Analysis Tools|
Allele Symbol Allele Name Allele ID |
Dppa3tm1(cre)Peli targeted mutation 1, Pentao Liu MGI:5004882 |
||||||||||||||||||||||||
| Summary |
5 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• untreated mice show significantly increased mean spleen weight
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 for 2 months restores spleen weight to wild-type levels
|
|
• untreated mice show significantly increased mean spleen weight
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 for 2 months restores spleen weight to wild-type levels
|
|
• untreated mice show increased platelet number in peripheral blood
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 for 1 or 2 months leads to a significant reduction in peripheral platelet number
|
|
• untreated mice show increased leukocyte number in peripheral blood
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 for 1 or 2 months leads to a significant reduction in peripheral leukocyte number
|
|
• untreated mice show significantly increased % and absolute number of LSK+ cells in the bone marrow
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 for 2 months restores % and absolute number of LSK+ cells in the bone marrow to wild-type levels
|
|
• mice develop myeloproliferative neoplasm (MPN)-associated hematologic phenotypes
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 ameliorates MPN-related phenotypes
|
|
• mice exhibit a 50% reduction in ZFAND2B protein levels and a 2.2-fold increase in IGF1R (insulin-like growth factor I receptor) protein levels relative to wild-type controls
• Zfand2b mRNA levels are reduced in the bone marrow
|
|
• untreated mice show significantly increased mean spleen weight
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 for 2 months restores spleen weight to wild-type levels
|
|
• untreated mice show increased leukocyte number in peripheral blood
• daily treatment with IGF1R kinase inhibitor NVP-AEW541 for 1 or 2 months leads to a significant reduction in peripheral leukocyte number
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• at E12.5, male mice show no differences in primordial germ cell (PGC) numbers relative to wild-type males, suggesting that the observed age-dependent loss of germ cells may not have an embryonic origin
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
N |
• male mice are born in expected Mendelian ratios and exhibit normal survival up to 12 months of age
|
|
|
• males show an age-dependent loss of germ cells, starting at 3 months of age
• however, the frequency of cleaved-caspase 3-positive apoptotic germ cells is not significantly altered at 6 months of age, suggesting that meiosis can proceed normally and that spermatocytes are not cleared by apoptosis
|
|
|
• starting at 3 months of age, males show a time-dependent decrease in the number of PLZF+ undifferentiated spermatogonia (USGs) per seminiferous tubule, along with an age-dependent increase in the proportion of tubules lacking any PLZF+ USGs
• however, age-dependent loss of USGs is not driven by increased apoptosis or premature differentiation of USGs
|
|
|
• at 10 weeks of age, testes show reduced protein expression of ZBTB16/PLZF [a marker of undifferentiated spermatogonia (USGs)], suggesting that maintenance of the USG pool, which comprises spermatogonial stem cells (SSCs) and progenitor germ cells, is not preserved
• in contrast, expression of WT1 (a Sertoli cell marker) is relatively normal, suggesting that that the Sertoli cell population is unaffected
|
|
|
• males show a time-dependent increase in seminiferous tubules lacking germ cells (% of Sertoli cell-only tubules)
• starting at 3 months of age, the number of PLZF+ cells per tubule is significantly lower than in wild-type controls, whereas the % of tubules lacking any PLZF+ undifferentiated spermatogonia (USGs) is significantly increased
|
|
|
• testis weight is normal at 6 weeks of age but decreases over time, starting at 3 months of age; by 12 months of age, testis weight is reduced by ~40%
|
|
|
• testes are grossly smaller by 12 months of age
|
|
|
• although initially intact, spermatogenesis becomes compromised upon aging
|
|
|
• after 3 months of age, males show a time-dependent decrease in epididymal sperm concentration, indicating reduced sperm production upon aging
• however, seminal vesicle weight and serum testosterone levels remain unaffected up to 12 months of age, and remaining sperm are functional with no significant impact on male fertility
|
|
|
• aged undifferentiated spermatogonia (USGs) replicate less due to impaired S-phase progression
|
|
N |
• mouse embryonic stem cells (mESCs) derived from male embryos show normal sensitivity to DNA-protein crosslink-inducing agents, as indicated by normal cell survival after exposure to formaldehyde (which crosslinks a broad spectrum of proteins to DNA) or etoposide (which induces DNA-Topoisomerase 2 crosslinks)
• 2D cell cycle analysis indicates normal distribution of mESCs at each stage of the cell cycle (G1, S, and G2)
|
|
|
• males show an age-dependent loss of germ cells, starting at 3 months of age
• however, the frequency of cleaved-caspase 3-positive apoptotic germ cells is not significantly altered at 6 months of age, suggesting that meiosis can proceed normally and that spermatocytes are not cleared by apoptosis
|
|
|
• after 3 months of age, males show a time-dependent decrease in epididymal sperm concentration, indicating reduced sperm production upon aging
• however, seminal vesicle weight and serum testosterone levels remain unaffected up to 12 months of age, and remaining sperm are functional with no significant impact on male fertility
|
|
|
• starting at 3 months of age, males show a time-dependent decrease in the number of PLZF+ undifferentiated spermatogonia (USGs) per seminiferous tubule, along with an age-dependent increase in the proportion of tubules lacking any PLZF+ USGs
• however, age-dependent loss of USGs is not driven by increased apoptosis or premature differentiation of USGs
|
|
|
• males fail to show an age-dependent increase in the frequency (%) of quiescent undifferentiated spermatogonia (Ki67-negative PLZF+ USGs), unlike wild-type males
• surprisingly, >6-month-old males do not exhibit an increased % of pH3-positive PLZF+ USGs, indicating that more USGs are engaged in the cell cycle but are not dividing more frequently
• at >6 months of age, the % of CCNA2-positive PLZF+ USGs is significantly higher than in wild-type males, with no change detected at 1.5 months, suggesting an accumulation in S/G2
• moreover, the % of EdU-positive PLZF+ USGs is significantly higher at 6 months of age, suggesting that USGs specifically accumulate in the S-phase
• however, the % of gamma-H2AX-positive PLZF+ USGs and the gamma-H2AX signal intensity per USG are not changed at 1.5 or 6 months of age, suggesting that USGs are not accumulating in S-phase due to DNA breaks
|
|
|
• aged undifferentiated spermatogonia (USGs) replicate less due to impaired S-phase progression
|
|
|
• at 10 weeks of age, testes show reduced protein expression of ZBTB16/PLZF [a marker of undifferentiated spermatogonia (USGs)], suggesting that maintenance of the USG pool, which comprises spermatogonial stem cells (SSCs) and progenitor germ cells, is not preserved
• in contrast, expression of WT1 (a Sertoli cell marker) is relatively normal, suggesting that that the Sertoli cell population is unaffected
|
|
|
• males show a time-dependent increase in seminiferous tubules lacking germ cells (% of Sertoli cell-only tubules)
• starting at 3 months of age, the number of PLZF+ cells per tubule is significantly lower than in wild-type controls, whereas the % of tubules lacking any PLZF+ undifferentiated spermatogonia (USGs) is significantly increased
|
|
|
• testis weight is normal at 6 weeks of age but decreases over time, starting at 3 months of age; by 12 months of age, testis weight is reduced by ~40%
|
|
|
• testes are grossly smaller by 12 months of age
|
|
N |
• male mice exhibit normal body weight up to 12 months of age
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• female mice are born in Mendelian ratios and exhibit normal fertility with no significant reduction in the number of follicles per ovary section at 2 and 6 months of age
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 09/30/2025 MGI 6.24 |
|
|
|
||
