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Gene Expression Data
Assay Details
Assay
Reference: J:321982 De A, et al., The beta8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development. Development. 2022 Mar 15;149(6):dev200472
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281811
Gene symbol: Nes
Gene name: nestin
Antibody: Anti-Nestin (CH23001)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3D: embryonic day 14.5 (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS22: ganglionic eminence Present 3D Expression was detected in neuroepithelial cells. Staining indicated Pecam+ blood vessels closely juxtapose Nes+ neuroepithelium.
Specimen 3E: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS22: ganglionic eminence Present 3E Expression was detected in neuroepithelial cells. Staining indicated abnormal, distended blood vessel morphologies as well as disrupted contacts with perivascular neuroepithelial cells.
Specimen 3F: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS22: ganglionic eminence Present 3F Expression was detected in neuroepithelial cells. Staining indicated abnormal, distended blood vessel morphologies as well as disrupted contacts with perivascular neuroepithelial cells.
Specimen S5A cortex: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S5A Cortex Expression was detected in neuroepithelial cells.
Specimen S5A midbrain: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: midbrain Present S5A Midbrain Expression was detected in neuroepithelial cells.
Specimen S5B cortex: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S5B Cortex Expression was detected in neuroepithelial cells. E18 mutants continued defects in the juxtaposition between Pecam+ endothelial cells and Nes+ neuroepithelial cells.
Specimen S5B midbrain: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: midbrain Present S5B Midbrain Expression was detected in neuroepithelial cells. E18 mutants continued defects in the juxtaposition between Pecam+ endothelial cells and Nes+ neuroepithelial cells.
Specimen S5C cortex: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S5C Cortex Expression was detected in neuroepithelial cells.
Specimen S5C midbrain: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Nes; red - Pecam1 (assay MGI:7281812).
Structure Level Pattern Image Note
TS26: midbrain Present S5C Midbrain Expression was detected in neuroepithelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory