Gene Expression Image Detail

Reference

Figure

ID

J:264612 Morgani SM, et al., Dev Biol. 2018 Sep 1;441(1):104-126

7

MGI:6285969

Image

Caption

A. Confocal images of a representative E7.75-E8.0 Spry4^H2B-Venus/+ embryo after wholemount immunofluorescence. Upper to lower panels show: maximum intensity projections (MIP), optical sagittal sections and high magnification images of anterior and posterior regions. Nuclei are stained with Hoechst. Dashed white line (B) indicates plane of transverse optical section shown in panel B. White boxes, 1 and 2, indicate regions shown in lower panel high magnification anterior and posterior images. B. Image showing a confocal transverse optical section through the E7.75-E8.0 Spry4^H2B-Venus/+ embryo in panel A. Epi, epiblast; VE, visceral endoderm; PS, primitive streak; M, mesoderm; A, anterior; P, posterior; R, right; L, left; Pr, proximal; Ds, distal. Bracket demarcates the primitive streak. Scale bars, 100 um. C. Left-most panel shows the transverse confocal optical section of embryo from panel B marked with the regions defined for quantification in D and E. Regions include epiblast (Epi, gray), endoderm (En, pink) - identified as the outermost cell layer, primitive streak (PS, red) - identified as cells leaving the epithelial layer at the posterior of the epiblast, mesoderm A (Ma, orange) defined as GATA6-low cells closest to the PS, mesoderm B (Mb, blue) defined as GATA6-high cells further from the PS, mesoderm C (Mc, green), cells that expressed neither GATA6 nor BRACHYURY that had migrated most anteriorly. Other panels display a look up table (LUT) visualization of fluorescence intensity of each fluorophore in panel B using a range indicator to show high levels of expression in red and low levels in blue.

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2018.06.017 Dev Biol 441: 104-126, Morgani SM; Saiz N; Garg V; Raina D; Simon CS; Kang M; Arias AM; Nichols J; Schroter C; Hadjantonakis AK, A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Copyright 2018

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A MIP (3D) Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
A MIP (3D) Venus	MGI:6286316 (Spry4)
A MIP (3D) Brachyury	MGI:6286395 (T)
A MIP (3D) Gata6	MGI:6286386 (Gata6)
A Sagittal Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
A Sagittal Venus	MGI:6286316 (Spry4)
A Sagittal Brachyury	MGI:6286395 (T)
A Sagittal Gata6	MGI:6286386 (Gata6)
A Anterior (1) Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
A Anterior (1) Venus	MGI:6286316 (Spry4)
A Anterior (1) Brachyury	MGI:6286395 (T)
A Anterior (1) Gata6	MGI:6286386 (Gata6)
A Posterior (2) Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
A Posterior (2) Venus	MGI:6286316 (Spry4)
A Posterior (2) Brachyury	MGI:6286395 (T)
A Posterior (2) Gata6	MGI:6286386 (Gata6)
B Merge	MGI:6286386 (Gata6) MGI:6286316 (Spry4) MGI:6286395 (T)
B Venus	MGI:6286316 (Spry4)
B Brachyury	MGI:6286395 (T)
B Gata6	MGI:6286386 (Gata6)