Analysis Tools
hematopoietic system
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• the proportion of single HSCs that give rise to clones of more than 50 cells after 7 days in culture is higher and single HSCs show earlier cell division and form larger clones, however, the percentages of LSK, lineage-positive, Mac1+, CD41+, and CD71+ cells are unaltered
• while HSCs exhibit increased proliferation in vitro, the long-term in vivo repopulating activity of bone marrow cells in competitive bone marrow transplantation is normal
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• mice treated with poly I:C exhibit compromised erythropoiesis in the bone marrow
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• a degree of extramedullary hematopoiesis is seen in some cases of poly I:C injected mice
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• mice treated with poly I:C exhibit an increase in megakaryopoiesis
• however, numbers of myeloerythroid progenitor populations and more mature myeloid cells (Mac1+ Ly6g+) in the bone marrow are not altered
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• bone marrow of poly I:C treated mice shows megakaryocytic hyperplasia, with megakaryocytes displaying large and hyperlobated nuceli and increased clustering
• no significant alternation in the proportion of megakaryocytes or myeloid cells is seen in the spleen
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• bone marrow of poly I:C contains increased numbers of colony-forming unit megakaryocytes
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• bone marrow of poly I:C injected mice contains reduced numbers of CD71+Ter119+ erythroblasts
• however, no significant alternation in the proportion of erythroblasts is seen in the spleen
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• mice induced with polyinosinic:polycytidylic acid (poly I:C) injection 6 to 8 weeks after birth develop thrombocytosis
• thrombocytosis is transplantable: wild-type recipient mice transplanted with bone marrow cells from heterozygous mice 2 months after poly I:C develop thrombocytosis and recipients also develop thrombocytosis after secondary transplantations
• platelets show normal response to several agonists including collagen-related peptide, thrombin, and adenosine 5-diphosphate
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• white blood cell counts are mildly elevated in some, but not all, cohorts of poly I:C treated mice
• however, red blood cell parameters are not affected
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• bone marrow of poly I:C injected mice contains increased numbers of hematopoietic stem cells (HSCs), both Lin-Sca1+cKit+CD150+CD48- HSCs and E-SLAM HSCs (CD45+EPCR+CD150+CD48-)
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immune system
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• white blood cell counts are mildly elevated in some, but not all, cohorts of poly I:C treated mice
• however, red blood cell parameters are not affected
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cellular
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• the proportion of single HSCs that give rise to clones of more than 50 cells after 7 days in culture is higher and single HSCs show earlier cell division and form larger clones, however, the percentages of LSK, lineage-positive, Mac1+, CD41+, and CD71+ cells are unaltered
• while HSCs exhibit increased proliferation in vitro, the long-term in vivo repopulating activity of bone marrow cells in competitive bone marrow transplantation is normal
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| essential thrombocythemia | DOID:2224 |
OMIM:187950 OMIM:601977 OMIM:614521 |
J:262170 | |
