Itgb8^tm2Lfr
Targeted Allele Detail

Summary

• Symbol: Itgb8^tm2Lfr
• Name: integrin beta 8; targeted mutation 2, Louis F Reichardt
• MGI ID: MGI:3608910
• Synonyms: beta8^flox, Itgb8^fl, itgbeta8^flox
• Gene: Itgb8 Location: Chr12:119121757-119202537 bp, - strand Genetic Position: Chr12, 63.48 cM
• Alliance: Itgb8^tm2Lfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102190
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A pgk-neo flanked by FRT sites with a loxP site at the 3' end was inserted into intron 4. A second loxP site was inserted into intron 3. Crossing with mice expressing FLP recombinase removed the pgk-neo, leaving mice with a flanked exon 4. Exon 4 encodes the integrin I-like domain. Cre-mediated deletion of this exon would result in a translational frameshift that generates a premature stop codon. (J:102190)

Expression

In Mice Carrying this Mutation:	43 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itgb8 Mutation: 44 strains or lines available

References

• Original: J:102190 Proctor JM, et al., Vascular development of the brain requires beta8 integrin expression in the neuroepithelium. J Neurosci. 2005 Oct 26;25(43):9940-8
• All: 31 reference(s)