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Gene Expression Data
Assay Details
Assay
Reference: J:241009 Himmelstein DS, et al., SHH E176/E177-Zn2+ conformation is required for signaling at endogenous sites. Dev Biol. 2017 Apr 15;424(2):221-235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6761574
Gene symbol: Shh
Gene name: sonic hedgehog
Antibody: anti-SHHCL;M/P
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A/A': embryonic day 8.75 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS14: neural tube floor plate Present Diffuse 2A , 2A'
TS14: notochord Present Diffuse 2A
Specimen 2D/D': embryonic day 8.75; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS14: neural tube floor plate Present Diffuse 2D , 2D' Reduced expression compared with the wild-type.
TS14: notochord Present Diffuse 2D Reduced expression compared with the wild-type.
Specimen 2G/G': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Present Diffuse 2G' , 2G
TS15: notochord Present Diffuse 2G
Specimen 2I/I': embryonic day 9.5; Shhtm1Chg/Shhtm2Chg, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant. Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Trace Diffuse 2I , 2I' Reduced or absent expression compared with the wild-type.
TS15: notochord Present Diffuse 2I
Specimen 2K/K': embryonic day 9.5; Ift172wim/Ift172wim (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Absent 2K , 2K'
TS15: notochord Weak 2K
Specimen 2M: embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 2M
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory