Gene Expression Image Detail

Reference

Figure

ID

J:264612 Morgani SM, et al., Dev Biol. 2018 Sep 1;441(1):104-126

6

MGI:6285967

Image

Caption

A-B. Confocal images of representative E5.5 (A, scale bars, 25 um) and E6.5-E6.75 (B, scale bars, 50 um) Spry4^H2B-Venus/+ embryos after wholemount immunofluorescence staining. Upper to lower panels show: maximum intensity projections (MIP), optical sagittal sections and optical transverse sections. Nuclei are stained with Hoechst. Dashed white line indicates plane of transverse optical section. Bracket demarcates the primitive streak. C. High magnification confocal image of the primitive streak region of an E6.5-E6.75 Spry4^H2B-Venus/+ embryo. Epi, epiblast; VE, visceral endoderm; ExE, extraembryonic ectoderm; ExM, extraembryonic mesoderm; PS, primitive streak; Meso, mesoderm; Pr, proximal, Ds, distal; A, anterior; P, posterior; R, right; L, left. D. Transverse confocal optical section of an E6.5-E6.75 embryo marked with the regions defined for quantification in E. Regions were defined based on positional information and include epiblast (Epi, gray) - inner epithelial cells, endoderm (En, pink) - outer epithelial cell layer, primitive streak and mesoderm (PS/Meso, orange) - cells leaving the epithelial layer at the posterior of the Epi and migrating between the Epi and outer endoderm layers.

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2018.06.017 Dev Biol 441: 104-126, Morgani SM; Saiz N; Garg V; Raina D; Simon CS; Kang M; Arias AM; Nichols J; Schroter C; Hadjantonakis AK, A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Copyright 2018

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A MIP (3D) Merge	MGI:6286386 (Gata6) MGI:6286394 (Sox2) MGI:6286316 (Spry4)
A MIP (3D) Venus	MGI:6286316 (Spry4)
A MIP (3D) Sox2	MGI:6286394 (Sox2)
A MIP (3D) Gata6	MGI:6286386 (Gata6)
A Sagittal Merge	MGI:6286386 (Gata6) MGI:6286394 (Sox2) MGI:6286316 (Spry4)
A Sagittal Venus	MGI:6286316 (Spry4)
A Sagittal Sox2	MGI:6286394 (Sox2)
A Sagittal Gata6	MGI:6286386 (Gata6)
A Trans Merge	MGI:6286386 (Gata6) MGI:6286394 (Sox2) MGI:6286316 (Spry4)
A Trans Venus	MGI:6286316 (Spry4)
A Trans Sox2	MGI:6286394 (Sox2)
A Trans Gata6	MGI:6286386 (Gata6)
B MIP (3D) Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
B MIP (3D) Venus	MGI:6286316 (Spry4)
B MIP (3D) Brachyury	MGI:6286395 (T)
B MIP (3D) Gata6	MGI:6286386 (Gata6)
B Sagittal Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
B Sagittal Venus	MGI:6286316 (Spry4)
B Sagittal Brachury	MGI:6286395 (T)
B Sagittal Gata6	MGI:6286386 (Gata6)
B Trans Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
B Trans Venus	MGI:6286316 (Spry4)
B Trans Brachyury	MGI:6286395 (T)
B Trans Gata6	MGI:6286386 (Gata6)
C Merge	MGI:6286316 (Spry4) MGI:6286395 (T)
C Venus	MGI:6286316 (Spry4)
C Brachyury	MGI:6286395 (T)
D	MGI:6286316 (Spry4)