Rag2 MGI Mouse Gene Detail - MGI:97849 - recombination activating gene 2

Symbol

Rag2
Name

recombination activating gene 2
Synonyms

Rag-2

Feature Type

protein coding gene
IDs

MGI:97849
NCBI Gene: 19374
Alliance

genome feature page
Transcription Start Sites

5 TSS
Regulated by

Rr695557 (1 regulatory region)

Sequence Map

Chr2:101455063-101462874 bp, + strand
From Ensembl annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 2, 53.87 cM
Mapping Data

9 experiments

SNPs within 2kb

284 from dbSNP Build 142
Strain Annotations

19

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_97849	protein coding gene	Chr2:101455057-101462874 (+)
129S1/SvImJ	ENSMUSG00200002313	protein coding gene	Chr2:98257427-98265241 (+)
A/J	ENSMUSG00195000637	protein coding gene	Chr2:98432516-98440340 (+)
AKR/J	ENSMUSG00220001597	protein coding gene	Chr2:98186117-98193925 (+)
BALB/cJ	ENSMUSG00180019079	protein coding gene	Chr2:98241528-98249337 (+)
C3H/HeJ	ENSMUSG00175009830	protein coding gene	Chr2:98510956-98518766 (+)
C57BL/6NJ	ENSMUSG00215015772	protein coding gene	Chr2:98336271-98344082 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0024001	protein coding gene	Chr2:94928459-94936087 (+)
CAST/EiJ	ENSTCUG00005001561	protein coding gene	Chr2:97597186-97604836 (+)
CBA/J	ENSMUSG00210001835	protein coding gene	Chr2:98519583-98527391 (+)
DBA/2J	ENSMUSG00185005578	protein coding gene	Chr2:98238551-98246361 (+)
FVB/NJ	ENSMUSG00205010696	protein coding gene	Chr2:97537082-97544891 (+)
JF1/MsJ	ENSUMUG00000026579	protein coding gene	Chr2:97929467-97937262 (+)
LP/J	ENSMUSG00230000722	protein coding gene	Chr2:99988019-99995828 (+)
NOD/ShiLtJ	ENSMUSG00190021923	protein coding gene	Chr2:98339891-98347700 (+)
NZO/HlLtJ	ENSMUSG00225008470	protein coding gene	Chr2:107878951-107886760 (+)
PWK/PhJ	ENSLUMG00010023416	protein coding gene	Chr2:97916797-97924592 (+)
SPRET/EiJ	ENSMSPG00010008658	protein coding gene	Chr2:99929737-99937357 (+)
WSB/EiJ	ENSIUOG00005001209	protein coding gene	Chr2:98022836-98030647 (+)

Human Ortholog

RAG2, recombination activating 2

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

RAG2, recombination activating 2
Synonyms

RAG-2
Links

HGNC:9832

NCBI Gene ID: 5897

UniProt: P55895
Chr Location

11p12; chr11:36575574-36598279 (-) GRCh38

MGI Vertebrate Homology

Rag2 stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: RAG2
Gene Tree

Rag2

Diseases

2 with Rag2 mouse models; 4 with human RAG2 associations

Human Disease

Mouse Models

Omenn syndrome

IDs

View 1 model

severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, Nk cell-positive

IDs

View 1 model

combined cellular and humoral immune defects with granulomas

IDs

severe combined immunodeficiency

IDs

Click on a disease name to see all genes associated with that disease.

Mutations/Alleles

4 with disease annotations

References

5 with disease annotations

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Phenotype Summary

82 phenotypes from 11 alleles in 16 genetic backgrounds
140 phenotypes from multigenic genotypes
1650 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

45
Chemically induced (ENU)

1
Chemically induced (other)

1
Endonuclease-mediated

22
Radiation induced

1
Targeted

20
Genomic Mutations

4 involving Rag2
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

118 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Homozygotes for targeted null mutations exhibit arrested development of T and B cell maturation at the CD4-8- thymocyte or B220+/CD43+pro-B cell stage due to inability to undergo V(D)J recombination.

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All GO Annotations

38
GO References

25

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

654
Tissues

33
cDNA Data

10
Literature Summary

15

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

ZFIN rag2

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All Sequences

42
RefSeq

4
UniProt

4
CCDS

CCDS16462.1

Ensembl

ENSMUSG00000032864 (Evidence)
NCBI Gene

19374

			Length	Strain/Species	Flank
genomic	ENSMUSG00000032864	Ensembl Gene Model \| MGI Sequence Detail	7812	C57BL/6J	± kb
transcript	ENSMUST00000044031	Ensembl \| MGI Sequence Detail	3424	Not Applicable
polypeptide	ENSMUSP00000038204	Ensembl \| MGI Sequence Detail	527	Not Applicable

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UniProt

4 Sequences
Protein Ontology

PR:000003460 V(D)J recombination-activating protein 2

(term hierarchy)
PDB

2JWO, 2V83, 2V85, 2V86, 2V87, 2V88, 2V89, 4WWX, 5ZDZ, 5ZE0, 5ZE1, 5ZE2, 6CG0, 6CIJ, 6CIK, 6CIL, 6CIM, 6OEM, 6OEN, 6OEO, 6OEP, 6OEQ, 6OER, 6OES, 6OET, 6V0V, 6XNX, 6XNY, 6XNZ, 9JPU, 9JPX, 9JQN, 9JTS, 9JTU
InterPro Domains

IPR011043 Galactose oxidase/kelch, beta-propeller

IPR015915 Kelch-type beta-propeller

IPR025162 Recombination activating protein 2, PHD domain

IPR004321 V-D-J recombination activating protein 2

IPR011011 Zinc finger, FYVE/PHD-type

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All nucleic 25

Genomic 7

cDNA 10

Primer pair 6

Other 2

Microarray probesets 4

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MGD-MRK-13754, MGD-MRK-13756

Summaries

All 2179

Developmental Gene Expression 15

Diseases 5

Gene Ontology 25

Phenotypes 1650
Earliest

J:109968 Roderick TH, et al., Nineteen paracentric chromosomal inversions in mice. Genetics. 1974 Jan;76(1):109-17
Latest

J:390589 Albertini G, et al., The Alzheimer's therapeutic Lecanemab attenuates Abeta pathology by inducing an amyloid-clearing program in microglia. Nat Neurosci. 2026 Jan;29(1):100-110

Rag2 expression regulated by:

Feature	Feature Type	Location	Reference
Rr695557, regulatory region 695557	enhancer	Chr2:101382624-101383235	J:364828

TSS for Rag2:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr19074	Chr2:101455022-101455040 (+)	-32 bp
Tssr19075	Chr2:101455041-101455075 (+)	-5 bp
Tssr19076	Chr2:101455079-101455101 (+)	27 bp
Tssr19077	Chr2:101455114-101455125 (+)	57 bp
Tssr19078	Chr2:101457751-101457766 (+)	2,696 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/01/2026 MGI 6.24