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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8270624
Gene symbol: L1cam
Gene name: L1 cell adhesion molecule
Antibody: anti-NCAM L1 (clone 324) (MAB5272)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
Results
Specimen 2e: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: green - L1cam; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS26: corpus striatum Present 2e' , 2e
Specimen 2f: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - L1cam; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS26: corpus striatum Present Clusters 2f' , 2f There was abnormal overlap between L1cam and Ppp1r1b staining in clusters in Nolz1<bgal/bgal> mutant striatum, indicating these clusters represent abnormally fasciculated axonal tracts.
Specimen S6g: embryonic day 18.5 (more )
Note: Double labeled: green - L1cam; red - Th (assay MGI:8270443).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6g Axonal tracts were immunolabeled.
Specimen S6h: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - L1cam; red - Th (assay MGI:8270443).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6h Immunolabelled axonal tracts are disorganized and fasciculate abnormally in Nolz1-/- mutant embryos.
Specimen S6i: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: green - L1cam; red - Th (assay MGI:8270443).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6i Immunolabelled axonal tracts are disorganized and fasciculate abnormally in FoxG1-IRESCre;Nolz1<fl/fl> mutant embryos.
Specimen S6j: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6j Axonal tracts were immunolabeled.
Specimen S6k: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6k Immunolabelled axonal tracts are disorganized and fasciculate abnormally in Nolz1-/- mutant embryos.
Specimen S6l: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6l Immunolabelled axonal tracts are disorganized and fasciculate abnormally in FoxG1-IRESCre;Nolz1<fl/fl> mutant embryos.
Specimen S6m: embryonic day 18.5 (more )
Note: Double labeled: green - L1cam; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: thalamus Present Regionally restricted S6m Expression was detected in thalamocortical axons.
Specimen S6n: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - L1cam; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: thalamus Present Regionally restricted S6n L1cam immunolabelling shows that thalamocortical axons are normally formed in Nolz1-/- mutant embryos but fail to extend into the striatum. There was a strong increase in cleaved Casp3 expression in L1cam-labelled thalamic axons that project towards the striatum in Nolz1-/- mutant embryos.
Specimen S7b WT: embryonic day 14.5 (more )
Note: Double labeled: green - L1cam; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS22: corpus striatum Present S7b WT
Specimen S7b Pcdh10-/-: embryonic day 14.5; Pcdh10tm1Lex/Pcdh10tm1Lex (more )
Note: Double labeled: green - L1cam; red - Ppp1r1b (assay MGI:8270622).
Structure Level Pattern Image Note
TS22: corpus striatum Present S7b Pcdh10-/- Defects in the formation thalamocortical projections as shown by immunolabelling.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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