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Gene Expression Data
Assay Details
Assay
Reference: J:321982 De A, et al., The beta8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development. Development. 2022 Mar 15;149(6):dev200472
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281838
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Antibody: Anti-GFAP
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The antibody used in this assay was not specified; two antibodies were listed: MGI:1314977 and MGI:7281834.
Results
Specimen 7C: postnatal month 7; Tg(Nes-cre)1Kln/0 (more )
Note: Triple labeled: green - Gfap; red - Pecam1 (assay MGI:7281812); blue - Aif1 (assay MGI:7281836).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte of the cerebral cortex Present 7C lower panels
Specimen 7D: postnatal month 7; Tg(Nes-cre)1Kln/0 (more )
Note: Triple labeled: green - Gfap; red - Pecam1 (assay MGI:7281812); blue - Aif1 (assay MGI:7281836).
Structure Cell Type Level Pattern Image Note
TS28: thalamus astrocyte Present 7D lower panels
Specimen 7E: postnatal month 7; Tg(Nes-cre)1Kln/0 (more )
Note: Triple labeled: green - Gfap; red - Pecam1 (assay MGI:7281812); blue - Aif1 (assay MGI:7281836).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum astrocyte of the cerebellum Present 7E lower panels
Specimen 7F: postnatal month 7; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Triple labeled: green - Gfap; red - Pecam1 (assay MGI:7281812); blue - Aif1 (assay MGI:7281836).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte of the cerebral cortex Present 7F lower panels No differences were noted in the mutant.
Specimen 7G: postnatal month 7; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Triple labeled: green - Gfap; red - Pecam1 (assay MGI:7281812); blue - Aif1 (assay MGI:7281836).
Structure Cell Type Level Pattern Image Note
TS28: thalamus astrocyte Present 7G lower panels No differences were noted in the mutant.
Specimen 7H: postnatal month 7; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Triple labeled: green - Gfap; red - Pecam1 (assay MGI:7281812); blue - Aif1 (assay MGI:7281836).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum astrocyte of the cerebellum Present 7H lower panels No differences were noted in the mutant.
Specimen S9G lower panel: postnatal day 16; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Gfap; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte of the cerebral cortex Present S9G lower panel Increased numbers of Gfap+ astrocytes were noted in the mutant.
Specimen S9H lower panel: postnatal day 16; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Gfap; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS28: corpus striatum astrocyte of the forebrain Present S9H lower panel Increased numbers of Gfap+ astrocytes were noted in the mutant.
Specimen S9I lower panel: postnatal day 16; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Gfap; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum astrocyte of the cerebellum Present S9I lower panel Increased numbers of Gfap+ astrocytes were noted in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory