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Gene Expression Data
Assay Details
Assay
Reference: J:241009 Himmelstein DS, et al., SHH E176/E177-Zn2+ conformation is required for signaling at endogenous sites. Dev Biol. 2017 Apr 15;424(2):221-235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6761570
Gene symbol: Shh
Gene name: sonic hedgehog
Antibody: anti-Shh (H-160; sc9024)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1H: embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Present Diffuse 1H The antibody detected non-cilia basal bodies associated expression.
Specimen 1J: embryonic day 9.5 (more )
Note: Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: notochord Present Diffuse 1J The antibody detected non-cilia basal bodies associated expression.
Specimen 1K: embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 1K
Specimen 6A/A': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Present Diffuse 6A' , 6A
Specimen 6B/B': embryonic day 9.5; Shhtm1.1Jhdk/Shhtm1Chg (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Absent 6B , 6B'
Specimen 6C/C': embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Absent 6C' , 6C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory