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Gene Expression Data
Assay Details
Assay
Reference: J:266583 Baumgartner M, et al., Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development. 2018 Aug 28;145(17):dev166322
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6400789
Gene symbol: H2ax
Gene name: H2A.X variant histone
Results
Specimen 4D ctrl: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Casp3 (assay MGI:6400589).
Structure Level Pattern Image Note
TS20: neocortex Absent 4D E12 ctrl
Specimen 4D mut: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Casp3 (assay MGI:6400589).
Structure Level Pattern Image Note
TS20: neocortex Present 4D E12 mut The majority of H2ax-immunopositive cells in the mutant pallium were pyknotic, and 93.2% were also cleaved-Casp3-positive.
Specimen 4E E11 control: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Trp53 (assay MGI:6400915).
Structure Level Pattern Image Note
TS20: neocortex Absent 4E E11 ctrl
Specimen 4E E11 mutant: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Trp53 (assay MGI:6400915).
Structure Level Pattern Image Note
TS20: neocortex Ambiguous 4E E11 mut
Specimen 4E E12 control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Trp53 (assay MGI:6400915).
Structure Level Pattern Image Note
TS20: neocortex Absent 4E E12 ctrl
Specimen 4E E12 mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Trp53 (assay MGI:6400915).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4E E12 mut 74.7% of H2ax-immunopositive cells in the mutant pallium were also Trp53-positive.
Specimen 4F left: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Pax6 (assay MGI:6400919).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4F Pax6 46.3% of H2ax-immunopositive cells in the mutant pallium were also Pax6-immunopositive radial glial cells.
Specimen 4F middle: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Eomes (assay MGI:6400773).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4F Tbr2 H2ax-immunopositive cells in the mutant pallium were not Tbr2-immunopositive intermediate progenitor cells.
Specimen 4F right: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - Rbfox3 (assay MGI:6400724).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4F NeuN Virtually no H2ax-immunopositive cells in the mutant pallium were Rbfox3-immunopositive neurons (5.1%).
Specimen 6A: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - H2ax; magenta - EdU.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 6A 38.5% of H2ax-immunopositive cells were also EdU+, similar to the total percentage of EdU+ cells in the mutant pallium.
Specimen S5: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - H2ax; green - phospho-Histone H3.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S5 No H2ax-immunopositive cells were phospho-Histone H3-positive.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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