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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8270622
Gene symbol: Ppp1r1b
Gene name: protein phosphatase 1, regulatory inhibitor subunit 1B
Antibody: Anti-DARPP-32 (H-62: sc-11365)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
Results
Specimen 2c: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Ppp1r1b; green - Bcl11b (assay MGI:8270623).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2c , 2c' Expression is detected in striatal projection neurons and is co-labeled with Bcl11b.
Specimen 2d: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Ppp1r1b; green - Bcl11b (assay MGI:8270623).
Structure Level Pattern Image Note
TS26: corpus striatum Present Clusters 2d , 2d' Expression appears in clusters in mutant striatum, which are not labeled by Bcl11b.
Specimen 2e: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Ppp1r1b; green - L1cam (assay MGI:8270624).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2e' , 2e Expression is detected in striatal projection neurons.
Specimen 2f: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Ppp1r1b; green - L1cam (assay MGI:8270624).
Structure Level Pattern Image Note
TS26: corpus striatum Present Clusters 2f , 2f' Expression appears in clusters in Nolz1-/- mutant striatum that are positively labelled by L1cam, indicating these clusters represent abnormally fasciculated axonal tracts.
Specimen 2o: embryonic day 18.5 (more )
Note: Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 2o Expression is detected in striatal projection neurons.
Specimen 2p: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: corpus striatum Weak Clusters 2p Expression was reduced in Nolz1-/- mutant embryos.
Specimen 2q: embryonic day 18.5 (more )
Note: Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: diencephalon Present Regionally restricted 2q Expression was detected in striatal axons projecting towards the midbrain.
TS26: midbrain Present Regionally restricted 2q Expression was detected in striatal axons projecting towards the midbrain.
Specimen 2r: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: diencephalon Absent 2r Striatal axons projecting towards the midbrain were absent in the mutant.
TS26: midbrain Absent 2r Striatal axons projecting towards the midbrain were absent in the mutant.
Specimen 2s: embryonic day 18.5 (more )
Note: Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: diencephalon Present Regionally restricted 2s Expression was detected in striatal axons projecting towards the midbrain.
TS26: midbrain Present Regionally restricted 2s Expression was detected in striatal axons innervating dopaminergic neurons in the midbrain.
Specimen 2t: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: diencephalon Absent 2t Striatal axons projecting towards the midbrain were absent in the mutant.
TS26: midbrain Absent 2t Striatal axons innervating dopaminergic axons in the midbrain were absent in the Nolz1-/- mutant embryos.
Specimen 5j: embryonic day 18.5 (more )
Note: Controls were either: Cre+, Cre+;Nolz1fl/+, or Nolz1fl/fl. Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted 5j Expression is detected in striatal projection neurons.
Specimen 5k: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: red - Ppp1r1b; green - Slc6a3 (assay MGI:8270447).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Weak 5k
Specimen 6g: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hypothalamus Present Regionally restricted 6g Expression was detected in striatonigral projections in the hypothalamus.
Specimen 6h: embryonic day 14.5; Pcdh10tm1Lex/Pcdh10tm1Lex (more )
Structure Level Pattern Image Note
TS22: hypothalamus Ambiguous 6h A lack of striatonigral projections was observed in the Pcdh10-/- hypothalamus.
Specimen S6a: embryonic day 15.5 (more )
Note: Double labeled: red - Ppp1r1b; green - Nefm (assay MGI:8273417).
Structure Cell Type Level Pattern Image Note
TS23: corpus striatum striatum neuron Present Regionally restricted S6a Expression is detected in striatal projection neurons.
Specimen S6b: embryonic day 15.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Ppp1r1b; green - Nefm (assay MGI:8273417).
Structure Level Pattern Image Note
TS23: corpus striatum Ambiguous S6b
Specimen S6c: embryonic day 15.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: red - Ppp1r1b; green - Nefm (assay MGI:8273417).
Structure Level Pattern Image Note
TS23: corpus striatum Ambiguous S6c
Specimen S6d: embryonic day 18.5 (more )
Note: Double labeled: red - Ppp1r1b; green - Nefm (assay MGI:8273417).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Regionally restricted S6d Expression is detected in striatal projection neurons.
Specimen S6e: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Ppp1r1b; green - Nefm (assay MGI:8273417).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Clusters S6e Expression appears in clusters in mutant striatum.
Specimen S6f: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: red - Ppp1r1b; green - Nefm (assay MGI:8273417).
Structure Cell Type Level Pattern Image Note
TS26: corpus striatum striatum neuron Present Clusters S6f
Specimen S7b WT: embryonic day 14.5 (more )
Note: Double labeled: red - Ppp1r1b; green - L1cam (assay MGI:8270624).
Structure Cell Type Level Pattern Image Note
TS22: corpus striatum striatum neuron Present S7b WT
Specimen S7b Pcdh10-/-: embryonic day 14.5; Pcdh10tm1Lex/Pcdh10tm1Lex (more )
Note: Double labeled: red - Ppp1r1b; green - L1cam (assay MGI:8270624).
Structure Cell Type Level Pattern Image Note
TS22: corpus striatum striatum neuron Present S7b Pcdh10-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
MGI 6.29 		The Jackson Laboratory