About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:266583 Baumgartner M, et al., Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development. 2018 Aug 28;145(17):dev166322
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6400724
Gene symbol: Rbfox3
Gene name: RNA binding protein, fox-1 homolog (C. elegans) 3
Results
Specimen 3A control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3A control Expression was detected in neurons.

Specimen 3A mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3A mutant The number of immunopositive neurons was not changed in the E12 mutant pallium.

Specimen 3B control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted 3B control Expression was detected in neurons.

Specimen 3B mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted 3B mutant The number of immunopositive neurons was not changed in the E13 mutant pallium.

Specimen 3C control: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS22: neocortex Present Regionally restricted 3C control Expression was detected in neurons.

Specimen 3C mutant: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS22: neocortex Present Regionally restricted 3C mutant At E14 the number of immunopositive neurons was significantly reduced in the mutant pallium.

Specimen 4F right: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Rbfox3; magenta - H2ax (assay MGI:6400789).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4F NeuN Expression was detected in neurons in the mutant pallium. There was virtually no co-expression with H2ax (5.1%).

Specimen S2A E12 control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S2A E12 ctrl top , S2A E12 ctrl bottom Expression was detected in neurons.

Specimen S2A E12 mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S2A E12 mut bottom , S2A E12 mut top Rnu11 FISH in conjunction with immunolabeling showed the presence of Rnu11-null neural progenitor cells (Mki67+) and Rnu11-null neurons (Rbfox3+) in the mutant pallium.

Specimen S2A E13 control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2A E13 ctrl bottom , S2A E13 ctrl top Expression was detected in neurons.

Specimen S2A E13 mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2A E13 mut bottom , S2A E13 mut top Rnu11 FISH in conjunction with immunolabeling showed the presence of Rnu11-null neural progenitor cells (Mki67+) and Rnu11-null neurons (Rbfox3+) in the mutant pallium.

Specimen S2F control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Rbfox3; magenta - BrdU.
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2F ctrl Expression was detected in neurons.

Specimen S2F mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Rbfox3; magenta - BrdU.
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2F mut There was a signficant decrease in Rbfox3+/BrdU+ cells as a percentage of all Rbfox3-positive cells in the E13 mutant pallium compared with the control.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/22/2024
MGI 6.24
The Jackson Laboratory