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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8273417
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament (clone 2H3)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
Results
Specimen S6a: embryonic day 15.5 (more )
Note: Double labeled: green - Nefm; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS23: corpus striatum Present Regionally restricted S6a Axonal tracts were immunolabeled.
Specimen S6b: embryonic day 15.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - Nefm; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS23: corpus striatum Present Regionally restricted S6b Immunolabelled axonal tracts are disorganized and fasciculate abnormally in Nolz1-/- mutant embryos.
Specimen S6c: embryonic day 15.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: green - Nefm; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS23: corpus striatum Present Regionally restricted S6c Immunolabelled axonal tracts are disorganized and fasciculate abnormally in FoxG1-IRESCre;Nolz1<fl/fl> mutant embryos.
Specimen S6d: embryonic day 18.5 (more )
Note: Double labeled: green - Nefm; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6d Axonal tracts were immunolabeled.
Specimen S6e: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - Nefm; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6e Immunolabelled axonal tracts are disorganized and fasciculate abnormally in Nolz1-/- mutant embryos.
Specimen S6f: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: green - Nefm; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6f Immunolabelled axonal tracts are disorganized and fasciculate abnormally in FoxG1-IRESCre;Nolz1<fl/fl> mutant embryos.
Specimen S7c WT: embryonic day 14.5 (more )
Note: Double labeled: green - Nefm; red - Zfp503 (assay MGI:8270488).
Structure Level Pattern Image Note
TS22: corpus striatum Present S7c WT
Specimen S7c Pcdh10-/-: embryonic day 14.5; Pcdh10tm1Lex/Pcdh10tm1Lex (more )
Note: Double labeled: green - Nefm; red - Zfp503 (assay MGI:8270488).
Structure Level Pattern Image Note
TS22: corpus striatum Present S7c Pcdh10-/- Defects in the formation thalamocortical projections as shown by immunolabelling.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
MGI 6.29 		The Jackson Laboratory