Phenotypes associated with this allele

• Allele Symbol: Tg(APOA1)1Rub
• Allele Name: transgene insertion 1, Edward M Rubin
• Allele ID: MGI:3052540
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Apoa1^tm1Unc/Apoa1^tm1Unc Tg(APOA1)1Rub/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3783803
cx2	Abca1^tm1Jdm/Abca1^tm1Jdm Abcg1^tm1Dgen/Abcg1^tm1Dgen Tg(APOA1)1Rub/0	involves: 129P2/OlaHsd * C57BL/6 * DBA * DBA/1LacJ	MGI:5451014
cx3	Tg(APOA1)1Rub/0 Tg(APOA2)3Rub/0	involves: C57BL/6	MGI:3783805
cx4	Tg(APOA1)1Rub/? Tg(APOA2)3Rub/?	involves: C57BL/6	MGI:3800811
cx5	Naxe^tm1.1Lfan/Naxe^tm1.1Lfan Tg(APOA1)1Rub/0	involves: C57BL/6 * C57BL/6J	MGI:6278513
cx6	Tg(AlbLCAT)1Jdm/0 Tg(APOA1)1Rub/0	involves: C57BL/6 * FVB/N	MGI:3835376
cx7	Tg(AlbLCAT)1Jdm/0 Tg(APOA1)1Rub/0 Tg(APOA2)3Rub/0	involves: C57BL/6 * FVB/N	MGI:3835379
tg8	Tg(APOA1)1Rub/Tg(APOA1)1Rub	C57BL/6-Tg(APOA1)1Rub/J	MGI:3581585
tg9	Tg(APOA1)1Rub/0	involves: C57BL/6	MGI:3835375

Genotype
MGI:3783803

cx1

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc; Tg(APOA1)1Rub/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased circulating cholesterol level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased circulating HDL cholesterol level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased circulating triglyceride level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased cholesterol efflux ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

Genotype
MGI:5451014

cx2

• Allelic Composition: Abca1^tm1Jdm/Abca1^tm1Jdm; Abcg1^tm1Dgen/Abcg1^tm1Dgen; Tg(APOA1)1Rub/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA * DBA/1LacJ

hematopoietic system

abnormal hematopoietic system physiology ( J:190966 )

• in bone marrow transplants experiments, hematopoietic stem and multipotential progenitor cell (HSPC) exhibit reduced mobilization compared with Abca1^tm1Jdm Abcg1^tm1Dgen double homozygotes

Genotype
MGI:3783805

cx3

• Allelic Composition: Tg(APOA1)1Rub/0; Tg(APOA2)3Rub/0
• Genetic Background: involves: C57BL/6

homeostasis/metabolism

decreased circulating cholesterol level ( J:121992 )

decreased circulating HDL cholesterol level ( J:121992 )

increased circulating cholesterol level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased circulating HDL cholesterol level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased circulating triglyceride level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased cholesterol efflux ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

Genotype
MGI:3800811

cx4

• Allelic Composition: Tg(APOA1)1Rub/?; Tg(APOA2)3Rub/?
• Genetic Background: involves: C57BL/6

homeostasis/metabolism

abnormal lipid homeostasis ( J:92450 , J:129704 )

• HDL particle size has a broad size distribution between 8 and 9.5 nm but is missing the 10.5 nm particles observed in mice carrying just the Tg(APOA1)1Rub transgene (J:92450)

• HDL particle size has a broad size distribution similar to human HDL particles that contain apoA-I and apoA-II (J:129704)

cardiovascular system

decreased susceptibility to atherosclerosis ( J:129704 )

• there is a 2.7-fold reduction in the mean lesion area found in the aorta of mice fed an atherogenic diet for 36 weeks compared to wild-type mice

Genotype
MGI:6278513

cx5

• Allelic Composition: Naxe^tm1.1Lfan/Naxe^tm1.1Lfan; Tg(APOA1)1Rub/0
• Genetic Background: involves: C57BL/6 * C57BL/6J

cardiovascular system

cardiovascular system phenotype ( J:261549 )

N • as anticipated, increased HDL levels reverse the retinal proangiogenic phenotype, as determined by the degree of radial extension of the vascular plexus, vascular branch points, number of endothelial tip cells, and filopodia in P5 retinas; no differences in vascular remodeling are observed relative to Naxe^tm1.1Lfan homozygotes

N • consistent with this rescue, P5 retinas show reduced VEGFR2 activation, elevated NICD protein levels, and significantly increased Hey1, Hey2, and Hes1 mRNA levels relative to Naxe^tm1.1Lfan homozygotes

homeostasis/metabolism

increased circulating HDL cholesterol level ( J:261549 )

Genotype
MGI:3835376

cx6

• Allelic Composition: Tg(AlbLCAT)1Jdm/0; Tg(APOA1)1Rub/0
• Genetic Background: involves: C57BL/6 * FVB/N

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:128254 )

N • despite being fed an atherogenic diet, mice exhibit no change in total cholesterol, triglyceride and HDL-cholesterol levels compared to similarly treated wild-type mice

increased circulating cholesterol level ( J:121992 )

• up to 4.2-fold compared to in Tg(APOA1)1Rub mice

increased circulating HDL cholesterol level ( J:121992 )

• 2-fold compared to in Tg(AlbLCAT)1Jdm or Tg(APOA1)1Rub mice

increased circulating LDL cholesterol level ( J:121992 )

increased circulating VLDL cholesterol level ( J:121992 )

cardiovascular system

increased susceptibility to atherosclerosis ( J:128254 )

• atherosclerotic lesions are 3-fold larger than in Tg(APOA1)1Rub mice

Genotype
MGI:3835379

cx7

• Allelic Composition: Tg(AlbLCAT)1Jdm/0; Tg(APOA1)1Rub/0; Tg(APOA2)3Rub/0
• Genetic Background: involves: C57BL/6 * FVB/N

homeostasis/metabolism

increased circulating cholesterol level ( J:121992 )

• 2-fold compared to in Tg(APOA1)1Rub Tg(APOA2)3Rub mice

increased circulating HDL cholesterol level ( J:121992 )

• 2-fold compared to in Tg(AlbLCAT)1Jdm or Tg(APOA1)1Rub Tg(APOA2)3Rub mice

Genotype
MGI:3581585

tg8

• Allelic Composition: Tg(APOA1)1Rub/Tg(APOA1)1Rub
• Genetic Background: C57BL/6-Tg(APOA1)1Rub/J

homeostasis/metabolism

abnormal circulating interleukin-17 level ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit less of an increase in plasma IL17 compared with control mice

abnormal lipid homeostasis ( J:92450 , J:129704 )

• HDL particle size has a bimodal distribution with two peaks at 8.5 and 10.5 nm compared to the single peak at 9.9 nm for non-transgenic controls (J:92450)

• 90% of HDL particles contain human apoA-I instead of endogenous murine apo-AI (J:129704)

• HDL particle size has a bimodal distribution with two peaks similar to human HDL particles that contain apoA-I (J:129704)

abnormal lipid level ( J:42425 )

• total APOA1 (mouse and human ) is increased two fold

• mouse APOA1 is reduced 95%

• mouse APOA2 is reduced 45%

• HDL particles include two subsets of larger (10.2-10.8nm) and smaller particles (8.4-8.6nm) as compared to control; these sizes fall with the range for human HDL-2b and HDL-3a, respectively

abnormal circulating HDL cholesterol level ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit less of a decrease in HDL cholesterol compared with control mice

increased circulating cholesterol level ( J:42425 )

• HDL cholesterol is increased two fold

increased circulating HDL cholesterol level ( J:42425 )

increased cholesterol efflux ( J:107390 )

• in macrophages in vitro

cardiovascular system

decreased susceptibility to atherosclerosis ( J:99552 , J:107390 , J:129704 )

• reduced susceptibility to diet-induced fatty streak lesions (J:99552)

• macrophages transplanted into Apoa1^tm1Unc homozygotes or bone marrow transplanted into Apoa1^tm1Unc homozygotes in conjunction with Apoe^tm1Unc bone marrow confers resistance to diet-induced atherosclerosis (J:107390)

• there is a greater than 40-fold reduction in the mean lesion area found in the aorta of mice fed an atherogenic diet for 36 weeks compared to wild-type mice (J:129704)

hematopoietic system

decreased hematopoietic stem cell number ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit less of an increase in LSK cells in the spleen compared with control mice

increased hematopoietic stem cell number ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit an increase in LSK cells in the bone marrow compared with control mice

abnormal spleen weight ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit less of an increase in spleen weight compared with control mice

abnormal hematopoietic system physiology ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit less of an increase in hematopoietic stem and multipotential progenitor cell (HSPC) mobilization in compared with control mice

immune system

abnormal spleen weight ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit less of an increase in spleen weight compared with control mice

abnormal circulating interleukin-17 level ( J:190966 )

• in bone marrow transplants experiments, mice receiving bone marrow transduced with an Flt3-ITD-expressing retrovirus exhibit less of an increase in plasma IL17 compared with control mice

Genotype
MGI:3835375

tg9

• Allelic Composition: Tg(APOA1)1Rub/0
• Genetic Background: involves: C57BL/6

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:128254 )

N • despite being fed an atherogenic diet, mice exhibit no change in total cholesterol, triglyceride and HDL-cholesterol levels compared to similarly treated wild-type mice

decreased circulating triglyceride level ( J:121992 )

cardiovascular system

decreased susceptibility to atherosclerosis ( J:128254 )

• atherosclerotic lesions are 3-fold smaller than in Tg(APOA1)1Rub Tg(AlbLCAT)1Jdm or Tg(APOA1)1Rub Tg(CETP)5203Tall mice and 4-fold smaller than in Tg(AlbLCAT)1Jdm mice

• the risk of developing atherosclerotic lesions greater than 50th percentile is 4.3-fold lower than in wild-type mice