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Gene Expression Data
Assay Details
Assay
Reference: J:321982 De A, et al., The beta8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development. Development. 2022 Mar 15;149(6):dev200472
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281812
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Assay notes: The antibody used in this assay was not specified; two antibodies were listed: MGI:1328239 and MGI:5451027.
Results
Specimen 3D: embryonic day 14.5 (more )
Note: Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 3D Sample was from the ganglionic eminence region.
Specimen 3E: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 3E Sample was from the ganglionic eminence region. Staining indicated abnormal, distended blood vessel morphologies as well as disrupted contacts with perivascular neuroepithelial cells.
Specimen 3F: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 3F Sample was from the ganglionic eminence region. Staining indicated abnormal, distended blood vessel morphologies as well as disrupted contacts with perivascular neuroepithelial cells.
Specimen 3L: embryonic day 14.5 (more )
Note: Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS22: midbrain endothelial cell Present 3L Sample was from the midbrain.
Specimen 3M: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS22: midbrain endothelial cell Present 3M Sample was from the midbrain. The Pecam+ blood vessels have abnormal morphologies and show increased juxtaposition with Aif1+ microglia.
Specimen 3N: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS22: midbrain endothelial cell Present 3N Sample was from the midbrain. The Pecam+ blood vessels have abnormal morphologies and show increased juxtaposition with Aif1+ microglia.
Specimen 4A: embryonic day 14.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4A Sample was from the cerebral cortex.
Specimen 4B: embryonic day 14.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4B Sample was from the midbrain.
Specimen 4C: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4C Sample was from the cerebral cortex. Abnormal Pecam+ blood vessel morphologies were noted.
Specimen 4D: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4D Sample was from the midbrain. Abnormal Pecam+ blood vessel morphologies were noted.
Specimen 4H: embryonic day 14.5 (more )
Note: Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4H Sample was from the cerebral cortex.
Specimen 4I: embryonic day 14.5 (more )
Note: Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4I Sample was from the midbrain.
Specimen 4J: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4J Sample was from the cerebral cortex. Abnormal Pecam+ blood vessel morphologies were noted.
Specimen 4K: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4K Sample was from the midbrain. Abnormal Pecam+ blood vessel morphologies were noted.
Specimen 7C: postnatal month 7; Tg(Nes-cre)1Kln/0 (more )
Note: Triple labeled: red - Pecam1; blue - Aif1 (assay MGI:7281836); green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present 7C lower panels Sample was from cerebral cortex.
Specimen 7D: postnatal month 7; Tg(Nes-cre)1Kln/0 (more )
Note: Triple labeled: red - Pecam1; blue - Aif1 (assay MGI:7281836); green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present 7D lower panels Sample was from thalamus.
Specimen 7E: postnatal month 7; Tg(Nes-cre)1Kln/0 (more )
Note: Triple labeled: red - Pecam1; blue - Aif1 (assay MGI:7281836); green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present 7E lower panels Sample was from cerebellum.
Specimen 7F: postnatal month 7; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Triple labeled: red - Pecam1; blue - Aif1 (assay MGI:7281836); green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present 7F lower panels Sample was from cerebral cortex. No differences were noted in the mutant.
Specimen 7G: postnatal month 7; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Triple labeled: red - Pecam1; blue - Aif1 (assay MGI:7281836); green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present 7G lower panels Sample was from thalamus. No differences were noted in the mutant.
Specimen 7H: postnatal month 7; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Triple labeled: red - Pecam1; blue - Aif1 (assay MGI:7281836); green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present 7H lower panels Sample was from cerebellum. No differences were noted in the mutant.
Specimen S5A cortex: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5A Cortex Sample was from cerebral cortex.
Specimen S5A midbrain: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5A Midbrain Sample was from midbrain.
Specimen S5B cortex: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5B Cortex Sample was from cerebral cortex. E18 mutants continued defects in the juxtaposition between Pecam+ endothelial cells and Nes+ neuroepithelial cells.
Specimen S5B midbrain: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5B Midbrain Sample was from midbrain. E18 mutants continued defects in the juxtaposition between Pecam+ endothelial cells and Nes+ neuroepithelial cells.
Specimen S5C cortex: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex endothelial cell Present S5C Cortex Sample was from cerebral cortex.
Specimen S5C midbrain: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Nes (assay MGI:7281811).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5C Midbrain Sample was from midbrain.
Specimen S5D cortex: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5D Cortex Sample was from cerebral cortex.
Specimen S5D midbrain: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5D Midbrain Sample was from midbrain.
Specimen S5E cortex: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex endothelial cell Present S5E Cortex Sample was from cerebral cortex. Pecam+ cerebral blood vessels were associated with increased numbers of Aif1+ microglia.
Specimen S5E midbrain: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5E Midbrain Sample was from midbrain.
Specimen S5F cortex: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5F Cortex Sample was from cerebral cortex.
Specimen S5F midbrain: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Aif1 (assay MGI:7281813).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5F Midbrain Sample was from midbrain.
Specimen S5G cortex: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Cspg4 (assay MGI:7281871).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5G Cortex Sample was from cerebral cortex.
Specimen S5G midbrain: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Cspg4 (assay MGI:7281871).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5G Midbrain Sample was from midbrain.
Specimen S5H cortex: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cspg4 (assay MGI:7281871).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5H Cortex Sample was from cerebral cortex. Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Specimen S5H midbrain: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cspg4 (assay MGI:7281871).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5H Midbrain Sample was from midbrain. Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Specimen S5I cortex: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Cspg4 (assay MGI:7281871).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5I Cortex Sample was from cerebral cortex. Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Specimen S5I midbrain: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Cspg4 (assay MGI:7281871).
Structure Cell Type Level Pattern Image Note
TS26: blood vessel endothelial cell Present S5I Midbrain Sample was from midbrain. Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Specimen S6A,B: embryonic day 14.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present S6A , S6B Sample was from the telencephalic region.
Specimen S6C,D: embryonic day 14.5; Itgb8tm2Lfr/Itgb8tm2Lfr, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Smad3 (assay MGI:7281830).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present S6C , S6D Sample was from the telencephalic region.
Specimen S9G lower panel: postnatal day 16; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present S9G lower panel Sample was from cerebral cortex.
Specimen S9H lower panel: postnatal day 16; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present S9H lower panel Sample was from corpus striatum.
Specimen S9I lower panel: postnatal day 16; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: red - Pecam1; green - Gfap (assay MGI:7281838).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell Present S9I lower panel Sample was from cerebellum.
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03/25/2025
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