Shh^tm1.1Jhdk
Targeted Allele Detail

Summary

• Symbol: Shh^tm1.1Jhdk
• Name: sonic hedgehog; targeted mutation 1.1, Jhumku Kohtz
• MGI ID: MGI:5906825
• Synonyms: Shh^E177A
• Gene: Shh Location: Chr5:28661838-28672099 bp, - strand Genetic Position: Chr5, 14.39 cM
• Alliance: Shh^tm1.1Jhdk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241009
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site flanked stop and neomycin resistance gene cassette was inserted into the 5' UTR and a mutation was engineered in exon 2. The mutation changes glutamic acid codon 177 (GAA) to an alanine codon (GCG)(p.Glu177Ala). The neo and stop cassette was removed through subsequent cre-mediated recombination. PCR and cDNA sequencing confirm the expression of the mutated transcript from this allele. (J:241009)

Expression

In Mice Carrying this Mutation:

23 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shh Mutation: 45 strains or lines available

References

• Original: J:241009 Himmelstein DS, et al., SHH E176/E177-Zn2+ conformation is required for signaling at endogenous sites. Dev Biol. 2017 Apr 15;424(2):221-235
• All: 1 reference(s)