Ctnnb1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279716 Braitsch CM, et al., LATS1/2 suppress NFkappaB and aberrant EMT initiation to permit pancreatic progenitor differentiation. PLoS Biol. 2019 Jul;17(7):e3000382
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8351284
Gene symbol:	Ctnnb1
Gene name:	catenin beta 1

Antibody:	anti-beta-catenin (C-18): sc-1496
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

2F ©

Specimen 2F: embryonic day 14.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: magenta - Ctnnb1; green - Acta2 (assay MGI:8351283).

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		2F

2J ©

Specimen 2J: embryonic day 14.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: magenta - Ctnnb1; green - Acta2 (assay MGI:8351283).

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		2J

3F ©

Specimen 3F: embryonic day 11.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: red - Ctnnb1; green - Vnn1 (assay MGI:8351300).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Ambiguous		3F

3G ©

Specimen 3G: embryonic day 11.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: red - Ctnnb1; green - Vnn1 (assay MGI:8351300).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Ambiguous		3G

S2C WT ©

Specimen S2C WT: embryonic day 12.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: red - Ctnnb1; green - Lamc1 (assay MGI:8351675).

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present		S2C WT

S2C Lats1/2/Yap1^PanKOTaz^Het ©

Specimen S2C Lats1/2/Yap1^PanKOTaz^Het: embryonic day 12.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0, Wwtr1^tm1.1Eno/Wwtr1⁺, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: red - Ctnnb1; green - Lamc1 (assay MGI:8351675).

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present		S2C Lats1/2/Yap1^PanKOTaz^Het	Normal expression was detected in Lats1/2/Yap1<PanKO>Taz<Het> mice.

S2C Lats1/2^PanKO ©

Specimen S2C Lats1/2^PanKO: embryonic day 12.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: red - Ctnnb1; green - Lamc1 (assay MGI:8351675).

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present		S2C Lats1/2^PanKO

S3E WT ©

Specimen S3E WT: embryonic day 14.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: magenta - Ctnnb1; green - Nkx6-1 (assay MGI:8351682).

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		S3E WT

S3E Lats1/2^PanKO ©

Specimen S3E Lats1/2^PanKO: embryonic day 14.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: magenta - Ctnnb1; green - Nkx6-1 (assay MGI:8351682).

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		S3E Lats1/2^PanKO

S4C WT row 1 ©

S4C WT row 2 ©

S4C WT row 3 ©

Specimen S4C WT: embryonic day 11.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: magenta - Ctnnb1; cyan - Golga2 (assay MGI:8352276); green - Prkci (assay MGI:8352277).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Present		S4C WT row 2 , S4C WT row 3 , S4C WT row 1

Specimen S4C Lats1/2^PanKO: embryonic day 11.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: magenta - Ctnnb1; cyan - Golga2 (assay MGI:8352276); green - Prkci (assay MGI:8352277).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Present		S4C Lats1/2^PanKO row 2 , S4C Lats1/2^PanKO row 3 , S4C Lats1/2^PanKO row 1

S10B WT ©

Specimen S10B WT: embryonic day 15.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: red - Ctnnb1; green - Hif1a (assay MGI:8352258); magenta - Sox9 (assay MGI:8351676).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		S10B WT

Specimen S10B Lats1/2^PanKO: embryonic day 15.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: red - Ctnnb1; green - Hif1a (assay MGI:8352258); magenta - Sox9 (assay MGI:8351676).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Ambiguous		S10B Lats1/2^PanKO

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