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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8206372
Gene symbol: Lhx3
Gene name: LIM homeobox protein 3
Antibody: Anti-Lhx3
Results
Specimen 1B brachial (Pbx1): embryonic day 11.5 (more )
Note: Double labeled: green - Lhx3; red - Pbx1 (assay MGI:8206352).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted 1B Pbx1 Lhx3 brachial Expression was detected in medial motor column neurons. Pbx1 colocalized with staining.
Specimen 1B thoracic (Pbx1): embryonic day 11.5 (more )
Note: Double labeled: green - Lhx3; red - Pbx1 (assay MGI:8206352).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted 1B Pbx1 Lhx3 thoracic Expression was detected in medial motor column neurons. Pbx1 colocalized with staining.
Specimen 1C brachial (Pbx3): embryonic day 11.5 (more )
Note: Double labeled: green - Lhx3; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted 1C Pbx3 Lhx3 Expression was detected in medial motor column neurons at brachial levels. Pbx3 was excluded from Lhx3+ medial motor column neurons.
Specimen 4B1: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B1
Specimen 4B2: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B2
Specimen 4B3: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B3
Specimen 4B4: embryonic day 12.5 (more )
Note: Serial thoracic sections. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4B4
Specimen 4D1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D1 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4D2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D2 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4D3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D3 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4D4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Serial thoracic sections. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 4D4 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations. This was observed at all rostrocaudal levels of the spinal cord and was particularly prominent at thoracic levels.
Specimen 4F1: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F1
Specimen 4F2: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F2
Specimen 4F3: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F3
Specimen 4F4: embryonic day 12.5 (more )
Note: Brachial sections are from segments C6-C8. Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4F4
Specimen 4H1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H1 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations.
Specimen 4H2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H2 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations.
Specimen 4H3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H3 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations.
Specimen 4H4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Brachial sections are from segments C6-C8. Double labeled: red - Lhx3; green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 4H4 The distribution of motor neurons in PbxMNdelta mutants revealed a striking disorganization in their settling position. Lhx3+ cells were intermixed with other motor neuron populations.
Specimen 4K Control top: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - horseradish peroxidase retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord medial motor column neuron Present 4K Control HRP Lhx3
Specimen 4K PbxMNdelta top: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Lhx3; green - horseradish peroxidase retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord medial motor column neuron Present 4K PbxMNdelta HRP Lhx3
Specimen 4K Control bottom: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Lhx3; red - rhodamine dextran retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord medial motor column neuron Present 4K Control RhD Lhx3
Specimen 4K PbxMNdelta bottom: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Lhx3; red - rhodamine dextran retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord medial motor column neuron Present 4K PbxMNdelta RhD Lhx3
Specimen 7A brachial (Mecom): embryonic day 12.5 (more )
Note: Double labeled: red - Lhx3; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7A Lhx3 Mecom Expression was detected in medial motor column neurons. A subset of neurons colocalized with Mecom+ neurons.
Specimen 7B thoracic (Mecom): embryonic day 12.5 (more )
Note: Double labeled: red - Lhx3; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present 7B Lhx3 Mecom Expression was detected in medial motor column neurons. A subset of neurons colocalized with Mecom+ neurons.
Specimen 7C Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Lhx3; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7C Control Lhx3 Mecom
Specimen 7C PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Lhx3; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present 7C PbxMNdelta Lhx3 Mecom
Specimen S1B thoracic: embryonic day 11.5 (more )
Note: Double labeled: green - Lhx3; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present S1B Pbx3 Lhx3 thoracic
Specimen S4A1: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4A2: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4A3: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4A4: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4A4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4B1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4B2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4B3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4B4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4B4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4C1: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4C2: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4C3: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4C4: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4C4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4D1: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4D2: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D2 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4D3: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4D4: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4D4 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+); the number of labeled neurons was not significantly altered in the PbxMNdelta mice.
Specimen S4E thoracic Foxp1+/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1+ (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4E1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4E thoracic Foxp1-/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1tm1Eem (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4E2 Isl1/2 Hb9 Lhx3 In the absence of Foxp1, the remaining medial motor column neurons were clustered and well segregated.
Specimen S4E brachial Foxp1+/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1+ (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4E3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4E brachial Foxp1-/-: embryonic day 12.5; Foxp1tm1Eem/Foxp1tm1Eem (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4E4 Isl1/2 Hb9 Lhx3 In the absence of Foxp1, the remaining medial motor column neurons were clustered and well segregated.
Specimen S4F thoracic HoxA/HoxC+/+: embryonic day 12.5 (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4F1 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4F thoracic HoxA/HoxCdelta/delta: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10tm1Mrc, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord medial motor column neuron Present Regionally restricted S4F2 Isl1/2 Hb9 Lhx3 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining medial motor column neurons was unaffected.
Specimen S4F brachial HoxA/HoxC+/+: embryonic day 12.5 (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4F3 Isl1/2 Hb9 Lhx3 Expression was detected in medial motor column neurons (Mnx1+;Lhx3+).
Specimen S4F brachial HoxA/HoxCdelta/delta: embryonic day 12.5; Hoxa10tm1Mrc/Hoxa10tm1Mrc, Hoxc10tm1Mrc/Hoxc10tm1Mrc (more )
Note: Triple labeled: blue - Lhx3; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778); green - Mnx1 (assay MGI:8207842).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord medial motor column neuron Present Regionally restricted S4F4 Isl1/2 Hb9 Lhx3 In combined HoxA/HoxC mutants, the clustering and segregation of the remaining medial motor column neurons was unaffected.
Specimen S4G Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord interneuron Present Regionally restricted S4G Control middle Expression was in ventral interneurons (V2a).
Specimen S4G PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord interneuron Present Regionally restricted S4G PbxMNdelta middle The organization and specification of ventral interneurons was not affected in PbxMNdelta mice.
Specimen S4H Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Lhx3; red - RhD-retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord medial motor column neuron Present S4H Control right
Specimen S4H PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Lhx3; red - RhD-retrograde label.
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord medial motor column neuron Present S4H PbxMNdelta right Retrograde labeling from the ulnar nerve is observed in Lhx3(-) neurons, which are dispersed within the spinal cord of the PbxMNdelta mice.
Specimen S7A e10: embryonic day 10.0 (more )
Structure Cell Type Level Pattern Image Note
TS16: future spinal cord Present Regionally restricted S7A e10 Expression was detected in mature motor neurons, which co-expressed Mecom. Expression was detected in motor neuron precursors, and V2 interneurons, which did not co-express Mecom.
TS16: future spinal cord spinal cord motor neuron Present S7A e10 Expression was detected in mature motor neurons, which co-expressed Mecom.
TS16: future spinal cord spinal cord interneuron Present Regionally restricted S7A e10 Expression was detected in V2 interneurons, which did not co-express Mecom.
Specimen S7A e10.5: embryonic day 10.5 (more )
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted S7A e10.5 Expression was detected in mature motor neurons, which co-expressed Mecom. Expression was detected in motor neuron precursors, and V2 interneurons, which did not co-express Mecom.
TS17: future spinal cord spinal cord motor neuron Present S7A e10.5 Expression was detected in mature motor neurons, which co-expressed Mecom.
TS17: future spinal cord spinal cord interneuron Present Regionally restricted S7A e10.5 Expression was detected in V2 interneurons, which did not co-express Mecom.
Specimen S7A e11.5: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S7A e11.5 Expression was detected in mature motor neurons, which co-expressed Mecom. Expression was detected in motor neuron precursors, and V2 interneurons, which did not co-express Mecom.
TS19: future spinal cord spinal cord motor neuron Present S7A e11.5 Expression was detected in mature motor neurons, which co-expressed Mecom.
TS19: future spinal cord spinal cord interneuron Present Regionally restricted S7A e11.5 Expression was detected in V2 interneurons, which did not co-express Mecom.
Specimen S7B right: embryonic day 12.5 (more )
Note: Rostral cervical level section. Double labeled: red - Lhx3; green - Mecom (assay MGI:8209108).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord cervical region spinal cord medial motor column neuron Present S7B right
TS20: spinal cord cervical region spinal cord motor neuron Present Regionally restricted S7B right Expression was detected in a population of non-medial motor column (non-MMC) neurons. The non-MMC neurons did not co-express Mecom.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory