Hif1a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279716 Braitsch CM, et al., LATS1/2 suppress NFkappaB and aberrant EMT initiation to permit pancreatic progenitor differentiation. PLoS Biol. 2019 Jul;17(7):e3000382
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8352258
Gene symbol:	Hif1a
Gene name:	hypoxia inducible factor 1, alpha subunit

Antibody:	anti-Hif1a (H1alpha67) (NB100-105)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

S10B WT ©

Specimen S10B WT: embryonic day 15.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: green - Hif1a; red - Ctnnb1 (assay MGI:8351284); magenta - Sox9 (assay MGI:8351676).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Ambiguous		S10B WT

Specimen S10B Lats1/2^PanKO: embryonic day 15.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: green - Hif1a; red - Ctnnb1 (assay MGI:8351284); magenta - Sox9 (assay MGI:8351676).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		S10B Lats1/2^PanKO	Expression was increased in Lats1/2<PanKO> pancreas.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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