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Gene Expression Data
Assay Details
Assay
Reference: J:241009 Himmelstein DS, et al., SHH E176/E177-Zn2+ conformation is required for signaling at endogenous sites. Dev Biol. 2017 Apr 15;424(2):221-235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6761562
Gene symbol: Shh
Gene name: sonic hedgehog
Antibody: anti-SHHCL/P
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1D: embryonic day 9.5 (more )
Note: Dorsal region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1D Expressed in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 1E: embryonic day 9.5 (more )
Note: Intermediate region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1E Expression was greater in the intermediate region, compared to dorsal. Expressed in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 1F: embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1F Expressed in puncta associated with the cilia basal bodies in embryonic spinal cord ventricles. Expression was greater in the ventral region, compared to dorsal.
Specimen 1G: embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - alpha-tubulin.
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 1G Expression was greater in the ventral region, compared to dorsal. Expressed in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 1I: embryonic day 9.5 (more )
Note: Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: notochord Present Punctate 1I Expressed in puncta associated with the cilia basal bodies.
Specimen 1L: embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 1L
Specimen 2B/B': embryonic day 8.75 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2B' , 2B Expressed in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 2E/E': embryonic day 8.75; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2E , 2E' Increased expression in both diffuse and large aggregates lining the ventricle and at a distance from the ventricle.
Specimen 2C/C': embryonic day 8.75 (more )
Note: Ventral region. Double labeled: green - Shh; red - alpha-tubulin.
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2C' , 2C Expressed in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 2F/F': embryonic day 8.75; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: Ventral region. Double labeled: green - Shh; red - alpha-tubulin.
Structure Level Pattern Image Note
TS14: future spinal cord Present Regionally restricted 2F' , 2F Increased expression in both diffuse and large aggregates lining the ventricle and at a distance from the ventricle.
Specimen 2H/H': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2H , 2H' Expressed in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 2J/J': embryonic day 9.5; Shhtm1Chg/Shhtm2Chg, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant. Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 2J , 2J' Reduced expression in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 2L/L': embryonic day 9.5; Ift172wim/Ift172wim (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 2L , 2L'
Specimen 6D/D': embryonic day 9.5 (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6D' , 6D Expressed in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle.
Specimen 6E/E': embryonic day 9.5; Shhtm1.1Jhdk/Shhtm1Chg (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 6E' , 6E Increased expression in puncta restricted to the apical domain of neuroepithelial cells lining the ventricle, compared with the wild-type.
Specimen 6F/F': embryonic day 9.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Ventral region. Double labeled: green - Shh; red - Tubg1 (assay MGI:6761572 and Tubg2 (assay MGI:6761790).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 6F , 6F'
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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