About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:241009 Himmelstein DS, et al., SHH E176/E177-Zn2+ conformation is required for signaling at endogenous sites. Dev Biol. 2017 Apr 15;424(2):221-235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6761574
Gene symbol: Shh
Gene name: sonic hedgehog
Antibody: anti-SHHCL;M/P
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A/A': embryonic day 8.75 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS14: neural tube floor plate Present Diffuse 2A , 2A'
TS14: notochord Present Diffuse 2A
Specimen 2D/D': embryonic day 8.75; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS14: neural tube floor plate Present Diffuse 2D , 2D' Reduced expression compared with the wild-type.
TS14: notochord Present Diffuse 2D Reduced expression compared with the wild-type.
Specimen 2G/G': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Present Diffuse 2G' , 2G
TS15: notochord Present Diffuse 2G
Specimen 2I/I': embryonic day 9.5; Shhtm1Chg/Shhtm2Chg, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant. Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Trace Diffuse 2I , 2I' Reduced or absent expression compared with the wild-type.
TS15: notochord Present Diffuse 2I
Specimen 2K/K': embryonic day 9.5; Ift172wim/Ift172wim (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: neural tube floor plate Absent 2K , 2K'
TS15: notochord Weak 2K
Specimen 2M: embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572) and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 2M
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory