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Gene Expression Data
Assay Details
Assay
Reference: J:266583 Baumgartner M, et al., Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development. 2018 Aug 28;145(17):dev166322
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6400725
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti PAX6 (DSHB)
Assay notes: Two Pax6 antibodies were used; the one used in this assay was not specified. Either MGI:5759939 or MGI:6400552 was used.
Results
Specimen 3E control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3E control Expression was detected in radial glial cells.
Specimen 3E mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3E mutant The number of immunopositive radial glial cells was not changed in the E12 mutant pallium.
Specimen 3F control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted 3F control Expression was detected in radial glial cells.
Specimen 3F mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted 3F mutant At E13 the number of immunopositive radial glial cells was significantly reduced in the mutant pallium. Widespread cytoplasmic expression was observed in the mutant, which was rare in the control sections.
Specimen 3G control: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: neocortex Present Regionally restricted 3G control Expression was detected in radial glial cells.
Specimen 3G mutant: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: neocortex Present Single cells 3G mutant At E14 there were few immunopositive radial glial cells in the mutant pallium.
Specimen 5G control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Pax6; magenta - EdU.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 5G E12 ctrl Expression was detected in radial glial cells.
Specimen 5G mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Pax6; magenta - EdU.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 5G E12 mut There was a significantly lower percentage of EdU+/Pax6+ radial glial cells in the mutant compared with control.
Specimen S4E control: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Pax6; magenta - EdU.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S4E ctrl Expression was detected in radial glial cells.
Specimen S4E mutant: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Pax6; magenta - EdU.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S4E mut There was no change in the percentage of EdU+/Pax6+ radial glial cells in the E11 mutant pallium compared with the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory