About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:264612 Morgani SM, et al., A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Dev Biol. 2018 Sep 1;441(1):104-126
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6286395
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachyury (AF2085)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 6B MIP (3D): embryonic day 6.5-6.75; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: red - T; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 6B MIP (3D) Brachyury , 6B MIP (3D) Merge
TS9: mesoderm Present 6B MIP (3D) Brachyury , 6B MIP (3D) Merge
TS9: primitive streak Present 6B MIP (3D) Brachyury , 6B MIP (3D) Merge
Specimen 6B Sagittal: embryonic day 6.5-6.75; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: red - T; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 6B Sagittal Merge , 6B Sagittal Brachury
TS9: mesoderm Present 6B Sagittal Merge , 6B Sagittal Brachury
TS9: primitive streak Present 6B Sagittal Merge , 6B Sagittal Brachury
Specimen 6B Trans: embryonic day 6.5-6.75; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: red - T; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 6B Trans Merge , 6B Trans Brachyury
TS9: mesoderm Present 6B Trans Merge , 6B Trans Brachyury
TS9: primitive streak Present 6B Trans Merge , 6B Trans Brachyury
Specimen 6C: embryonic day 6.5-6.75; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: red - T; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 6C Merge , 6C Brachyury
TS9: primitive streak Present 6C Merge , 6C Brachyury
Specimen 7A MIP (3D): embryonic day 7.75-8.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: red - T; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS11: primitive streak Present 7A MIP (3D) Brachyury , 7A MIP (3D) Merge
TS11: mesoderm Present Non-Uniform 7A MIP (3D) Brachyury , 7A MIP (3D) Merge Expression was highest in mesoderm closest to the primitive streak and weakest in cells further from the primitive streak.
Specimen 7A Sagittal: embryonic day 7.75-8.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: red - T; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS11: primitive streak Present 7A Anterior (1) Merge , 7A Posterior (2) Brachyury , 7A Sagittal Brachyury , 7A Sagittal Merge , 7A Anterior (1) Brachyury , 7A Posterior (2) Merge
TS11: mesoderm Present Non-Uniform 7A Anterior (1) Merge , 7A Posterior (2) Brachyury , 7A Sagittal Merge , 7A Sagittal Brachyury , 7A Posterior (2) Merge , 7A Anterior (1) Brachyury Expression was highest in mesoderm closest to the primitive streak and weakest in cells further from the primitive streak.
Specimen 7B: embryonic day 7.75-8.0; Spry4tm1Hadj/Spry4+ (more )
Note: Triple labeled: red - T; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS11: primitive streak Present 7B Merge , 7B Brachyury
TS11: mesoderm Present Non-Uniform 7B Brachyury , 7B Merge Expression was highest in mesoderm closest to the primitive streak and weakest in cells further from the primitive streak.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory