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Gene Expression Data
Assay Details
Assay
Reference: J:264612 Morgani SM, et al., A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Dev Biol. 2018 Sep 1;441(1):104-126
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6286386
Gene symbol: Gata6
Gene name: GATA binding protein 6
Antibody: anti-Gata6 (AF1700)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were described for this gene product; the one used in this assay was not detailed. Either MGI:5912154 or MGI:6286278 could have been used.
Results
Specimen 3A 16c: embryonic day 3.0; Spry4tm1Hadj/Spry4+ (more )
Note: 16 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS3: compacted morula Present 3A 16c Merge + Nuclei , 3A 16c Gata6
Specimen 3A 31c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 31 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS4: compacted morula Present 3A 31c Gata6 , 3A 31c Merge + Nuclei
Specimen 3A 59c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 59 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3A 59c Merge + Nuclei , 3A 59c Gata6
Specimen 3A 89c: embryonic day 3.75; Spry4tm1Hadj/Spry4+ (more )
Note: 89 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3A 89c Gata6 , 3A 89c Merge + Nuclei
Specimen 3A 106c: embryonic day 4.0; Spry4tm1Hadj/Spry4+ (more )
Note: 106 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A 106c Merge + Nuclei , 3A 106c Gata6
Specimen 3A 148c: embryonic day 4.5; Spry4tm1Hadj/Spry4+ (more )
Note: 148 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A 148c Gata6 , 3A 148c Merge + Nuclei
Specimen 3A 187c: embryonic day 5.0; Spry4tm1Hadj/Spry4+ (more )
Note: 187 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A 187c Gata6 , 3A 187c Merge + Nuclei
Specimen 3B 15c: embryonic day 3.0; Spry4tm1Hadj/Spry4+ (more )
Note: 15 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS3: compacted morula Present 3B 15c Merge + Nuclei , 3B 15c Gata6
Specimen 3B 30c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 30 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: compacted morula Present 3B 30c Merge + Nuclei , 3B 30c Gata6
Specimen 3B 57c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 57 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3B 57c Gata6 , 3B 57c Merge + Nuclei
Specimen 3B 86c: embryonic day 3.75; Spry4tm1Hadj/Spry4+ (more )
Note: 86 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3B 86c Merge + Nuclei , 3B 86c Gata6
Specimen 3B 91c: embryonic day 4.0; Spry4tm1Hadj/Spry4+ (more )
Note: 91 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B 91c Merge + Nuclei , 3B 91c Gata6
Specimen 3B 141c: embryonic day 4.5; Spry4tm1Hadj/Spry4+ (more )
Note: 141 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B 141c Gata6 , 3B 141c Merge + Nuclei
Specimen 3B 159c: embryonic day 5.0; Spry4tm1Hadj/Spry4+ (more )
Note: 159 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B 159c Merge + Nuclei , 3B 159c Gata6
Specimen 4A 58c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 58 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A 58c Merge + Nuclei , 4A 58c Gata6
Specimen 4A 62c: embryonic day 3.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt, Spry4tm1Hadj/Spry4+ (more )
Note: 62 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Trace 4A 62c Fgf4-/- Gata6 , 4A 62c Fgf4-/- Merge + Nuclei
Specimen 4A 62c Fgfr1-/-: embryonic day 3.5; Fgfr1tm1Jrt/Fgfr1tm1Jrt, Spry4tm1Hadj/Spry4+ (more )
Note: 62 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Trace Single cells 4A 62c Fgfr1-/- Gata6 , 4A 62c Fgfr1-/- Merge + Nuclei
Specimen 4A 38c: embryonic day 3.5; Fgfr2tm1Dor/Fgfr2tm1Dor, Spry4tm1Hadj/Spry4+, Tg(CAG-cre)13Miya/0 (more )
Note: Conditional mutant. 38 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A 38c Fgfr2-/- Gata6 , 4A 38c Fgfr2-/- Merge + Nuclei
Specimen 4B 74c: embryonic day 3.75; Spry4tm1Hadj/Spry4+ (more )
Note: 74 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B 74c Merge + Nuclei , 4B 74c Gata6
Specimen 4B 68c: embryonic day 3.75; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt, Spry4tm1Hadj/Spry4+ (more )
Note: 68 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Trace 4B 68c Merge + Nuclei , 4B 68c Gata6
Specimen 4B 83c: embryonic day 3.75; Fgfr1tm1Jrt/Fgfr1tm1Jrt, Spry4tm1Hadj/Spry4+ (more )
Note: 83 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Ambiguous 4B 83c Merge + Nuclei , 4B 83c Gata6
Specimen 4B 77c: embryonic day 3.75; Fgfr2tm1Dor/Fgfr2tm1Dor, Spry4tm1Hadj/Spry4+, Tg(CAG-cre)13Miya/0 (more )
Note: Conditional mutant. 77 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B 77c Merge + Nuclei , 4B 77c Gata6
Specimen 4C 115c: embryonic day 4.0; Spry4tm1Hadj/Spry4+ (more )
Note: 115 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 4C 115c Merge + Nuclei , 4C 115c Gata6
Specimen 4C 111c: embryonic day 4.0; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt, Spry4tm1Hadj/Spry4+ (more )
Note: 111 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Trace 4C 111c Merge + Nuclei , 4C 111c Gata6
Specimen 4C 98c: embryonic day 4.0; Fgfr1tm1Jrt/Fgfr1tm1Jrt, Spry4tm1Hadj/Spry4+ (more )
Note: 98 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Ambiguous 4C 98c Gata6 , 4C 98c Merge + Nuclei
Specimen 4C 104c: embryonic day 4.0; Fgfr2tm1Dor/Fgfr2tm1Dor, Spry4tm1Hadj/Spry4+, Tg(CAG-cre)13Miya/0 (more )
Note: Conditional mutant. 104 cells. Triple labeled: blue - Gata6; red - Nanog (assay MGI:6286387); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 4C 104c Merge + Nuclei , 4C 104c Gata6
Specimen 6A MIP (3D): embryonic day 5.5; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - Sox2 (assay MGI:6286394); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 6A MIP (3D) Merge , 6A MIP (3D) Gata6
Specimen 6A Sagittal: embryonic day 5.5; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - Sox2 (assay MGI:6286394); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 6A Sagittal Merge , 6A Sagittal Gata6
Specimen 6A Trans: embryonic day 5.5; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - Sox2 (assay MGI:6286394); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 6A Trans Merge , 6A Trans Gata6
Specimen 6B MIP (3D): embryonic day 6.5-6.75; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - T (assay MGI:6286395); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: endoderm Present 6B MIP (3D) Gata6 , 6B MIP (3D) Merge
TS9: mesoderm Present 6B MIP (3D) Gata6 , 6B MIP (3D) Merge
Specimen 6B Sagittal: embryonic day 6.5-6.75; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - T (assay MGI:6286395); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: endoderm Present 6B Sagittal Gata6 , 6B Sagittal Merge
TS9: mesoderm Present 6B Sagittal Gata6 , 6B Sagittal Merge
Specimen 6B Trans: embryonic day 6.5-6.75; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - T (assay MGI:6286395); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: endoderm Present 6B Trans Gata6 , 6B Trans Merge
TS9: mesoderm Present 6B Trans Gata6 , 6B Trans Merge
Specimen 7A MIP (3D): embryonic day 7.75-8.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - T (assay MGI:6286395); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS11: mesoderm Present Non-Uniform 7A MIP (3D) Merge , 7A MIP (3D) Gata6 Expression level was intermediate in mesoderm closest to the primitive streak and high in cells further from the primitive streak.
Specimen 7A Sagittal: embryonic day 7.75-8.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - T (assay MGI:6286395); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS11: mesoderm Present Non-Uniform 7A Sagittal Merge , 7A Sagittal Gata6 , 7A Anterior (1) Merge , 7A Anterior (1) Gata6 , 7A Posterior (2) Gata6 , 7A Posterior (2) Merge Expression level was intermediate in mesoderm closest to the primitive streak and high in cells further from the primitive streak.
Specimen 7B: embryonic day 7.75-8.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - T (assay MGI:6286395); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS11: mesoderm Present Non-Uniform 7B Gata6 , 7B Merge Expression level was intermediate in mesoderm closest to the primitive streak and high in cells further from the primitive streak.
Specimen S6B MIP (3D): embryonic day 6.75-7.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - Foxa2 (assay MGI:6286498); green - Spry4 reporter (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: endoderm Present S6B MIP (3D) Merge , S6B MIP (3D) Gata6
TS9: mesoderm Present S6B MIP (3D) Merge , S6B MIP (3D) Gata6
Specimen S6B Sagittal: embryonic day 6.75-7.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - Foxa2 (assay MGI:6286498); green - Spry4 reporter (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: endoderm Present S6B Sagittal Merge , S6B Sagittal Gata6
TS9: mesoderm Present S6B Sagittal Merge , S6B Sagittal Gata6
Specimen S6B Transverse: embryonic day 6.75-7.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: blue - Gata6; red - Foxa2 (assay MGI:6286498); green - Spry4 reporter (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: endoderm Present S6B Transverse Gata6 , S6B Transverse Merge
TS9: mesoderm Present S6B Transverse Gata6 , S6B Transverse Merge
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05/07/2024
MGI 6.23 		The Jackson Laboratory