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Gene Expression Data
Assay Details
Assay
Reference: J:266583 Baumgartner M, et al., Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development. 2018 Aug 28;145(17):dev166322
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6400915
Gene symbol: Trp53
Gene name: transformation related protein 53
Results
Specimen 4E E11 control: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Trp53; magenta - H2ax (assay MGI:6400789).
Structure Level Pattern Image Note
TS20: neocortex Absent 4E E11 ctrl
Specimen 4E E11 mutant: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Trp53; magenta - H2ax (assay MGI:6400789).
Structure Level Pattern Image Note
TS20: neocortex Absent 4E E11 mut
Specimen 4E E12 control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Trp53; magenta - H2ax (assay MGI:6400789).
Structure Level Pattern Image Note
TS20: neocortex Ambiguous 4E E12 ctrl
Specimen 4E E12 mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Trp53; magenta - H2ax (assay MGI:6400789).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4E E12 mut Significantly more cells upregulated Trp53 throughout the E12 Rnu11-null pallium compared with the control. Only 6.7% of Trp53-upregulating cells were also H2ax-immunopositive in the mutant pallium.
Specimen 4G mut: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Trp53; green - Pax6 (assay MGI:6400725).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4G The vast majority of Trp53-upregulating cells in the mutant pallium were Pax6-immunopositive radial glial cells, a significant enrichment compared with the percentage of Pax6-immunopositive radial glial cells in the mutant pallium (93.6%).
Specimen 6B: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Trp53; magenta - EdU.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 6B Approximately one third of Trp53-upregulating cells were also EdU+, a significantly lower percentage than the total percentage of EdU+ cells present in the E12 mutant pallium (43.3%).
Specimen 6C: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Trp53; green - Aurkb (assay MGI:6400916).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 6C Quantification of cells with G2-specific Aurkb staining in the E12 mutant pallium revealed that 14.4% of Trp53-upregulating cells were in G2 phase, a significantly lower percentage than expected.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory