Tg(VAV1-cre)1Graf
Transgene Detail

Summary

• Symbol: Tg(VAV1-cre)1Graf
• Name: transgene insertion 1, Thomas Graf
• MGI ID: MGI:3765313
• Synonyms: vav-Cre
• Transgene: Tg(VAV1-cre)1Graf Location: unknown
• Alliance: Tg(VAV1-cre)1Graf page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(VAV1-cre)1Graf expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Vav1

• Mutation details: A bicistronic sequence coding for Cre recombinase and YFP linked by an IRES domain was placed downstream of human VAV1 promoter sequence and upstream of VAV1 enhancer sequence. The entire construct was flanked by two 250 bp core elements from chicken beta globulin insulator. Transgenic mice failed to express YFP but crossing with a Gt(ROSA)26Sor^tm1(EYFP)Cos reporter strained revealed that 70% of mice had cre expression restricted to hematopoietic cells, including hematopoietic stem cells. The remaining 30% had cre expression in a wider range of cells, possibly due to transient expression of the transgene in early blastomeres. (J:127936)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Vav1 (species not specified) Summary of all recombinase alleles driven by Vav1. Comparative matrix view of recombinase activities

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:127936 Stadtfeld M, et al., Assessing the role of hematopoietic plasticity for endothelial and hepatocyte development by non-invasive lineage tracing. Development. 2005 Jan;132(1):203-13
• All: 107 reference(s)