: embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761570).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1K Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 1L: embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1L Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2A/A': embryonic day 8.75 (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761574).
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2A' , 2A Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2D/D': embryonic day 8.75; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761574).
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2D' , 2D Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2B/B': embryonic day 8.75 (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2B' , 2B Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2E/E': embryonic day 8.75; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2E , 2E' Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2G/G': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761574).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2G , 2G' Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2I/I': embryonic day 9.5; Shhtm1Chg/Shhtm2Chg, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant. Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761574).
Structure Level Pattern Image Note
TS15: future spinal cord Present 2I' , 2I
Specimen 2K/K': embryonic day 9.5; Ift172wim/Ift172wim (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761574).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2K' , 2K Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2H/H': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2H' , 2H Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2J/J': embryonic day 9.5; Shhtm1Chg/Shhtm2Chg, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant. Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2J' , 2J Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2L/L': embryonic day 9.5; Ift172wim/Ift172wim (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2L' , 2L Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 2M: embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761574).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2M Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 6A/A': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761570).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6A' , 6A Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 6B/B': embryonic day 9.5; Shhtm1.1Jhdk/Shhtm1Chg (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761570).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6B , 6B' Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 6C/C': embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761570).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6C , 6C' Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 6D/D': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6D , 6D' Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 6E/E': embryonic day 9.5; Shhtm1.1Jhdk/Shhtm1Chg (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6E' , 6E Expressed in cilia basal bodies in puncta along the apical spinal cord.
Specimen 6F/F': embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Ventral region. Double labeled: red - Tubg1 and Tubg2; green - Shh (assay MGI:6761562).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6F' , 6F Expressed in cilia basal bodies in puncta along the apical spinal cord.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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