Shh^tm2Chg
Targeted Allele Detail

Summary

• Symbol: Shh^tm2Chg
• Name: sonic hedgehog; targeted mutation 2, Chin Chiang
• MGI ID: MGI:3628824
• Synonyms: Shh^flox, Shh^floxC
• Gene: Shh Location: Chr5:28661838-28672099 bp, - strand Genetic Position: Chr5, 14.39 cM
• Alliance: Shh^tm2Chg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109101
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to flank the sonic hedgehog processing domain in exon 3 with loxP sites. The second loxP site was followed by a Myc epitope tag with a stop codon and an FRT site flanked neomycin resistance gene cassette downstream of the stop codon. The neo cassette was removed by crossing with mice expressing flp recombinase. (J:109101)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	18 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shh Mutation: 45 strains or lines available

References

• Original: J:109101 Li Y, et al., Cholesterol modification restricts the spread of Shh gradient in the limb bud. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6548-53
• All: 5 reference(s)